Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 387,130 C→A 9.3% L18M (CTG→ATG)  ybdD → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported387,1300CA 9.3% 331.9 / 10.5 106L18M (CTG→ATG) ybdDconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (59/36);  new base A (7/3);  total (66/40)
Fisher's exact test for biased strand distribution p-value = 7.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-01

Only 100 of 118 total aligned reads displayed.

TGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCC  >  Exported/387061‑387200
                                                                     |                                                                      
tGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCt                                                                       <  1:2848884/71‑1 (MQ=255)
     aGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgatg                                                                   <  1:4917597/70‑1 (MQ=255)
      gCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTgatgat                                                                 <  1:1957616/71‑1 (MQ=255)
         gAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgatga                                                                  >  1:5376051/1‑67 (MQ=255)
         gAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg                                                              <  1:1790062/71‑1 (MQ=255)
         gAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg                                                              <  1:1631052/71‑1 (MQ=255)
           ggCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTgatgat                                                                 >  1:4865496/1‑66 (MQ=255)
            gCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTg                                                               <  1:1261203/67‑1 (MQ=255)
              cAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGc                                                         >  1:3990063/1‑71 (MQ=255)
               aaGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCt                                                                       <  1:4857845/56‑1 (MQ=255)
                aGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg                                                              <  1:172929/64‑1 (MQ=255)
                  aTGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                     >  1:1601391/1‑71 (MQ=255)
                   tGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                                    >  1:2949322/1‑71 (MQ=255)
                     tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCt                                                                       <  1:4828258/50‑1 (MQ=255)
                     tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCt                                                                       <  1:1866695/50‑1 (MQ=255)
                     tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGt                                                             >  1:1954260/1‑60 (MQ=255)
                     tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGt                                                             >  1:5027236/1‑60 (MQ=255)
                     tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTa                                                  <  1:1122434/71‑1 (MQ=255)
                      ttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                     >  1:2650542/1‑67 (MQ=255)
                      ttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                 >  1:1661989/1‑71 (MQ=255)
                       tGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                 >  1:2071149/1‑70 (MQ=255)
                       tGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                 >  1:2450142/1‑70 (MQ=255)
                       tGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                 >  1:1729895/1‑70 (MQ=255)
                           tCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTAt                                                           <  1:5027329/56‑1 (MQ=255)
                            cACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACaa                                            >  1:2493443/1‑70 (MQ=255)
                            cACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAAc                                           >  1:4476256/1‑71 (MQ=255)
                             aCTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCt                                                                       >  1:2551315/1‑42 (MQ=255)
                              cTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAAc                                           <  1:598127/69‑1 (MQ=255)
                                ggCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgatga                                                                  >  1:1795185/1‑44 (MQ=255)
                                ggCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTa                                                            >  1:4115185/1‑50 (MQ=255)
                                ggCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTAt                                                           >  1:596595/1‑51 (MQ=255)
                                   aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgatga                                                                  >  1:2336443/1‑41 (MQ=255)
                                   aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg                                                              <  1:2746943/45‑1 (MQ=255)
                                   aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg                                                              <  1:2263016/45‑1 (MQ=255)
                                   aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                                    >  1:5565807/1‑55 (MQ=255)
                                   aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTc                                     >  1:4569649/1‑70 (MQ=255)
                                     aaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtg                                                                      <  1:3806854/35‑1 (MQ=255)
                                      aGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                 <  1:2296528/71‑1 (MQ=255)
                                        ccGGAAAATATTTAGGTCAGGCGGCGAAGCtgatga                                                                  >  1:4265816/1‑36 (MQ=255)
                                         cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                     <  1:3742088/48‑1 (MQ=255)
                                         cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                     <  1:4007465/48‑1 (MQ=255)
                                         cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                     <  1:3234295/48‑1 (MQ=255)
                                         cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACaa                                            <  1:4582039/57‑1 (MQ=255)
                                         aaaaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCata                              >  1:1368742/4‑71 (MQ=255)
                                         aaaaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCata                              >  1:2503899/4‑71 (MQ=255)
                                         aaaaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCata                              >  1:1375512/4‑71 (MQ=255)
                                           gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                                          >  1:2710601/1‑57 (MQ=255)
                                           gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                                          >  1:3960817/1‑57 (MQ=255)
                                           gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                                          >  1:1563660/1‑57 (MQ=255)
                                           aaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                            <  1:2994722/70‑1 (MQ=255)
                                           aaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                            <  1:784947/70‑1 (MQ=255)
                                            aaaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                                    >  1:1798256/1‑46 (MQ=255)
                                            aaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTAc                                                 >  1:307246/1‑49 (MQ=255)
                                            aaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                            >  1:2617577/1‑70 (MQ=255)
                                             aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg                          >  1:405943/1‑71 (MQ=255)
                                             aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg                          <  1:3826447/71‑1 (MQ=255)
                                             aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg                          >  1:331928/1‑71 (MQ=255)
                                               atatTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCggg                        >  1:5142009/1‑71 (MQ=255)
                                                 atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                       >  1:1579984/1‑70 (MQ=255)
                                                 atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                       >  1:3907523/1‑70 (MQ=255)
                                                 atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                       >  1:2606716/1‑70 (MQ=255)
                                                 atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                       >  1:4408503/1‑70 (MQ=255)
                                                  tttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                     <  1:4862363/71‑1 (MQ=255)
                                                  tttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                     <  1:3716080/71‑1 (MQ=255)
                                                   ttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTaa                    >  1:967722/1‑71 (MQ=255)
                                                    tAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                   >  1:4330098/1‑71 (MQ=255)
                                                     aGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                   <  1:342600/70‑1 (MQ=255)
                                                           ggcggcGAAGCTGATGATTGGTATGCCTGATTACGACAACTa                                         >  1:1023549/1‑42 (MQ=255)
                                                              ggcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACata                              >  1:2670197/1‑50 (MQ=255)
                                                              ggcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACata                              >  1:4862206/1‑50 (MQ=255)
                                                              ggcgAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATgaa                          >  1:3133247/1‑52 (MQ=255)
                                                               gcgAAGTTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTaaac                  <  1:4942000/61‑3 (MQ=255)
                                                               gcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAcc                  <  1:4683993/61‑1 (MQ=255)
                                                               gcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCa        <  1:4922145/71‑1 (MQ=255)
                                                               gcgAAGATGTTGATTGGCATTCCAGACTACGACAACTacg                                       <  1:5546099/40‑3 (MQ=255)
                                                                cgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                     <  1:2599167/57‑1 (MQ=255)
                                                                 gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                 <  1:3675457/44‑1 (MQ=255)
                                                                 gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                 <  1:1450240/44‑1 (MQ=255)
                                                                 gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                 <  1:2855109/44‑1 (MQ=255)
                                                                 gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa      >  1:5554143/1‑71 (MQ=255)
                                                                  aaGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAAc     <  1:1930727/71‑1 (MQ=255)
                                                                   aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGaa                                  >  1:4688802/1‑41 (MQ=255)
                                                                   aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACat                               >  1:4705646/1‑44 (MQ=255)
                                                                   aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACat                               >  1:3906435/1‑44 (MQ=255)
                                                                    gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                         >  1:829640/1‑49 (MQ=255)
                                                                    gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                         >  1:141617/1‑49 (MQ=255)
                                                                    gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                         >  1:3400112/1‑49 (MQ=255)
                                                                    gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                         >  1:4724351/1‑49 (MQ=255)
                                                                    gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                         >  1:5383963/1‑49 (MQ=255)
                                                                     cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                   <  1:3402980/38‑1 (MQ=255)
                                                                     cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                   <  1:3094796/38‑1 (MQ=255)
                                                                     cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                   <  1:3186493/38‑1 (MQ=255)
                                                                     cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATc               >  1:3332601/1‑58 (MQ=255)
                                                                     cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATc         <  1:2425918/64‑1 (MQ=255)
                                                                     cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCa        >  1:2193423/1‑65 (MQ=255)
                                                                     cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc  <  1:2756149/71‑1 (MQ=255)
                                                                     cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc  <  1:4705111/71‑1 (MQ=255)
                                                                     cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc  <  1:263657/71‑1 (MQ=255)
                                                                     cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc  <  1:4857755/71‑1 (MQ=255)
                                                                     cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGc   >  1:1988167/1‑70 (MQ=255)
                                                                     |                                                                      
TGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCC  >  Exported/387061‑387200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: