Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,890 G→C 12.2% G367A (GGG→GCG) ‡ cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,8900GC12.2% 308.2 / 27.8 115G367A (GGG→GCG) ‡cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (41/60);  new base C (9/5);  total (50/65)
Fisher's exact test for biased strand distribution p-value = 1.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

Only 100 of 121 total aligned reads displayed.

CTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTA  >  Exported/385820‑385959
                                                                      |                                                                     
cTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg                                                                       <  1:4844150/71‑1 (MQ=255)
cTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg                                                                       <  1:1495428/71‑1 (MQ=255)
 tGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg                                                                       <  1:3426164/70‑1 (MQ=255)
   aTCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGAt                                                                    >  1:3010674/1‑71 (MQ=255)
       cttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGt                                                                  >  1:4686747/1‑69 (MQ=255)
       cttctGCGACCACGCCGAAGATGCTGGAGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGt                                                                  >  1:2410232/1‑69 (MQ=255)
        ttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTaa                                                               >  1:25075/1‑71 (MQ=255)
         tctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGtt                                                                 <  1:2364841/68‑1 (MQ=255)
          ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa                                                                <  1:2013463/68‑1 (MQ=255)
          ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa                                                                <  1:4011975/68‑1 (MQ=255)
          ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa                                                                <  1:3034470/68‑1 (MQ=255)
          ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa                                                                <  1:2760664/68‑1 (MQ=255)
          ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAAt                                                              >  1:1746415/1‑70 (MQ=255)
             ggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg                                                                       <  1:761870/58‑1 (MQ=255)
             ggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg                                                            <  1:5649198/69‑1 (MQ=255)
             ggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg                                                            <  1:2247715/69‑1 (MQ=255)
              gACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAAt                                                         <  1:433986/71‑1 (MQ=255)
              gACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAAt                                                         <  1:440833/71‑1 (MQ=255)
                ccACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATcc                                                       <  1:2874289/71‑1 (MQ=255)
                ccACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATcc                                                       <  1:4484983/71‑1 (MQ=255)
                  aCGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCtt                                                     <  1:375380/71‑1 (MQ=255)
                   caccGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTGATGGAATCCtt                                                     <  1:1252535/68‑1 (MQ=255)
                   caccGAAGATGCTGGCGAACGAAGGGCAGACGTGCTTTATCGGCTACGGTGGGATGTTGATGGAATCCtt                                                     <  1:5587177/68‑1 (MQ=255)
                   cGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATg                                                                   <  1:2363600/56‑1 (MQ=255)
                   cGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTc                                                    <  1:1682076/71‑1 (MQ=255)
                   cGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTc                                                    >  1:3637276/1‑71 (MQ=255)
                    gCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCg                                                   <  1:898131/71‑1 (MQ=255)
                    gCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCg                                                   >  1:5626112/1‑71 (MQ=255)
                     ccGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATGCTTCGt                                                  <  1:6528/71‑1 (MQ=255)
                     ccGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGt                                                  <  1:4641613/71‑1 (MQ=255)
                      cGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg                                                                       <  1:906365/49‑1 (MQ=255)
                       gAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATcc                                                       >  1:4508440/1‑64 (MQ=255)
                            tGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTaa                                                               >  1:3732506/1‑51 (MQ=255)
                            tGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTaa                                                               >  1:3160814/1‑51 (MQ=255)
                            tGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTaa                                                               >  1:5374554/1‑51 (MQ=255)
                            tGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTaa                                                               >  1:4227598/1‑51 (MQ=255)
                             gCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                          >  1:1189088/1‑71 (MQ=255)
                             gCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                          >  1:2766293/1‑71 (MQ=255)
                             gCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                          >  1:2620599/1‑71 (MQ=255)
                             gCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                          >  1:2123505/1‑71 (MQ=255)
                                ggCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTaa                                                               <  1:3026065/47‑1 (MQ=255)
                                 gCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTGATGGAATCCtt                                                     <  1:935580/56‑1 (MQ=255)
                                 gCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGgc                                      >  1:789094/1‑71 (MQ=255)
                                 gCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGgc                                      <  1:746920/71‑1 (MQ=255)
                                   gaacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTGATGGAATCCtt                                                     <  1:2906134/54‑1 (MQ=255)
                                    aacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGtt                                                                 <  1:1279920/41‑1 (MQ=255)
                                    aacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGtt                                                                 <  1:2094552/41‑1 (MQ=255)
                                    aacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGtt                                                                 <  1:1339751/41‑1 (MQ=255)
                                     acgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATTGAATCCTTCGTGGCGATTATGGCGCTg                                  >  1:2246043/1‑71 (MQ=255)
                                      cgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAAt                                                         >  1:4405294/1‑47 (MQ=255)
                                      cgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAAt                                                         >  1:4450174/1‑47 (MQ=255)
                                      cgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATc                                                        >  1:3750771/1‑48 (MQ=255)
                                       gaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGa                                             >  1:5062498/1‑58 (MQ=255)
                                       gaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGa                                             >  1:620664/1‑58 (MQ=255)
                                       gaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGt                                <  1:1472331/71‑1 (MQ=255)
                                       gaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGt                                <  1:4094316/71‑1 (MQ=255)
                                        aaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATg                                                                   <  1:2573297/35‑1 (MQ=255)
                                             cAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGc                          >  1:4717433/1‑71 (MQ=255)
                                                gCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTg                       <  1:5210306/71‑1 (MQ=255)
                                                 agTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGt                      <  1:171864/70‑1 (MQ=255)
                                                   tGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCtt                                                     <  1:4061539/38‑1 (MQ=255)
                                                   tGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCtt                                                     <  1:3634666/38‑1 (MQ=255)
                                                   tGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTat                    >  1:5383460/1‑71 (MQ=255)
                                                     cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGa                                             >  1:1709021/1‑44 (MQ=255)
                                                     cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatca                  <  1:2988911/71‑1 (MQ=255)
                                                      tttATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTAt                                         <  1:1986052/47‑1 (MQ=255)
                                                       tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTa                                          >  1:4385773/3‑45 (MQ=255)
                                                       tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                >  1:5459176/3‑71 (MQ=255)
                                                       tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                >  1:1024988/3‑71 (MQ=255)
                                                       tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                >  1:4048213/3‑71 (MQ=255)
                                                       tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                >  1:2478888/3‑71 (MQ=255)
                                                       tcaTCGGCTACGGAGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                >  1:1412700/3‑71 (MQ=255)
                                                        tATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGAt                                            <  1:714986/42‑1 (MQ=255)
                                                        tATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGAt                                            <  1:4782108/42‑1 (MQ=255)
                                                        tATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTcc                            >  1:5249769/1‑58 (MQ=255)
                                                        caTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                >  1:2165164/2‑70 (MQ=255)
                                                         aTCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                          >  1:400964/1‑43 (MQ=255)
                                                         aTCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                          >  1:2587767/1‑43 (MQ=255)
                                                         aTCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCg               <  1:2067750/70‑1 (MQ=255)
                                                          tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTc                             >  1:2316601/1‑55 (MQ=255)
                                                          tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGt                      >  1:3774717/1‑62 (MQ=255)
                                                          tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGt                      >  1:3036305/1‑62 (MQ=255)
                                                          tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatca                  <  1:43401/66‑1 (MQ=255)
                                                          tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa              <  1:342877/70‑1 (MQ=255)
                                                          tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa              <  1:3811882/70‑1 (MQ=255)
                                                          tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa              <  1:5618758/70‑1 (MQ=255)
                                                          tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa              <  1:564175/70‑1 (MQ=255)
                                                          tCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGaa             >  1:4965950/1‑70 (MQ=255)
                                                           cGGCTACGGGGGGATGTTAATGGAATCCTTCGTGGCGAt                                            <  1:2418344/39‑1 (MQ=255)
                                                           cGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGAt                                            <  1:5372381/39‑1 (MQ=12)
                                                            ggCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatcatc                >  1:3924332/1‑66 (MQ=255)
                                                            ggCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatcatc                >  1:1404056/1‑66 (MQ=255)
                                                            ggCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatcatc                >  1:4375191/1‑66 (MQ=255)
                                                            ggCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGaac            <  1:401342/70‑3 (MQ=255)
                                                            ggCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAAcc           <  1:1386348/71‑1 (MQ=255)
                                                             gCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCg          <  1:396407/71‑1 (MQ=255)
                                                             gCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCg          <  1:1752776/71‑1 (MQ=255)
                                                                 cGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCggg        <  1:1775997/69‑1 (MQ=255)
                                                                    tGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTg                       <  1:987350/51‑1 (MQ=255)
                                                                     gggATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTa  >  1:4061144/1‑71 (MQ=255)
                                                                      |                                                                     
CTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTA  >  Exported/385820‑385959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: