Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,801 G→C 5.3% A337A (GCG→GCC cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,8010GC 5.3% 343.2 / 5.0 110A337A (GCG→GCCcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (54/50);  new base C (4/2);  total (58/52)
Fisher's exact test for biased strand distribution p-value = 6.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

GATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGG  >  Exported/385733‑385864
                                                                    |                                                               
gATTGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgt                                                               <  1:5600569/71‑1 (MQ=255)
gATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgt                                                               <  1:2073417/71‑1 (MQ=255)
  tGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgtgt                                                             <  1:381716/71‑1 (MQ=255)
  tGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgtg                                                              >  1:757379/1‑70 (MQ=255)
   ggCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTc                                                            >  1:3297794/1‑71 (MQ=255)
    gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTc                                                            <  1:3043332/70‑1 (MQ=255)
    gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCt                                                           <  1:208726/71‑1 (MQ=255)
    gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCt                                                           <  1:2961331/71‑1 (MQ=255)
    gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCt                                                           >  1:1195516/1‑71 (MQ=255)
    gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCt                                                           <  1:3771173/71‑1 (MQ=255)
     cACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTg                                                          >  1:1514140/1‑71 (MQ=255)
      aCTGGCCCGGTGTGGTCGGGCAATGTGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCgtc                                                              <  1:3076186/66‑2 (MQ=255)
      aCTGGCCCGGTGTGGTCGGGCAATGTGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCgtc                                                              <  1:5583916/66‑2 (MQ=255)
       cTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGc                                                        >  1:1656502/1‑71 (MQ=255)
       cTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGc                                                        >  1:3721522/1‑71 (MQ=255)
                tATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTc                                                     <  1:4039023/65‑1 (MQ=255)
                  tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgt                                                               <  1:5547667/53‑1 (MQ=255)
                  tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGc                                                        <  1:5280871/60‑1 (MQ=255)
                    gACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTg                                            <  1:1045122/70‑1 (MQ=255)
                    gACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTg                                            <  1:4515735/70‑1 (MQ=255)
                     aCCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCAGTGGCGCGGTGTCTgg                                                         <  1:3608653/56‑1 (MQ=255)
                       cGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTc                                                            <  1:4457964/51‑1 (MQ=255)
                          tAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtctt                                     <  1:3370116/71‑1 (MQ=255)
                          tAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtctt                                     <  1:4003777/71‑1 (MQ=255)
                           aaCCTGTTCGCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttc                                    >  1:3309006/1‑71 (MQ=255)
                           aaCCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTg                                            >  1:1059454/1‑63 (MQ=255)
                           aaCCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttc                                    >  1:5359783/1‑71 (MQ=255)
                             tcTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGgt                                                               <  1:652291/41‑1 (MQ=38)
                              cTGTTCCCGTTCCTGTTTTTCACCATCGCCTGTGGCGCGGTGTCTGGCTTc                                                     <  1:754599/51‑1 (MQ=255)
                              cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTc                                                     <  1:705027/51‑1 (MQ=255)
                              cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTc                                                     <  1:423090/51‑1 (MQ=255)
                              cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTc                                                     <  1:1546709/51‑1 (MQ=255)
                              cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg                                 <  1:4010256/71‑1 (MQ=255)
                              cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg                                 <  1:3770457/71‑1 (MQ=255)
                              cTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGgt                                                               <  1:1652347/41‑1 (MQ=38)
                              cTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGAt                                          >  1:3604457/1‑62 (MQ=255)
                              cTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCgg                                 >  1:3630361/1‑71 (MQ=255)
                              cTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCgg                                 >  1:344535/1‑71 (MQ=255)
                               tGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg                                 >  1:316871/1‑70 (MQ=255)
                               tGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCtcttccg                                  <  1:379824/69‑3 (MQ=255)
                               tGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTcc                                                    >  1:3721054/1‑51 (MQ=19)
                               tGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCa                                                   >  1:179118/1‑52 (MQ=25)
                               tGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCa                                                   >  1:204728/1‑52 (MQ=25)
                                gTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCa                                                   >  1:3396405/1‑51 (MQ=255)
                                 ttCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAc                              >  1:2880985/1‑71 (MQ=255)
                                  tcgcGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGAc                              >  1:2441857/4‑70 (MQ=255)
                                  tCCCGTTCCTGTTCATCACCATCGCCTGTGGtgcg                                                                 >  1:1075373/1‑35 (MQ=37)
                                   cccGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTc                                                     >  1:1554944/1‑46 (MQ=255)
                                   cccGTTCATGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTc                                                     >  1:1115606/1‑46 (MQ=255)
                                    ccGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCAc                           <  1:5355415/71‑1 (MQ=255)
                                      gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGc                                                        >  1:2734362/1‑40 (MQ=37)
                                      gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc                         >  1:4703036/1‑71 (MQ=255)
                                      gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc                         >  1:5588902/1‑71 (MQ=255)
                                      gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc                         >  1:201029/1‑71 (MQ=255)
                                       ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGcc                        <  1:168692/71‑1 (MQ=255)
                                       ttCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGcc                        <  1:3677951/71‑1 (MQ=255)
                                         ccTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGa                                           >  1:3575214/1‑50 (MQ=255)
                                          cTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt                                          >  1:4105061/1‑50 (MQ=255)
                                          cTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt                                          >  1:1213713/1‑50 (MQ=255)
                                          cTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt                                          >  1:577523/1‑50 (MQ=255)
                                          cTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGccaa                      <  1:2874781/70‑3 (MQ=255)
                                          cTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGccaa                      <  1:345194/70‑3 (MQ=255)
                                           tGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttct                                   <  1:2502230/56‑1 (MQ=255)
                                             tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATctct                                      <  1:1289056/51‑1 (MQ=255)
                                             tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                   <  1:5116961/70‑1 (MQ=255)
                                             tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                   <  1:1576820/70‑1 (MQ=255)
                                             tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                   <  1:1661703/70‑1 (MQ=255)
                                                 tCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAcga                            <  1:2420229/57‑3 (MQ=255)
                                                   aCCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTgg             >  1:3700686/1‑70 (MQ=255)
                                                   aCCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGc            >  1:4549970/1‑71 (MQ=255)
                                                   aCCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGc            >  1:5469831/1‑71 (MQ=255)
                                                   aCCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGc            >  1:2658140/1‑71 (MQ=255)
                                                    ccATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAcga                            <  1:1188041/54‑3 (MQ=255)
                                                    ccATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGct           <  1:3375521/71‑2 (MQ=255)
                                                       tCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTgg             <  1:4625930/66‑1 (MQ=255)
                                                       tCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaac        >  1:1952477/1‑71 (MQ=255)
                                                       tCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaac        >  1:5309445/1‑71 (MQ=255)
                                                        cGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaat        >  1:1707696/1‑69 (MQ=255)
                                                         gCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatg       >  1:5027682/1‑68 (MQ=255)
                                                         gCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatg       <  1:5170441/70‑3 (MQ=255)
                                                         gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg       <  1:4227084/70‑1 (MQ=255)
                                                         gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg       <  1:3113432/70‑1 (MQ=255)
                                                         gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg       <  1:133727/70‑1 (MQ=255)
                                                         gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg       <  1:2474919/70‑1 (MQ=255)
                                                         gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg       <  1:2238044/70‑1 (MQ=255)
                                                         gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcg           >  1:3418768/1‑66 (MQ=255)
                                                          ccTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatga      >  1:4632422/1‑70 (MQ=255)
                                                          ccTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatga      >  1:1528704/1‑70 (MQ=255)
                                                           cTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAg    >  1:2125439/1‑71 (MQ=255)
                                                              tgGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatgaaacc  <  1:89464/70‑4 (MQ=255)
                                                              tgGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGct           <  1:1943278/61‑2 (MQ=255)
                                                              tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                   >  1:4199975/1‑53 (MQ=255)
                                                              tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                   >  1:2217885/1‑53 (MQ=255)
                                                              tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                   >  1:2359027/1‑53 (MQ=255)
                                                               ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATg                 >  1:5336243/1‑54 (MQ=255)
                                                               ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATg                 >  1:2834309/1‑54 (MQ=255)
                                                               ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTAGGGACCACGCCGAAGATg                 >  1:2771463/1‑53 (MQ=255)
                                                                gtgcgGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaa         >  1:5412112/3‑61 (MQ=255)
                                                                gtgcgGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaa         >  1:36651/3‑61 (MQ=255)
                                                                gtgcgGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGc            >  1:3204708/3‑58 (MQ=255)
                                                                    |                                                               
GATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGG  >  Exported/385733‑385864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: