Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,903 A→G 10.9% E371E (GAA→GAG cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,9030AG10.9% 336.3 / 25.0 128E371E (GAA→GAGcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base A (50/64);  new base G (9/5);  total (59/69)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

Only 100 of 129 total aligned reads displayed.

GACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGA  >  Exported/385834‑385970
                                                                     |                                                                   
gACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAAt                                                                    <  1:433986/71‑1 (MQ=255)
     caccGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTGATGGAATCCtt                                                                <  1:1252535/68‑1 (MQ=255)
     caccGAAGATGCTGGCGAACGAAGGGCAGACGTGCTTTATCGGCTACGGTGGGATGTTGATGGAATCCtt                                                                <  1:5587177/68‑1 (MQ=255)
     cGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTc                                                               >  1:3637276/1‑71 (MQ=255)
     cGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTc                                                               <  1:1682076/71‑1 (MQ=255)
      gCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCg                                                              <  1:898131/71‑1 (MQ=255)
      gCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCg                                                              >  1:5626112/1‑71 (MQ=255)
       ccGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGt                                                             <  1:4641613/71‑1 (MQ=255)
         gAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATcc                                                                  >  1:4508440/1‑64 (MQ=255)
               gCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATcc                                                                  >  1:3680521/1‑58 (MQ=255)
               gCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                                     >  1:1189088/1‑71 (MQ=255)
               gCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                                     >  1:3148184/1‑71 (MQ=255)
               gCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                                     >  1:2766293/1‑71 (MQ=255)
               gCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                                     >  1:2123505/1‑71 (MQ=255)
                   gCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGgc                                                 >  1:789094/1‑71 (MQ=255)
                   gCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGgc                                                 <  1:746920/71‑1 (MQ=255)
                     gaacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTGATGGAATCCtt                                                                <  1:2906134/54‑1 (MQ=255)
                      aacgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCt                                              <  1:2447684/71‑1 (MQ=255)
                       acgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATTGAATCCTTCGTGGCGATTATGGCGCTg                                             >  1:2246043/1‑71 (MQ=255)
                        cgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAAt                                                                    >  1:4405294/1‑47 (MQ=255)
                        cgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAAt                                                                    >  1:4450174/1‑47 (MQ=255)
                        cgaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATc                                                                   >  1:3750771/1‑48 (MQ=255)
                         gaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGa                                                        >  1:620664/1‑58 (MQ=255)
                         gaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGt                                           <  1:1472331/71‑1 (MQ=255)
                         gaaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGt                                           <  1:4094316/71‑1 (MQ=255)
                          aaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGtt                                          <  1:4389761/71‑1 (MQ=255)
                          aaGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGtt                                          >  1:4509184/1‑71 (MQ=255)
                             ggCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTcc                                       <  1:1768261/71‑1 (MQ=255)
                               cAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGc                                     >  1:4717433/1‑71 (MQ=255)
                                  gCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTg                                  <  1:5210306/71‑1 (MQ=255)
                                     tGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCtt                                                                <  1:5316669/38‑1 (MQ=255)
                                     tGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCtt                                                                <  1:4061539/38‑1 (MQ=255)
                                     tGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTg                                                            <  1:63155/42‑1 (MQ=255)
                                     tGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTat                               >  1:5383460/1‑71 (MQ=255)
                                       cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGa                                                        >  1:1709021/1‑44 (MQ=255)
                                       cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatca                             <  1:2988911/71‑1 (MQ=255)
                                       cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatca                             >  1:796552/1‑71 (MQ=255)
                                       cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatc                              <  1:2660878/70‑1 (MQ=255)
                                       cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatc                              <  1:382975/70‑1 (MQ=255)
                                        tttATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTAt                                                    <  1:1986052/47‑1 (MQ=255)
                                         tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTa                                                     >  1:4385773/3‑45 (MQ=255)
                                         tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                           >  1:4048213/3‑71 (MQ=255)
                                         tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                           >  1:1024988/3‑71 (MQ=255)
                                         tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                           >  1:2478888/3‑71 (MQ=255)
                                         tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                           >  1:5459176/3‑71 (MQ=255)
                                         tcaTCGGCTACGGAGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                           >  1:1412700/3‑71 (MQ=255)
                                          tATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGAt                                                       <  1:4782108/42‑1 (MQ=255)
                                          tATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGAt                                                       <  1:714986/42‑1 (MQ=255)
                                          tATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTcc                                       >  1:5249769/1‑58 (MQ=255)
                                           aTCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                                     >  1:400964/1‑43 (MQ=255)
                                           aTCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTa                                                     >  1:2587767/1‑43 (MQ=255)
                                           aTCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCg                          <  1:2290757/70‑1 (MQ=255)
                                           aTCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCg                          <  1:2067750/70‑1 (MQ=255)
                                            tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGt                                 >  1:3774717/1‑62 (MQ=255)
                                            tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa                         <  1:342877/70‑1 (MQ=255)
                                            tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa                         <  1:5618758/70‑1 (MQ=255)
                                            tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa                         <  1:564175/70‑1 (MQ=255)
                                            tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa                         <  1:3811882/70‑1 (MQ=255)
                                            tCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGaa                        >  1:4965950/1‑70 (MQ=255)
                                             cGGCTACGGGGGGATGTTAATGGAATCCTTCGTGGCGAt                                                       <  1:2418344/39‑1 (MQ=255)
                                             cGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGAt                                                       <  1:5372381/39‑1 (MQ=12)
                                              ggCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatcatc                           >  1:4375191/1‑66 (MQ=255)
                                              ggCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatcatc                           >  1:1404056/1‑66 (MQ=255)
                                              ggCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatcatc                           >  1:3924332/1‑66 (MQ=255)
                                              ggCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGaac                       <  1:401342/70‑3 (MQ=255)
                                              ggCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAAcc                      <  1:1386348/71‑1 (MQ=255)
                                              ggCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAAcc                      >  1:3009516/1‑71 (MQ=255)
                                               gCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCg                     <  1:1752776/71‑1 (MQ=255)
                                                   cGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCggg                   <  1:1775997/69‑1 (MQ=255)
                                                      tGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTg                                  <  1:987350/51‑1 (MQ=255)
                                                       gggATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTa             >  1:4061144/1‑71 (MQ=255)
                                                         gATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatcatc                           <  1:2527693/55‑1 (MQ=255)
                                                         gATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAtt           <  1:3964663/71‑1 (MQ=255)
                                                          aTGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATGGATCCGGGCGTGTAttt          >  1:319888/1‑71 (MQ=255)
                                                            gTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTc                                        <  1:987297/39‑1 (MQ=255)
                                                            gTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTc                                        <  1:560446/39‑1 (MQ=255)
                                                             ttAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa                         <  1:2767376/53‑1 (MQ=255)
                                                             ttAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa                         <  1:229017/53‑1 (MQ=255)
                                                             ttAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGAt                        >  1:3080136/1‑54 (MQ=255)
                                                             ttAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGc       >  1:3382033/1‑71 (MQ=255)
                                                              tAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatc                              >  1:536425/1‑47 (MQ=255)
                                                              tAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatc                              >  1:1563734/1‑47 (MQ=255)
                                                               aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt          >  1:957108/1‑66 (MQ=255)
                                                               aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCa     >  1:2477081/1‑71 (MQ=255)
                                                               aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCa     >  1:442668/1‑71 (MQ=255)
                                                               aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCa     >  1:4313481/1‑71 (MQ=255)
                                                                aTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCa     >  1:2778469/1‑70 (MQ=255)
                                                                aTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCAt    <  1:3215696/71‑1 (MQ=255)
                                                                aTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCAt    <  1:4172047/71‑1 (MQ=255)
                                                                 tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTa                                <  1:5695117/42‑1 (MQ=255)
                                                                 tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTa                                <  1:690437/42‑1 (MQ=255)
                                                                 tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTa                                <  1:1225190/42‑1 (MQ=255)
                                                                 tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTa                                <  1:2489596/42‑1 (MQ=255)
                                                                 tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt          >  1:4449895/1‑64 (MQ=255)
                                                                 tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg   <  1:3588898/71‑1 (MQ=255)
                                                                 tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg   <  1:5561103/71‑1 (MQ=255)
                                                                 tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg   <  1:5190215/71‑1 (MQ=255)
                                                                  ggAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTCTATTTTGCCATg   >  1:5022132/1‑70 (MQ=255)
                                                                   gAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGa  <  1:1944747/70‑1 (MQ=255)
                                                                     aTCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTg                                  <  1:696274/36‑1 (MQ=255)
                                                                     |                                                                   
GACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGA  >  Exported/385834‑385970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: