BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	132,247	A→G	24.4%	K180K (AAA→AAG)	yadD →	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	132,247	0	A	G	24.4%	151.0 / 49.0	90	K180K (AAA→AAG)	yadD	predicted transposase
Reads supporting (aligned to +/- strand): ref base A (39/29); new base G (14/8); total (53/37)
Fisher's exact test for biased strand distribution p-value = 6.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

TCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTA  >  Exported/132180‑132317

tCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                                      >  1:4331714/1‑70 (MQ=255)

 cACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCata                                                                    >  1:4456591/1‑71 (MQ=255)

 cACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                                                     >  1:3151006/1‑70 (MQ=255)

 cACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                                                    >  1:137935/1‑71 (MQ=255)

  aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCata                                                                    <  1:1682564/70‑1 (MQ=255)

  aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                                                   <  1:5411175/71‑1 (MQ=255)

     aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCg                                                                <  1:4109234/71‑1 (MQ=255)

      tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                               <  1:2811198/71‑1 (MQ=255)

      tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                               <  1:1990572/71‑1 (MQ=255)

      tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                               >  1:1724271/1‑71 (MQ=255)

      tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                               <  1:3733406/71‑1 (MQ=255)

        acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc                                                              <  1:5225852/70‑1 (MQ=255)

        acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc                                                              <  1:1456262/70‑1 (MQ=255)

            cGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcgc                                                         >  1:5603944/1‑71 (MQ=255)

            cGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcgc                                                         <  1:1982600/71‑1 (MQ=255)

            cGGATGACGAAATCATGCAACATAGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcg                                                          >  1:4314735/1‑70 (MQ=255)

             ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                                                      <  1:4411448/57‑1 (MQ=255)

             ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                                                      <  1:5499548/57‑1 (MQ=255)

                tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                               <  1:5480886/61‑1 (MQ=255)

                tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACt                                                     <  1:5105414/71‑1 (MQ=255)

                  aCGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACtt                                                    >  1:4311932/1‑70 (MQ=255)

                     agaTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTAAtc                                                >  1:5264174/3‑70 (MQ=255)

                      aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                                               <  1:4346016/71‑1 (MQ=255)

                        tCATGCAACATCGGCGTATAGCTCTGCTTGAACTGATTCAAAAGCATATTCGCGACCGCGat                                                      <  1:437409/62‑2 (MQ=255)

                        tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                                                      <  1:3389478/62‑2 (MQ=255)

                        tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                                                      <  1:5381492/62‑2 (MQ=255)

                        tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGAttt                                                    <  1:4614380/64‑1 (MQ=255)

                        tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTAAtcgg                                              <  1:4086207/70‑4 (MQ=255)

                        tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                                                        <  1:3964826/44‑1 (MQ=255)

                        tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                                                        <  1:5304759/44‑1 (MQ=255)

                        tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                                                        <  1:2386059/44‑1 (MQ=255)

                         cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCata                                                                    >  1:1096838/1‑47 (MQ=255)

                         cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                                                     >  1:3007904/1‑46 (MQ=255)

                         cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                                                     >  1:3643736/1‑46 (MQ=255)

                         cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                                                     >  1:3949494/1‑46 (MQ=255)

                         cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                                                      >  1:2371657/1‑60 (MQ=255)

                         cATGCAACATAGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                                                      >  1:3248330/1‑60 (MQ=255)

                          aTGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                                                      >  1:176267/1‑44 (MQ=255)

                            gCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGcga                                                             >  1:2947347/1‑49 (MQ=255)

                             cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcg                                                          >  1:1678405/1‑53 (MQ=255)

                             cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt                                        <  1:4014833/71‑1 (MQ=255)

                             cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt                                        >  1:121453/1‑71 (MQ=255)

                             cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt                                        >  1:1755295/1‑71 (MQ=255)

                              aaCATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                                                   >  1:2550432/1‑59 (MQ=255)

                              aaCATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                                                   >  1:2398642/1‑59 (MQ=255)

                                 aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                                                  >  1:4756399/1‑57 (MQ=255)

                                 aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                                                  >  1:5389881/1‑57 (MQ=255)

                                 aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg                                    >  1:3152257/1‑71 (MQ=255)

                                   cggcggATTGCGATTCTCGAACTACTGCAAAAACatat                                                                   >  1:2487730/1‑38 (MQ=255)

                                   cggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAAt                                                 >  1:4727016/1‑56 (MQ=255)

                                   cggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAAt                                                 >  1:4733468/1‑56 (MQ=255)

                                   cggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAAt                                                 >  1:5388259/1‑56 (MQ=255)

                                    ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                                 <  1:1726524/71‑1 (MQ=255)

                                    ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                                 <  1:1932043/71‑1 (MQ=255)

                                    ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                                 >  1:5025523/1‑71 (MQ=255)

                                    ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                                 >  1:549698/1‑71 (MQ=255)

                                       ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACt                               <  1:1854585/70‑1 (MQ=255)

                                              gATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt                                       <  1:1875035/55‑1 (MQ=255)

                                              gATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt                                       <  1:1957148/55‑1 (MQ=255)

                                                ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCt                      >  1:5450095/1‑70 (MQ=255)

                                                ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTg                     <  1:2192961/71‑1 (MQ=255)

                                                 tctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGa                    >  1:1205416/1‑71 (MQ=255)

                                                 tctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGa                    >  1:3072992/1‑71 (MQ=255)

                                                  ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                                                  >  1:1582883/1‑40 (MQ=255)

                                                  ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                                 >  1:4439828/1‑57 (MQ=255)

                                                  ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                                 >  1:3476834/1‑57 (MQ=255)

                                                     gAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTAt                                            >  1:1217928/1‑43 (MQ=255)

                                                     gAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAt                   <  1:498423/68‑1 (MQ=255)

                                                     gAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATc                  <  1:2636217/69‑1 (MQ=255)

                                                       actactGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgcc               >  1:4945734/1‑68 (MQ=255)

                                                       actactGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgac               <  1:27477/70‑1 (MQ=255)

                                                          actGCAAAAACATATTCGCCAGCGCGACTTTATGt                                               <  1:2813211/35‑1 (MQ=255)

                                                          actGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAg           >  1:4110214/1‑71 (MQ=255)

                                                           ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAAc                                >  1:820289/1‑49 (MQ=255)

                                                           ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAAc                                >  1:2780334/1‑49 (MQ=255)

                                                           ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATc                  <  1:1448313/63‑1 (MQ=255)

                                                           ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGaa            >  1:723550/1‑69 (MQ=255)

                                                           ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAgg          >  1:3767974/1‑71 (MQ=255)

                                                            tGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGc                                   <  1:5630840/45‑1 (MQ=255)

                                                            tGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGt                            <  1:2358735/52‑1 (MQ=255)

                                                             gCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgacga             >  1:5393177/1‑66 (MQ=255)

                                                             gCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgacga             >  1:4396399/1‑66 (MQ=255)

                                                              cAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAt                   >  1:748620/1‑59 (MQ=255)

                                                              cAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAt                   >  1:3749686/1‑59 (MQ=255)

                                                              cAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAt                   >  1:3560665/1‑59 (MQ=255)

                                                              cAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAt                   >  1:671609/1‑59 (MQ=255)

                                                              cAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcga                >  1:692509/1‑62 (MQ=255)

                                                              cAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAggg         <  1:4352719/69‑1 (MQ=255)

                                                               aaaaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAg           >  1:4778198/1‑66 (MQ=255)

                                                               aaaaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTac      <  1:3883655/71‑1 (MQ=255)

                                                               aaaaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTa       <  1:3334884/70‑1 (MQ=255)

                                                               aaaaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTa       <  1:3088165/70‑1 (MQ=255)

                                                               aaaaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCAACGAAg           >  1:3339152/1‑66 (MQ=255)

                                                                 aaaCATATTCGCCAGCGCGACTTAATGTTATTGCtt                                       <  1:1658124/36‑1 (MQ=255)

                                                                 aaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcg                 <  1:279077/58‑1 (MQ=255)

                                                                 aaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTacac    >  1:4318894/1‑71 (MQ=255)

                                                                   aCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTacac    >  1:1876175/1‑69 (MQ=255)

                                                                   aCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTacac    >  1:542714/1‑69 (MQ=255)

                                                                   aCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTacac    >  1:2959748/1‑69 (MQ=255)

                                                                   aCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTa  <  1:3289267/71‑1 (MQ=255)

TCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTA  >  Exported/132180‑132317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`