Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 385,960 | T→C | 18.3% | Y390Y (TAT→TAC) | cstA → | carbon starvation protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 385,960 | 0 | T | C | 18.3% | 100.6 / 15.7 | 49 | Y390Y (TAT→TAC) | cstA | carbon starvation protein |
Reads supporting (aligned to +/- strand): ref base T (16/24); new base C (6/3); total (22/27) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.66e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 |
GATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGC > Exported/385891‑386030 | gATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAtt < 1:3964663/71‑1 (MQ=255) aTGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATGGATCCGGGCGTGTAttt > 1:319888/1‑71 (MQ=255) aTGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt > 1:1417581/1‑71 (MQ=255) gTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTg > 1:1918695/1‑71 (MQ=255) ttAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGc > 1:3382033/1‑71 (MQ=255) aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt > 1:957108/1‑66 (MQ=255) aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGc > 1:3199603/1‑69 (MQ=255) aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCa > 1:4313481/1‑71 (MQ=255) aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCa > 1:442668/1‑71 (MQ=255) aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCa > 1:2477081/1‑71 (MQ=255) aTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCa > 1:2778469/1‑70 (MQ=255) aTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCAt < 1:3215696/71‑1 (MQ=255) aTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCAt < 1:4172047/71‑1 (MQ=255) tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt > 1:4449895/1‑64 (MQ=255) tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg < 1:5561103/71‑1 (MQ=255) tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg < 1:5190215/71‑1 (MQ=255) tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg < 1:3588898/71‑1 (MQ=255) tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg < 1:4695503/71‑1 (MQ=255) ggAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTCTATTTTGCCATg > 1:5022132/1‑70 (MQ=255) gAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGa < 1:1944747/70‑1 (MQ=255) tCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACac < 1:1128206/71‑2 (MQ=255) cTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGcc > 1:144419/1‑71 (MQ=255) cTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACAccc > 1:5577617/1‑71 (MQ=255) tCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCg > 1:1968933/1‑71 (MQ=255) cGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc > 1:4800594/1‑69 (MQ=255) cGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc < 1:5523451/69‑1 (MQ=255) cGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCgc > 1:2396515/1‑70 (MQ=255) cGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCgc > 1:1954198/1‑70 (MQ=255) cGTGGCGATTACGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc < 1:1744653/69‑1 (MQ=255) gCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCgc < 1:1170488/67‑2 (MQ=255) gCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCgc < 1:1177082/67‑2 (MQ=255) aTTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGt < 1:5356536/71‑1 (MQ=255) tATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACAccc > 1:122627/1‑59 (MQ=255) tATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACAccc > 1:2232338/1‑59 (MQ=255) tGGCGCTGGTTTCCGCCTGTATCATCGAGCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCt < 1:2370925/70‑1 (MQ=255) cTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATg > 1:4033427/1‑57 (MQ=255) ggTTTCCGCCTGTATCATCGATCCTGGCGTGTATTTTGCCATGAACAGccc < 1:46340/51‑1 (MQ=255) ggTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc < 1:1721911/51‑1 (MQ=255) tCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg < 1:5483642/39‑1 (MQ=255) tCCGCCTGTATCATCGATCCGGGCGTGTATTTAGCCATg < 1:1252400/39‑1 (MQ=255) ccTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGAcg > 1:3031944/1‑71 (MQ=255) tATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATg < 1:764686/43‑1 (MQ=255) atcatcGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATg < 1:3170218/42‑1 (MQ=255) tcatcGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGAt > 1:3087739/1‑71 (MQ=255) tCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCg < 1:3520844/71‑1 (MQ=255) tCCGGGCGTGTATTTTACCATTAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCg < 1:1715959/71‑1 (MQ=255) ccGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGc > 1:5260507/1‑71 (MQ=255) cGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCt < 1:4650254/71‑1 (MQ=255) ggCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTc < 1:336755/71‑1 (MQ=255) gCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCt < 1:3744493/71‑1 (MQ=255) cGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTg < 1:3751628/71‑1 (MQ=255) cGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTg < 1:48219/71‑1 (MQ=255) tATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCcgc > 1:3079273/1‑71 (MQ=255) tATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCcg > 1:963691/1‑70 (MQ=255) ttttGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGTCGGATGTGGTCGCTTCTGCcgcg < 1:1938472/70‑1 (MQ=255) ttttGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGc < 1:2033383/60‑1 (MQ=255) ttttGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCcgcg > 1:19775/1‑70 (MQ=255) ttttGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCcgcg < 1:4090815/70‑1 (MQ=255) ttttGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCcgcg < 1:365381/70‑1 (MQ=255) ttttGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCgcgc < 1:5174180/71‑1 (MQ=255) | GATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGC > Exported/385891‑386030 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |