Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,834 G→T 32.9% G348G (GGG→GGT cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,8340GT32.9% 158.1 / 71.7 100G348G (GGG→GGTcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (35/32);  new base T (18/15);  total (53/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 105 total aligned reads displayed.

TTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAAT  >  Exported/385766‑385904
                                                                    |                                                                      
ttCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAc                                                                      >  1:2880985/1‑71 (MQ=255)
 tcgcGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGAc                                                                      >  1:2441857/4‑70 (MQ=255)
   ccGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCAc                                                                   <  1:5355415/71‑1 (MQ=255)
     gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc                                                                 >  1:5588902/1‑71 (MQ=255)
     gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc                                                                 >  1:4703036/1‑71 (MQ=255)
     gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc                                                                 >  1:201029/1‑71 (MQ=255)
      ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGcc                                                                <  1:168692/71‑1 (MQ=255)
      ttCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGcc                                                                <  1:3677951/71‑1 (MQ=255)
         cTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGccaa                                                              <  1:345194/70‑3 (MQ=255)
         cTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGccaa                                                              <  1:2874781/70‑3 (MQ=255)
            tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                           <  1:1661703/70‑1 (MQ=255)
            tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                           <  1:1576820/70‑1 (MQ=255)
            tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                           <  1:5116961/70‑1 (MQ=255)
                tCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAcga                                                                    <  1:2420229/57‑3 (MQ=255)
                  aCCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTgg                                                     >  1:3700686/1‑70 (MQ=255)
                  aCCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGc                                                    >  1:4549970/1‑71 (MQ=255)
                  aCCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGc                                                    >  1:2658140/1‑71 (MQ=255)
                  aCCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGc                                                    >  1:5469831/1‑71 (MQ=255)
                   ccATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAcga                                                                    <  1:1188041/54‑3 (MQ=255)
                   ccATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGct                                                   <  1:3375521/71‑2 (MQ=255)
                      tCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTgg                                                     <  1:4625930/66‑1 (MQ=255)
                      tCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaac                                                >  1:1952477/1‑71 (MQ=255)
                      tCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaac                                                >  1:5309445/1‑71 (MQ=255)
                       cGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaat                                                >  1:1707696/1‑69 (MQ=255)
                        gCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatg                                               >  1:5027682/1‑68 (MQ=255)
                        gCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatg                                               <  1:5170441/70‑3 (MQ=255)
                        gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                               <  1:3113432/70‑1 (MQ=255)
                        gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                               <  1:2474919/70‑1 (MQ=255)
                        gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                               <  1:2238044/70‑1 (MQ=255)
                        gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                               <  1:133727/70‑1 (MQ=255)
                        gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                               <  1:4227084/70‑1 (MQ=255)
                        gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcg                                                   >  1:3418768/1‑66 (MQ=255)
                         ccTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatga                                              >  1:4632422/1‑70 (MQ=255)
                         ccTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatga                                              >  1:1528704/1‑70 (MQ=255)
                             tgGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatgaaacc                                          <  1:89464/70‑4 (MQ=255)
                             tgGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGct                                                   <  1:1943278/61‑2 (MQ=255)
                             tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                           >  1:5612503/1‑53 (MQ=255)
                             tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                           >  1:4199975/1‑53 (MQ=255)
                             tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                           >  1:3948685/1‑53 (MQ=255)
                             tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                           >  1:2217885/1‑53 (MQ=255)
                             tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                           >  1:2359027/1‑53 (MQ=255)
                              ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATg                                                         >  1:4360710/1‑54 (MQ=255)
                              ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATg                                                         >  1:2834309/1‑54 (MQ=255)
                              ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATg                                                         >  1:5336243/1‑54 (MQ=255)
                              ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTAGGGACCACGCCGAAGATg                                                         >  1:2771463/1‑53 (MQ=255)
                               gtgcgGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaa                                                 >  1:36651/3‑61 (MQ=255)
                               gtgcgGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaa                                                 >  1:5412112/3‑61 (MQ=255)
                               gtgcgGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaa                                                 >  1:3497235/3‑61 (MQ=255)
                               gtgcgGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGc                                                    >  1:3204708/3‑58 (MQ=255)
                                     tgtCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                               <  1:4439594/57‑1 (MQ=255)
                                     tgtCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                               <  1:2946652/57‑1 (MQ=255)
                                     tgtCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGc                                 <  1:510735/71‑1 (MQ=255)
                                       tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                               <  1:1964989/55‑1 (MQ=255)
                                       tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt                               <  1:3530569/71‑1 (MQ=255)
                                       tCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaat                                                <  1:256970/54‑2 (MQ=255)
                                         tGGCTTCCCTGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTa                             >  1:4829790/1‑71 (MQ=255)
                                         tGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTa                             >  1:383174/1‑71 (MQ=255)
                                         tGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatgaaac                                           >  1:3451681/1‑55 (MQ=255)
                                           tcTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGAGAACGAAGGGCAGGCGTGATTTATc                           >  1:1509315/2‑71 (MQ=255)
                                           gCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCttt                              <  1:2768365/68‑1 (MQ=255)
                                           gCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCttt                              <  1:4386772/68‑1 (MQ=255)
                                           gCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATc                           >  1:889385/1‑71 (MQ=255)
                                           gCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaa                                                 >  1:3400887/1‑49 (MQ=255)
                                                cATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTa                      <  1:385124/71‑1 (MQ=255)
                                                cATGCGCTGATCTCTTCTGGGACCACGCAGAAGATGCTGGCGAACGAAGGGCAGGc                                     >  1:4279267/1‑56 (MQ=255)
                                                 acgcgcTGATCTCTTCTGGTACGACGCCAAAACTGCTGGCTAATGAAACCGACGCGCGTTTCATCGGCTAc                     <  1:5073468/69‑1 (MQ=255)
                                                 acgcgcTGATCTCTTCCGGTACGACGCCAAAACTGCTGGCTAATGAAACCGACGCGCGTTTCATCGGCTAc                     <  1:3511640/69‑1 (MQ=255)
                                                 acgcgcTGATCTCTTCCGGTACGACGCCAAAACTGCTGGCTAATGAAACCGACGCGCGTTTCATCGGCTAc                     <  1:3148854/69‑1 (MQ=255)
                                                 aTGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTAc                     <  1:5662606/71‑1 (MQ=255)
                                                   gcgcTGATCTCTTCCGGTACGACGCCAAAACTGCTGGCTAATGAAACCGACGCGCGTTTCATCGGCTACgg                   >  1:1947638/1‑71 (MQ=255)
                                                    cgcTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTAt                            >  1:1328776/1‑61 (MQ=255)
                                                    cgcTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACgg                   >  1:516288/1‑70 (MQ=255)
                                                    cgcTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGt                  >  1:1336817/1‑71 (MQ=255)
                                                     gcTGATCTCTTCTGGGACCACGCCGAAGATGCAGGcgaacga                                              <  1:4413063/42‑1 (MQ=255)
                                                      cTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg                <  1:1495428/71‑1 (MQ=255)
                                                      cTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg                <  1:4844150/71‑1 (MQ=255)
                                                      cTGATCTCTTCCGGTACGACGCCAAAACTGCTGGCTAATGAAACCGACGCGCGTTTCATCGGCTACggcgc                <  1:4059700/71‑4 (MQ=255)
                                                       tGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg                <  1:3426164/70‑1 (MQ=255)
                                                        gATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacgaa                                             >  1:50421/1‑40 (MQ=255)
                                                        gATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacgaa                                             >  1:4415187/1‑40 (MQ=255)
                                                        gATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacgaa                                             >  1:4492211/1‑40 (MQ=255)
                                                         aTCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGAt             >  1:3010674/1‑71 (MQ=255)
                                                           ctctTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACgg                   >  1:3112958/1‑63 (MQ=255)
                                                           ctctTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGt           >  1:1357813/1‑71 (MQ=255)
                                                            tcttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt                               <  1:4180707/50‑1 (MQ=255)
                                                            tcttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt                               <  1:1965076/50‑1 (MQ=255)
                                                             cttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGt           >  1:4686747/1‑69 (MQ=255)
                                                             cttctGCGACCACGCCGAAGATGCTGGAGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGt           >  1:2410232/1‑69 (MQ=255)
                                                              ttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTaa        >  1:25075/1‑71 (MQ=255)
                                                               tctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGtt          <  1:2364841/68‑1 (MQ=255)
                                                                ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa         <  1:2013463/68‑1 (MQ=255)
                                                                ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa         <  1:2760664/68‑1 (MQ=255)
                                                                ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa         <  1:4011975/68‑1 (MQ=255)
                                                                ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa         <  1:4899299/68‑1 (MQ=255)
                                                                ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAAt       >  1:1746415/1‑70 (MQ=255)
                                                                   ggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg                <  1:761870/58‑1 (MQ=255)
                                                                   ggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg     <  1:2247715/69‑1 (MQ=255)
                                                                   ggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg     <  1:5649198/69‑1 (MQ=255)
                                                                    gACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAAt  <  1:440833/71‑1 (MQ=255)
                                                                    gACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAAt  <  1:433986/71‑1 (MQ=255)
                                                                    |                                                                      
TTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAAT  >  Exported/385766‑385904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: