Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,895 T→C 12.4% L369L (TTA→CTA) ‡ cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,8950TC12.4% 285.0 / 25.5 113L369L (TTA→CTA) ‡cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base T (41/58);  new base C (9/5);  total (50/63)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

Only 100 of 127 total aligned reads displayed.

CTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGC  >  Exported/385825‑385965
                                                                      |                                                                      
ctctTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGt                                                                        >  1:1357813/1‑71 (MQ=255)
  cttctGCGACCACGCCGAAGATGCTGGAGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGt                                                                        >  1:2410232/1‑69 (MQ=255)
   ttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTaa                                                                     >  1:25075/1‑71 (MQ=255)
    tctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGtt                                                                       <  1:2364841/68‑1 (MQ=255)
     ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa                                                                      <  1:2760664/68‑1 (MQ=255)
     ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa                                                                      <  1:3034470/68‑1 (MQ=255)
     ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa                                                                      <  1:4899299/68‑1 (MQ=255)
     ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa                                                                      <  1:4011975/68‑1 (MQ=255)
     ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa                                                                      <  1:2013463/68‑1 (MQ=255)
     ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAAt                                                                    >  1:1746415/1‑70 (MQ=255)
        ggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg                                                                  <  1:5649198/69‑1 (MQ=255)
        ggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg                                                                  <  1:2247715/69‑1 (MQ=255)
         gACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAAt                                                               <  1:433986/71‑1 (MQ=255)
         gACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAAt                                                               <  1:440833/71‑1 (MQ=255)
           ccACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATcc                                                             <  1:4484983/71‑1 (MQ=255)
              caccGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTGATGGAATCCtt                                                           <  1:409881/68‑1 (MQ=255)
              caccGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTGATGGAATCCtt                                                           <  1:1252535/68‑1 (MQ=255)
              caccGAAGATGCTGGCGAACGAAGGGCAGACGTGCTTTATCGGCTACGGTGGGATGTTGATGGAATCCtt                                                           <  1:5587177/68‑1 (MQ=255)
              cGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTc                                                          <  1:1682076/71‑1 (MQ=255)
               gCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCg                                                         >  1:5626112/1‑71 (MQ=255)
                ccGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATGCTTCGt                                                        <  1:6528/71‑1 (MQ=255)
                ccGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGt                                                        <  1:4641613/71‑1 (MQ=255)
                  gAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATcc                                                             >  1:4508440/1‑64 (MQ=255)
                       tGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTaa                                                                     >  1:4227598/1‑51 (MQ=255)
                       tGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTaa                                                                     >  1:3732506/1‑51 (MQ=255)
                       tGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTaa                                                                     >  1:5374554/1‑51 (MQ=255)
                        gCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATcc                                                             >  1:3680521/1‑58 (MQ=255)
                        gCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                                >  1:1189088/1‑71 (MQ=255)
                        gCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                                >  1:3148184/1‑71 (MQ=255)
                        gCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                                >  1:2766293/1‑71 (MQ=255)
                        gCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                                >  1:2123505/1‑71 (MQ=255)
                            gCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTGATGGAATCCtt                                                           <  1:935580/56‑1 (MQ=255)
                            gCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGgc                                            >  1:789094/1‑71 (MQ=255)
                            gCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGgc                                            <  1:746920/71‑1 (MQ=255)
                              gaacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTGATGGAATCCtt                                                           <  1:2906134/54‑1 (MQ=255)
                               aacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGtt                                                                       <  1:2094552/41‑1 (MQ=255)
                               aacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGtt                                                                       <  1:1339751/41‑1 (MQ=255)
                               aacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGtt                                                                       <  1:2114374/41‑1 (MQ=255)
                               aacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGtt                                                                       <  1:1505732/41‑1 (MQ=255)
                               aacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCt                                         <  1:2447684/71‑1 (MQ=255)
                                 cgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAAt                                                               >  1:4405294/1‑47 (MQ=255)
                                 cgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATc                                                              >  1:3750771/1‑48 (MQ=255)
                                  gaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGa                                                   >  1:620664/1‑58 (MQ=255)
                                  gaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGa                                                   >  1:5062498/1‑58 (MQ=255)
                                  gaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGt                                      <  1:1472331/71‑1 (MQ=255)
                                   aaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGtt                                     >  1:4509184/1‑71 (MQ=255)
                                   aaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGtt                                     <  1:4389761/71‑1 (MQ=255)
                                        cAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGc                                >  1:4717433/1‑71 (MQ=255)
                                           gCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTg                             <  1:5210306/71‑1 (MQ=255)
                                            agTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGt                            <  1:171864/70‑1 (MQ=255)
                                              tGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCtt                                                           <  1:4061539/38‑1 (MQ=255)
                                              tGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCtt                                                           <  1:3634666/38‑1 (MQ=255)
                                              tGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCtt                                                           <  1:5316669/38‑1 (MQ=255)
                                              tGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTg                                                       <  1:63155/42‑1 (MQ=255)
                                              tGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTat                          >  1:5383460/1‑71 (MQ=255)
                                                cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatca                        >  1:796552/1‑71 (MQ=255)
                                                cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatca                        <  1:2988911/71‑1 (MQ=255)
                                                cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatc                         <  1:382975/70‑1 (MQ=255)
                                                cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatc                         <  1:2660878/70‑1 (MQ=255)
                                                 tttATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTAt                                               <  1:1986052/47‑1 (MQ=255)
                                                  tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTa                                                >  1:4385773/3‑45 (MQ=255)
                                                  tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                      >  1:5459176/3‑71 (MQ=255)
                                                  tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                      >  1:4048213/3‑71 (MQ=255)
                                                  tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                      >  1:1024988/3‑71 (MQ=255)
                                                   tATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGAt                                                  <  1:4782108/42‑1 (MQ=255)
                                                   tATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTcc                                  >  1:5249769/1‑58 (MQ=255)
                                                   caTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                      >  1:2165164/2‑70 (MQ=255)
                                                    aTCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                                >  1:2587767/1‑43 (MQ=255)
                                                    aTCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                                >  1:400964/1‑43 (MQ=255)
                                                    aTCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCg                     <  1:2290757/70‑1 (MQ=255)
                                                     tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGt                            >  1:3036305/1‑62 (MQ=255)
                                                     tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGt                            >  1:3774717/1‑62 (MQ=255)
                                                     tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatca                        <  1:43401/66‑1 (MQ=255)
                                                     tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa                    <  1:5618758/70‑1 (MQ=255)
                                                     tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa                    <  1:342877/70‑1 (MQ=255)
                                                     tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa                    <  1:3811882/70‑1 (MQ=255)
                                                      cGGCTACGGGGGGATGTTAATGGAATCCTTCGTGGCGAt                                                  <  1:2418344/39‑1 (MQ=255)
                                                       ggCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatcatc                      >  1:3924332/1‑66 (MQ=255)
                                                       ggCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatcatc                      >  1:1404056/1‑66 (MQ=255)
                                                       ggCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGaac                  <  1:401342/70‑3 (MQ=255)
                                                       ggCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAAcc                 <  1:1386348/71‑1 (MQ=255)
                                                       ggCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAAcc                 >  1:3009516/1‑71 (MQ=255)
                                                        gCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCg                <  1:1752776/71‑1 (MQ=255)
                                                            cGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCggg              <  1:1775997/69‑1 (MQ=255)
                                                               tGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTg                             <  1:987350/51‑1 (MQ=255)
                                                                gggATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTa        >  1:4061144/1‑71 (MQ=255)
                                                                  gATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatcatc                      <  1:2527693/55‑1 (MQ=255)
                                                                  gATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAtt      <  1:3964663/71‑1 (MQ=255)
                                                                   aTGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATGGATCCGGGCGTGTAttt     >  1:319888/1‑71 (MQ=255)
                                                                   aTGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt     >  1:1417581/1‑71 (MQ=255)
                                                                    tGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGt                                      <  1:1261406/37‑1 (MQ=255)
                                                                     gTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTc                                   <  1:2484157/39‑1 (MQ=255)
                                                                     gTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTc                                   <  1:560446/39‑1 (MQ=255)
                                                                     gTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTc                                   <  1:987297/39‑1 (MQ=255)
                                                                     gTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTg   >  1:1918695/1‑71 (MQ=255)
                                                                      ttAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa                    <  1:782660/53‑1 (MQ=255)
                                                                      ttAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa                    <  1:229017/53‑1 (MQ=255)
                                                                      ttAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa                    <  1:2767376/53‑1 (MQ=255)
                                                                      ttAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGAt                   >  1:3080136/1‑54 (MQ=255)
                                                                      ttAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGc  >  1:3382033/1‑71 (MQ=255)
                                                                      |                                                                      
CTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGC  >  Exported/385825‑385965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: