| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 385,948 | T→A | 24.9% | D386E (GAT→GAA) | cstA → | carbon starvation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 385,948 | 0 | T | A | 24.9% | 93.9 / 28.2 | 52 | D386E (GAT→GAA) | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base T (20/18); new base A (7/6); total (27/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCG > Exported/385880‑386018 | ggCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAAcc < 1:1386348/71‑1 (MQ=255)ggCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAAcc > 1:3009516/1‑71 (MQ=255) gCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCg < 1:396407/71‑1 (MQ=255) gCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCg < 1:1752776/71‑1 (MQ=255) cGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCggg < 1:1775997/69‑1 (MQ=255) gggATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTa > 1:4061144/1‑71 (MQ=255) gATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAtt < 1:3964663/71‑1 (MQ=255) aTGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATGGATCCGGGCGTGTAttt > 1:319888/1‑71 (MQ=255) aTGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt > 1:1417581/1‑71 (MQ=255) gTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTg > 1:1918695/1‑71 (MQ=255) ttAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGAt > 1:3080136/1‑54 (MQ=255) ttAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGc > 1:3382033/1‑71 (MQ=255) aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGc > 1:5671578/1‑58 (MQ=255) aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGc > 1:5313509/1‑58 (MQ=255) aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt > 1:957108/1‑66 (MQ=255) aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGc > 1:3199603/1‑69 (MQ=255) aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCa > 1:2477081/1‑71 (MQ=255) aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCa > 1:442668/1‑71 (MQ=255) aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCa > 1:4313481/1‑71 (MQ=255) aTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCa > 1:2778469/1‑70 (MQ=255) aTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCAt < 1:3215696/71‑1 (MQ=255) aTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCAt < 1:4172047/71‑1 (MQ=255) tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt > 1:4449895/1‑64 (MQ=255) tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg < 1:3588898/71‑1 (MQ=255) tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg < 1:5561103/71‑1 (MQ=255) tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg < 1:5190215/71‑1 (MQ=255) tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg < 1:4695503/71‑1 (MQ=255) ggAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTCTATTTTGCCATg > 1:5022132/1‑70 (MQ=255) gAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGa < 1:1944747/70‑1 (MQ=255) tCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACac < 1:1128206/71‑2 (MQ=255) cTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGcc > 1:144419/1‑71 (MQ=255) cTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACAccc > 1:5577617/1‑71 (MQ=255) tCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCg > 1:1968933/1‑71 (MQ=255) cGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGAt > 1:3225489/1‑40 (MQ=255) cGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGAt > 1:3701285/1‑40 (MQ=255) cGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGAt > 1:1710372/1‑40 (MQ=255) cGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGAt > 1:5564122/1‑40 (MQ=255) cGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGAt > 1:2813461/1‑40 (MQ=255) cGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc < 1:5523451/69‑1 (MQ=255) cGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc > 1:4800594/1‑69 (MQ=255) cGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCgc > 1:1954198/1‑70 (MQ=255) cGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCgc > 1:2396515/1‑70 (MQ=255) cGTGGCGATTACGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc < 1:1744653/69‑1 (MQ=255) gCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCgc < 1:1170488/67‑2 (MQ=255) gCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCgc < 1:1177082/67‑2 (MQ=255) aTTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGt < 1:5356536/71‑1 (MQ=255) tATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACAccc > 1:122627/1‑59 (MQ=255) tATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACAccc > 1:2232338/1‑59 (MQ=255) tGGCGCTGGTTTCCGCCTGTATCATCGAGCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCt < 1:2370925/70‑1 (MQ=255) cTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATg > 1:4033427/1‑57 (MQ=255) ggTTTCCGCCTGTATCATCGATCCTGGCGTGTATTTTGCCATGAACAGccc < 1:46340/51‑1 (MQ=255) ggTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc < 1:1721911/51‑1 (MQ=255) tCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg < 1:5483642/39‑1 (MQ=255) tCCGCCTGTATCATCGATCCGGGCGTGTATTTAGCCATg < 1:1252400/39‑1 (MQ=255) ccTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGAcg > 1:3031944/1‑71 (MQ=255) tATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATg < 1:764686/43‑1 (MQ=255) atcatcGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATg < 1:3170218/42‑1 (MQ=255) tcatcGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGAt > 1:3087739/1‑71 (MQ=255) tCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCg < 1:3520844/71‑1 (MQ=255) tCCGGGCGTGTATTTTACCATTAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCg < 1:1715959/71‑1 (MQ=255) | GGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCG > Exported/385880‑386018 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |