Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 387,112 T→C 7.8% L12L (TTA→CTA) ‡ ybdD → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported387,1120TC 7.8% 251.4 / 8.9 89L12L (TTA→CTA) ‡ybdDconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (49/33);  new base C (5/2);  total (54/35)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

CGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAA  >  Exported/387042‑387182
                                                                      |                                                                      
cGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATAtt                                                                        <  1:1985360/71‑1 (MQ=255)
cGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATAtt                                                                        <  1:1354769/71‑1 (MQ=255)
  gCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATAtt                                                                        >  1:5016663/1‑69 (MQ=255)
     ctctTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATAtt                                                                        <  1:3957458/66‑1 (MQ=255)
     atctTTAAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATAtt                                                                        <  1:3474884/65‑1 (MQ=255)
        tttCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAg                                                                     >  1:510136/1‑66 (MQ=255)
          tCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAgg                                                               <  1:4838640/70‑1 (MQ=255)
          tCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAgg                                                               <  1:436353/70‑1 (MQ=255)
           cAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAggcg                                                             >  1:5618781/1‑71 (MQ=255)
               aaCATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGgcggcg                                                          >  1:4153458/1‑70 (MQ=255)
                 cATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATAttt                                                                       >  1:5477619/1‑55 (MQ=255)
                   tGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCt                                                     <  1:2848884/71‑1 (MQ=255)
                        aGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgatg                                                 <  1:4917597/70‑1 (MQ=255)
                         gCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTgatgat                                               <  1:1957616/71‑1 (MQ=255)
                            gAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCa                                                                 >  1:215504/1‑50 (MQ=255)
                            gAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgatga                                                >  1:5376051/1‑67 (MQ=255)
                            gAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg                                            <  1:1631052/71‑1 (MQ=255)
                            gAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg                                            <  1:1790062/71‑1 (MQ=255)
                            gAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg                                            <  1:2177515/71‑1 (MQ=255)
                             aGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtg                                                    <  1:1440691/62‑1 (MQ=255)
                              ggCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTgatgat                                               >  1:4865496/1‑66 (MQ=255)
                               gCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAggcggc                                                           <  1:1628028/53‑1 (MQ=255)
                               gCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTg                                             <  1:1261203/67‑1 (MQ=255)
                                 cAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGc                                       >  1:3990063/1‑71 (MQ=255)
                                  aaGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCt                                                     <  1:4857845/56‑1 (MQ=255)
                                   aGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTc                                                                  <  1:675230/42‑1 (MQ=255)
                                   aGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTc                                                                  <  1:2949382/42‑1 (MQ=255)
                                   aGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAg                                                       >  1:3806213/1‑53 (MQ=255)
                                   aGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAg                                                       >  1:1387564/1‑53 (MQ=255)
                                   aGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg                                            <  1:172929/64‑1 (MQ=255)
                                    gATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAg                                                                     <  1:3068182/38‑1 (MQ=255)
                                     aTGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAggcgg                                                            >  1:5330562/1‑46 (MQ=255)
                                     aTGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                   >  1:4886846/1‑71 (MQ=255)
                                     aTGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                   >  1:1601391/1‑71 (MQ=255)
                                      tGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                  >  1:2949322/1‑71 (MQ=255)
                                        tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGa                                                         <  1:2905471/46‑1 (MQ=255)
                                        tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCt                                                     <  1:1866695/50‑1 (MQ=255)
                                        tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCt                                                     <  1:4828258/50‑1 (MQ=255)
                                        tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGt                                           >  1:1954260/1‑60 (MQ=255)
                                        tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGt                                           >  1:5027236/1‑60 (MQ=255)
                                        tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTa                                <  1:1122434/71‑1 (MQ=255)
                                         ttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                   >  1:2650542/1‑67 (MQ=255)
                                         ttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                               >  1:1661989/1‑71 (MQ=255)
                                         ttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                               >  1:2445598/1‑71 (MQ=255)
                                          tGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                               >  1:2450142/1‑70 (MQ=255)
                                          tGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                               >  1:2071149/1‑70 (MQ=255)
                                          tGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                               >  1:1729895/1‑70 (MQ=255)
                                              tCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTAt                                         <  1:5027329/56‑1 (MQ=255)
                                               cACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACaa                          >  1:2493443/1‑70 (MQ=255)
                                               cACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAAc                         >  1:4476256/1‑71 (MQ=255)
                                                aCTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCt                                                     >  1:2551315/1‑42 (MQ=255)
                                                aCTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAAc                         >  1:305563/1‑70 (MQ=255)
                                                 cTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAAc                         <  1:598127/69‑1 (MQ=255)
                                                   ggCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgatga                                                >  1:1795185/1‑44 (MQ=255)
                                                   ggCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTa                                          >  1:4115185/1‑50 (MQ=255)
                                                   ggCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTAt                                         >  1:596595/1‑51 (MQ=255)
                                                    gCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTg                                             <  1:27387/46‑1 (MQ=255)
                                                     cAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGt                    >  1:169441/1‑70 (MQ=255)
                                                      aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgatga                                                >  1:2336443/1‑41 (MQ=255)
                                                      aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg                                            <  1:981279/45‑1 (MQ=255)
                                                      aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg                                            <  1:2746943/45‑1 (MQ=255)
                                                      aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg                                            <  1:2263016/45‑1 (MQ=255)
                                                      aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                  >  1:5572510/1‑55 (MQ=255)
                                                      aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                  >  1:5565807/1‑55 (MQ=255)
                                                      aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTc                   >  1:4569649/1‑70 (MQ=255)
                                                        aaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtg                                                    <  1:3806854/35‑1 (MQ=255)
                                                         aGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGaa                >  1:3932333/1‑70 (MQ=255)
                                                         aGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc               <  1:2296528/71‑1 (MQ=255)
                                                           ccGGAAAATATTTAGGTCAGGCGGCGAAGCtgatga                                                >  1:4265816/1‑36 (MQ=255)
                                                           ccGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACa              >  1:4169278/1‑70 (MQ=255)
                                                            cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                   <  1:3742088/48‑1 (MQ=255)
                                                            cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                   <  1:4007465/48‑1 (MQ=255)
                                                            cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                   <  1:3234295/48‑1 (MQ=255)
                                                            cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACaa                          <  1:4582039/57‑1 (MQ=255)
                                                            aaaaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCata            >  1:1375512/4‑71 (MQ=255)
                                                            aaaaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCata            >  1:1368742/4‑71 (MQ=255)
                                                            aaaaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCata            >  1:2503899/4‑71 (MQ=255)
                                                              gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                        >  1:1563660/1‑57 (MQ=255)
                                                              gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                        >  1:3960817/1‑57 (MQ=255)
                                                              gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                        >  1:2710601/1‑57 (MQ=255)
                                                              gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                        >  1:4746026/1‑57 (MQ=255)
                                                              aaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg          <  1:2994722/70‑1 (MQ=255)
                                                              aaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg          <  1:784947/70‑1 (MQ=255)
                                                               aaaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                  >  1:1798256/1‑46 (MQ=255)
                                                               aaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTAc                               >  1:307246/1‑49 (MQ=255)
                                                               aaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg          >  1:2617577/1‑70 (MQ=255)
                                                                aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg        >  1:331928/1‑71 (MQ=255)
                                                                aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg        <  1:3826447/71‑1 (MQ=255)
                                                                aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg        >  1:405943/1‑71 (MQ=255)
                                                                  atatTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCggg      >  1:5142009/1‑71 (MQ=255)
                                                                    atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt     >  1:2606716/1‑70 (MQ=255)
                                                                    atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt     >  1:3267710/1‑70 (MQ=255)
                                                                    atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt     >  1:4408503/1‑70 (MQ=255)
                                                                    atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt     >  1:1579984/1‑70 (MQ=255)
                                                                    atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt     >  1:3907523/1‑70 (MQ=255)
                                                                     tttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa   <  1:3716080/71‑1 (MQ=255)
                                                                     tttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa   <  1:4862363/71‑1 (MQ=255)
                                                                      ttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTaa  >  1:2608251/1‑71 (MQ=255)
                                                                      ttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTaa  >  1:967722/1‑71 (MQ=255)
                                                                      |                                                                      
CGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAA  >  Exported/387042‑387182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: