Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	132,257	C→G	13.0%	Q184E (CAG→GAG) ‡	yadD →	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	132,257	0	C	G	13.0%	237.4 / 23.4	92	Q184E (CAG→GAG) ‡	yadD	predicted transposase
Reads supporting (aligned to +/- strand):  ref base C (48/32);  new base G (5/7);  total (53/39)
Fisher's exact test for biased strand distribution p-value = 3.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

ACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGTCA  >  Exported/132188‑132327                                                                      |                                                                       acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc                                                                        <  1:5225852/70‑1 (MQ=255) acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc                                                                        <  1:1456262/70‑1 (MQ=255)     cGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcgc                                                                   <  1:1982600/71‑1 (MQ=255)     cGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcgc                                                                   >  1:5603944/1‑71 (MQ=255)     cGGATGACGAAATCATGCAACATAGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcg                                                                    >  1:4314735/1‑70 (MQ=255)         tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACt                                                               <  1:5105414/71‑1 (MQ=255)           aCGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACtt                                                              >  1:4311932/1‑70 (MQ=255)              agaTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTAAtc                                                          >  1:5264174/3‑70 (MQ=255)               aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                                                         <  1:4346016/71‑1 (MQ=255)                aTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACcgcg                                                                  <  1:1981471/61‑1 (MQ=255)                aTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTAAtcgg                                                        <  1:3144933/71‑4 (MQ=255)                 tCATGCAACATCGGCGTATAGCTCTGCTTGAACTGATTCAAAAGCATATTCGCGACCGCGat                                                                <  1:437409/62‑2 (MQ=255)                 tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                                                                <  1:5381492/62‑2 (MQ=255)                 tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                                                                <  1:3389478/62‑2 (MQ=255)                 tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGAttt                                                              <  1:4614380/64‑1 (MQ=255)                 tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTAAtcgg                                                        <  1:4086207/70‑4 (MQ=255)                  cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                                                                >  1:2371657/1‑60 (MQ=255)                  cATGCAACATAGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                                                                >  1:3248330/1‑60 (MQ=255)                      cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcg                                                                    >  1:1678405/1‑53 (MQ=255)                      cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt                                                  >  1:121453/1‑71 (MQ=255)                      cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt                                                  <  1:4014833/71‑1 (MQ=255)                      cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt                                                  >  1:1755295/1‑71 (MQ=255)                       aaCATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                                                             >  1:2398642/1‑59 (MQ=255)                       aaCATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                                                             >  1:2550432/1‑59 (MQ=255)                        aCATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcgc                                                                   <  1:825848/52‑1 (MQ=255)                          aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                                                            >  1:5389881/1‑57 (MQ=255)                          aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                                                            >  1:4756399/1‑57 (MQ=255)                          aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg                                              >  1:3152257/1‑71 (MQ=255)                            cggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAAt                                                           >  1:4727016/1‑56 (MQ=255)                            cggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAAt                                                           >  1:4733468/1‑56 (MQ=255)                            cggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAAt                                                           >  1:5388259/1‑56 (MQ=255)                             ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                                           >  1:5025523/1‑71 (MQ=255)                             ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                                           <  1:1932043/71‑1 (MQ=255)                             ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                                           >  1:549698/1‑71 (MQ=255)                             ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                                           <  1:1726524/71‑1 (MQ=255)                                ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACt                                         <  1:1854585/70‑1 (MQ=255)                                       gATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt                                                 <  1:1957148/55‑1 (MQ=255)                                       gATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt                                                 <  1:1875035/55‑1 (MQ=255)                                         ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCt                                >  1:5450095/1‑70 (MQ=255)                                         ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTg                               <  1:2192961/71‑1 (MQ=255)                                          tctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGa                              >  1:1205416/1‑71 (MQ=255)                                          tctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGa                              >  1:3072992/1‑71 (MQ=255)                                           ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                                                            >  1:1582883/1‑40 (MQ=255)                                           ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                                           >  1:3476834/1‑57 (MQ=255)                                           ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                                           >  1:4439828/1‑57 (MQ=255)                                              gAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTAt                                                      >  1:1217928/1‑43 (MQ=255)                                              gAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAt                             <  1:498423/68‑1 (MQ=255)                                              gAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATc                            <  1:2636217/69‑1 (MQ=255)                                                actactGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgcc                         >  1:4945734/1‑68 (MQ=255)                                                actactGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgac                         <  1:27477/70‑1 (MQ=255)                                                actactGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgac                         >  1:4301254/1‑70 (MQ=255)                                                   actGCAAAAACATATTCGCCAGCGCGACTTTATGt                                                         <  1:2813211/35‑1 (MQ=255)                                                   actGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAg                     >  1:4110214/1‑71 (MQ=255)                                                    ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAAc                                          >  1:820289/1‑49 (MQ=255)                                                    ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAAc                                          >  1:2780334/1‑49 (MQ=255)                                                    ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATc                            <  1:1448313/63‑1 (MQ=255)                                                    ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGaa                      >  1:723550/1‑69 (MQ=255)                                                    ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAgg                    >  1:3767974/1‑71 (MQ=255)                                                     tGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGc                                             <  1:5630840/45‑1 (MQ=255)                                                     tGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGt                                      <  1:2358735/52‑1 (MQ=255)                                                      gCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgacga                       >  1:4396399/1‑66 (MQ=255)                                                      gCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgacga                       >  1:5393177/1‑66 (MQ=255)                                                       cAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAt                             >  1:748620/1‑59 (MQ=255)                                                       cAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAt                             >  1:3749686/1‑59 (MQ=255)                                                       cAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAt                             >  1:671609/1‑59 (MQ=255)                                                       cAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAt                             >  1:3560665/1‑59 (MQ=255)                                                       cAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcga                          >  1:692509/1‑62 (MQ=255)                                                       cAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAggg                   <  1:4352719/69‑1 (MQ=255)                                                        aaaaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAg                     >  1:4778198/1‑66 (MQ=255)                                                        aaaaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTac                <  1:3883655/71‑1 (MQ=255)                                                        aaaaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTa                 <  1:3334884/70‑1 (MQ=255)                                                        aaaaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTa                 <  1:3088165/70‑1 (MQ=255)                                                        aaaaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCAACGAAg                     >  1:3339152/1‑66 (MQ=255)                                                          aaaCATATTCGCCAGCGCGACTTAATGTTATTGCtt                                                 <  1:1658124/36‑1 (MQ=255)                                                          aaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcg                           <  1:279077/58‑1 (MQ=255)                                                          aaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTacac              >  1:4318894/1‑71 (MQ=255)                                                            aCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTacac              >  1:2959748/1‑69 (MQ=255)                                                            aCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTacac              >  1:1876175/1‑69 (MQ=255)                                                            aCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTacac              >  1:542714/1‑69 (MQ=255)                                                            aCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTa            <  1:3289267/71‑1 (MQ=255)                                                               tatTCGCCAGCGCGACTTAATGTTATTGCTTGAGc                                             <  1:1829889/35‑1 (MQ=255)                                                               tatTCGCCAGCGCGACTTAATGTTATTGCTTGAGc                                             <  1:1573545/35‑1 (MQ=255)                                                               tatTCGCCAGCGCGACTTAATGTTATTGCTTGAGc                                             <  1:927884/35‑1 (MQ=255)                                                               tatTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCg         <  1:1865938/71‑1 (MQ=255)                                                                 ttCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGc          >  1:3016066/1‑68 (MQ=255)                                                                 ttCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGc          >  1:4745196/1‑68 (MQ=255)                                                                 ttCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCgg        <  1:2048286/70‑1 (MQ=255)                                                                  tCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCgg        >  1:3781331/1‑69 (MQ=255)                                                                   cGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAg     >  1:631586/1‑71 (MQ=255)                                                                   cGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAg     >  1:1088716/1‑71 (MQ=255)                                                                    gCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAg                     >  1:661355/1‑54 (MQ=255)                                                                    gCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAg                     >  1:4285262/1‑54 (MQ=255)                                                                    gCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGt    <  1:2896582/71‑1 (MQ=255)                                                                    gCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGt    <  1:4878921/71‑1 (MQ=255)                                                                      cAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGTCa  <  1:5001049/71‑1 (MQ=255)                                                                      |                                                                       ACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGTCA  >  Exported/132188‑132327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑