Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 386,245 G→C 8.7% T485T (ACG→ACC cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported386,2450GC 8.7% 232.4 / 9.7 80T485T (ACG→ACCcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (33/40);  new base C (3/4);  total (36/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

GGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTCCTGGCC  >  Exported/386176‑386315
                                                                     |                                                                      
gggCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGAcgg                                                                       >  1:3454238/1‑71 (MQ=255)
       atgatgGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGa                                                                >  1:3274472/1‑71 (MQ=255)
       atgatgGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGa                                                                >  1:1404732/1‑71 (MQ=255)
          atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGAcggcgg                                                                    >  1:1608238/1‑64 (MQ=255)
          atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATg                                                              >  1:5325828/1‑70 (MQ=255)
          atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATg                                                              >  1:5324654/1‑70 (MQ=255)
          atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATg                                                              <  1:1738957/70‑1 (MQ=255)
          atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGc                                                             <  1:4526423/71‑1 (MQ=255)
          atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGc                                                             <  1:3084162/71‑1 (MQ=255)
          atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGc                                                             <  1:4406319/71‑1 (MQ=255)
             gatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGa                                                                >  1:4132793/1‑65 (MQ=255)
             gatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAgg                                                          <  1:1297316/71‑1 (MQ=255)
              atgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGTTGCAGGt                                                         <  1:33828/71‑1 (MQ=255)
              atgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAgg                                                          >  1:5211871/1‑70 (MQ=255)
                   gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg                                                    <  1:3166406/71‑1 (MQ=255)
                   gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg                                                    <  1:2668341/71‑1 (MQ=255)
                   gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg                                                    <  1:293709/71‑1 (MQ=255)
                   gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg                                                    <  1:806370/71‑1 (MQ=255)
                   gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg                                                    <  1:5476324/71‑1 (MQ=255)
                   gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgc                                                     <  1:4521844/70‑1 (MQ=255)
                    gcttCTGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGTAcccg                                                    >  1:3733837/3‑70 (MQ=255)
                    gcttCTGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGTACCCGt                                                   <  1:305927/69‑1 (MQ=255)
                    gcttCTGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGTACCCGt                                                   <  1:3008848/69‑1 (MQ=255)
                    gcgtCTGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGt                                                         <  1:5138441/62‑1 (MQ=255)
                    cGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg                                                    <  1:3595086/70‑1 (MQ=255)
                    cGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGt                                                   <  1:5282299/71‑1 (MQ=255)
                    cGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGt                                                   <  1:3870259/71‑1 (MQ=255)
                    cGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGt                                                   <  1:4382262/71‑1 (MQ=255)
                     gTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtg                                                  <  1:3025731/71‑1 (MQ=255)
                     gTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtg                                                  <  1:731110/71‑1 (MQ=255)
                      ttCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGc                                                             <  1:3724063/59‑1 (MQ=255)
                      ttCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgc                                                 <  1:472205/71‑1 (MQ=255)
                        cTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGAcgg                                                                       >  1:558162/1‑47 (MQ=255)
                        cTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCa                                                            >  1:5628888/1‑58 (MQ=255)
                        cTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgct                                                <  1:330016/70‑1 (MQ=255)
                         tGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGc                                                             <  1:3908714/56‑1 (MQ=255)
                         tGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGTACCCGttctgg                                              >  1:4394724/1‑70 (MQ=255)
                         tGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGTACCCGttctgg                                              >  1:3426748/1‑70 (MQ=255)
                             aTCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTgcgcgc                                          >  1:2687181/1‑71 (MQ=255)
                             aTCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTgcg                                             >  1:5136372/1‑68 (MQ=255)
                             aTCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTgcg                                             >  1:1395470/1‑68 (MQ=255)
                               cACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAg                                                           >  1:5262176/1‑52 (MQ=255)
                               cACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAg                                                           >  1:3783402/1‑52 (MQ=255)
                                aCTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCtt                                        >  1:627540/1‑70 (MQ=255)
                                  ttCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcg                                                      <  1:4238556/54‑1 (MQ=255)
                                  ttCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcg                                                      <  1:714393/54‑1 (MQ=255)
                                   tCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATg                                    >  1:1132488/1‑71 (MQ=255)
                                    cGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTAt                                     <  1:5523042/69‑1 (MQ=255)
                                     gCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgct                                                >  1:3073553/1‑57 (MQ=255)
                                     gCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgct                                                >  1:2355952/1‑57 (MQ=255)
                                     gCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgct                                                >  1:1514813/1‑57 (MQ=255)
                                      ccATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTg                                 <  1:5128654/71‑1 (MQ=255)
                                       cATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGc                                <  1:1934363/71‑1 (MQ=255)
                                       cATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGc                                <  1:444402/71‑1 (MQ=255)
                                         ttttGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAg                              <  1:3063722/71‑1 (MQ=255)
                                          tttgtttgAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAg                              >  1:1372550/1‑70 (MQ=255)
                                           ttgtttgAAGCACTGTTTATTCTGACGGCGGTGGa                                                                >  1:5313221/1‑35 (MQ=255)
                                           ttgtttgAAGCACTGTTTATTCTGACGGCGGTGGa                                                                >  1:1346262/1‑35 (MQ=255)
                                           ttgtttgAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTa                                                        >  1:4652966/1‑43 (MQ=255)
                                           ttgtttgAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgctgc                                              >  1:5369582/1‑53 (MQ=255)
                                           ttgtttgAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTAt                                     >  1:671389/1‑62 (MQ=255)
                                                 gAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTgcgc                                            <  1:4928386/49‑1 (MQ=255)
                                                 gAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTgcgc                                            <  1:4128685/49‑1 (MQ=255)
                                                 gAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTa                                      <  1:181016/55‑1 (MQ=255)
                                                 gAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATctgc                       <  1:2314553/70‑1 (MQ=255)
                                                 gAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGATGCGCGCTTTa                                      <  1:927550/55‑1 (MQ=255)
                                                 gAAGCACTGTTTATTTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTa                                      <  1:1710832/54‑1 (MQ=255)
                                                  aaGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATctgctg                     <  1:4754531/71‑1 (MQ=255)
                                                  aaGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATctgct                      <  1:1764216/70‑1 (MQ=255)
                                                        tGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtg                                                  <  1:5249229/36‑1 (MQ=255)
                                                         gTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgctg                                               >  1:1157827/1‑38 (MQ=255)
                                                         gTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgctg                                               >  1:4421787/1‑38 (MQ=255)
                                                          tttATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATctgc                       >  1:1737934/1‑61 (MQ=255)
                                                          tttATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCgtg               <  1:3732176/69‑1 (MQ=255)
                                                            tATTCTGACGGCGGTGGATGCAGGTACGCGtgctgc                                              >  1:3637979/1‑36 (MQ=255)
                                                            tATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCgtggtg            >  1:3963062/1‑70 (MQ=255)
                                                            tATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGgtgt           >  1:5088644/1‑71 (MQ=255)
                                                             aTTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTAt                                     >  1:1715966/1‑44 (MQ=255)
                                                                cTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTcc       >  1:2346000/1‑71 (MQ=255)
                                                                   aCGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGgtgt           >  1:456495/1‑64 (MQ=255)
                                                                   aCGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGtc          <  1:4305653/65‑1 (MQ=255)
                                                                   aCGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTcc       <  1:262788/68‑1 (MQ=255)
                                                                   aCGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTCCTgg    >  1:3769119/1‑71 (MQ=255)
                                                                    cggcggTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCgt                <  1:3391405/58‑1 (MQ=255)
                                                                    cggcggTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTcc       <  1:269545/67‑1 (MQ=255)
                                                                     ggcggTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGtct         >  1:2776551/1‑64 (MQ=255)
                                                                     ggcggTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTCCTGGcc  >  1:1775749/1‑71 (MQ=255)
                                                                     |                                                                      
GGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTGGGCGTGGTGTCTCCTGGCC  >  Exported/386176‑386315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: