Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,888 T→C 11.1% G366G (GGT→GGC cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,8880TC11.1% 310.3 / 22.8 121G366G (GGT→GGCcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base T (43/63);  new base C (8/5);  total (52/69)
Fisher's exact test for biased strand distribution p-value = 2.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

Only 100 of 125 total aligned reads displayed.

CGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGG  >  Exported/385818‑385953
                                                                      |                                                                 
  cTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg                                                                 <  1:4844150/71‑1 (MQ=255)
  cTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg                                                                 <  1:1495428/71‑1 (MQ=255)
   tGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg                                                                 <  1:3426164/70‑1 (MQ=255)
     aTCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGAt                                                              >  1:3010674/1‑71 (MQ=255)
       ctctTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGt                                                            >  1:1357813/1‑71 (MQ=255)
         cttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGt                                                            >  1:4686747/1‑69 (MQ=255)
         cttctGCGACCACGCCGAAGATGCTGGAGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGt                                                            >  1:2410232/1‑69 (MQ=255)
            ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa                                                          <  1:3034470/68‑1 (MQ=255)
            ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa                                                          <  1:4899299/68‑1 (MQ=255)
            ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa                                                          <  1:4011975/68‑1 (MQ=255)
            ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa                                                          <  1:2013463/68‑1 (MQ=255)
            ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAAt                                                        >  1:1746415/1‑70 (MQ=255)
               ggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg                                                                 <  1:761870/58‑1 (MQ=255)
               ggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg                                                      <  1:5649198/69‑1 (MQ=255)
               ggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg                                                      <  1:2247715/69‑1 (MQ=255)
                gACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAAt                                                   <  1:433986/71‑1 (MQ=255)
                gACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAAt                                                   <  1:440833/71‑1 (MQ=255)
                  ccACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATcc                                                 <  1:2874289/71‑1 (MQ=255)
                  ccACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATcc                                                 <  1:4484983/71‑1 (MQ=255)
                    aCGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCtt                                               <  1:375380/71‑1 (MQ=255)
                     caccGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTGATGGAATCCtt                                               <  1:1252535/68‑1 (MQ=255)
                     caccGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTGATGGAATCCtt                                               <  1:409881/68‑1 (MQ=255)
                     caccGAAGATGCTGGCGAACGAAGGGCAGACGTGCTTTATCGGCTACGGTGGGATGTTGATGGAATCCtt                                               <  1:5587177/68‑1 (MQ=255)
                     cGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATg                                                             <  1:2363600/56‑1 (MQ=255)
                     cGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTc                                              >  1:3637276/1‑71 (MQ=255)
                      gCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCg                                             <  1:898131/71‑1 (MQ=255)
                       ccGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGt                                            <  1:4641613/71‑1 (MQ=255)
                        cGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg                                                                 <  1:906365/49‑1 (MQ=255)
                              tGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGt                                                                   >  1:1689869/1‑41 (MQ=255)
                              tGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTg                                                                  >  1:3152363/1‑42 (MQ=255)
                              tGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTaa                                                         >  1:3732506/1‑51 (MQ=255)
                              tGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTaa                                                         >  1:5374554/1‑51 (MQ=255)
                              tGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTaa                                                         >  1:3160814/1‑51 (MQ=255)
                              tGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGATACGGTg                                                                  >  1:1601697/1‑42 (MQ=255)
                               gCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATcc                                                 >  1:3680521/1‑58 (MQ=255)
                               gCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                    >  1:1189088/1‑71 (MQ=255)
                               gCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                    >  1:2766293/1‑71 (MQ=255)
                               gCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                    >  1:2620599/1‑71 (MQ=255)
                               gCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                    >  1:2123505/1‑71 (MQ=255)
                                  ggCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTaa                                                         <  1:3026065/47‑1 (MQ=255)
                                   gCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTGATGGAATCCtt                                               <  1:935580/56‑1 (MQ=255)
                                   gCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGgc                                >  1:789094/1‑71 (MQ=255)
                                   gCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGgc                                <  1:746920/71‑1 (MQ=255)
                                     gaacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTGATGGAATCCtt                                               <  1:2906134/54‑1 (MQ=255)
                                      aacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGtt                                                           <  1:1279920/41‑1 (MQ=255)
                                      aacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGtt                                                           <  1:2094552/41‑1 (MQ=255)
                                      aacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGtt                                                           <  1:1339751/41‑1 (MQ=255)
                                      aacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGtt                                                           <  1:2114374/41‑1 (MQ=255)
                                      aacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGtt                                                           <  1:1505732/41‑1 (MQ=255)
                                      aacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCt                             <  1:2447684/71‑1 (MQ=255)
                                        cgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAAt                                                   >  1:4405294/1‑47 (MQ=255)
                                        cgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAAt                                                   >  1:4450174/1‑47 (MQ=255)
                                         gaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGa                                       >  1:5062498/1‑58 (MQ=255)
                                         gaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGa                                       >  1:620664/1‑58 (MQ=255)
                                         gaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGt                          <  1:1472331/71‑1 (MQ=255)
                                         gaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGt                          <  1:4094316/71‑1 (MQ=255)
                                          aaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATg                                                             <  1:2573297/35‑1 (MQ=255)
                                          aaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGtt                         >  1:4509184/1‑71 (MQ=255)
                                             ggCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTcc                      <  1:1768261/71‑1 (MQ=255)
                                             ggCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTcc                      <  1:5588546/71‑1 (MQ=255)
                                               cAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGc                    >  1:4717433/1‑71 (MQ=255)
                                                   agTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGt                <  1:171864/70‑1 (MQ=255)
                                                     tGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCtt                                               <  1:4061539/38‑1 (MQ=255)
                                                     tGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCtt                                               <  1:3634666/38‑1 (MQ=255)
                                                     tGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCtt                                               <  1:5316669/38‑1 (MQ=255)
                                                     tGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTg                                           <  1:63155/42‑1 (MQ=255)
                                                       cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGa                                       >  1:1709021/1‑44 (MQ=255)
                                                       cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatca            <  1:2988911/71‑1 (MQ=255)
                                                       cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatc             <  1:2660878/70‑1 (MQ=255)
                                                       cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatc             <  1:382975/70‑1 (MQ=255)
                                                        tttATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTAt                                   <  1:1986052/47‑1 (MQ=255)
                                                         tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTa                                    >  1:4385773/3‑45 (MQ=255)
                                                         tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc          >  1:5459176/3‑71 (MQ=255)
                                                         tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc          >  1:1024988/3‑71 (MQ=255)
                                                         tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc          >  1:2478888/3‑71 (MQ=255)
                                                         tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc          >  1:4048213/3‑71 (MQ=255)
                                                          tATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGAt                                      <  1:714986/42‑1 (MQ=255)
                                                          tATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTcc                      >  1:5249769/1‑58 (MQ=255)
                                                          caTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc          >  1:2165164/2‑70 (MQ=255)
                                                           aTCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                    >  1:400964/1‑43 (MQ=255)
                                                           aTCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                    >  1:2587767/1‑43 (MQ=255)
                                                           aTCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCg         <  1:2290757/70‑1 (MQ=255)
                                                            tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTc                       >  1:2316601/1‑55 (MQ=255)
                                                            tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGt                >  1:3036305/1‑62 (MQ=255)
                                                            tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGt                >  1:3774717/1‑62 (MQ=255)
                                                            tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatca            <  1:43401/66‑1 (MQ=255)
                                                            tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa        <  1:5618758/70‑1 (MQ=255)
                                                            tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa        <  1:342877/70‑1 (MQ=255)
                                                            tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa        <  1:564175/70‑1 (MQ=255)
                                                            tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa        <  1:3811882/70‑1 (MQ=255)
                                                            tCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGaa       >  1:4965950/1‑70 (MQ=255)
                                                             cGGCTACGGGGGGATGTTAATGGAATCCTTCGTGGCGAt                                      <  1:2418344/39‑1 (MQ=255)
                                                             cGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGAt                                      <  1:5372381/39‑1 (MQ=12)
                                                              ggCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatcatc          >  1:1404056/1‑66 (MQ=255)
                                                              ggCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatcatc          >  1:4375191/1‑66 (MQ=255)
                                                              ggCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGaac      <  1:401342/70‑3 (MQ=255)
                                                              ggCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAAcc     <  1:1386348/71‑1 (MQ=255)
                                                               gCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCg    <  1:396407/71‑1 (MQ=255)
                                                                   cGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCggg  <  1:1775997/69‑1 (MQ=255)
                                                                      tGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTg                 <  1:987350/51‑1 (MQ=255)
                                                                      |                                                                 
CGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGG  >  Exported/385818‑385953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: