Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 387,133 A→T 9.6% M19L (ATG→TTG)  ybdD → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported387,1330AT 9.6% 283.5 / 17.8 104M19L (ATG→TTG) ybdDconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (56/38);  new base T (7/3);  total (63/41)
Fisher's exact test for biased strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

AGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGAT  >  Exported/387066‑387203
                                                                   |                                                                      
aGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgatg                                                                      <  1:4917597/70‑1 (MQ=255)
 gCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTgatgat                                                                    <  1:1957616/71‑1 (MQ=255)
    gAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgatga                                                                     >  1:5376051/1‑67 (MQ=255)
    gAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg                                                                 <  1:2177515/71‑1 (MQ=255)
    gAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg                                                                 <  1:1790062/71‑1 (MQ=255)
      ggCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTgatgat                                                                    >  1:4865496/1‑66 (MQ=255)
       gCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTg                                                                  <  1:1261203/67‑1 (MQ=255)
         cAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGc                                                            >  1:3990063/1‑71 (MQ=255)
           aGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg                                                                 <  1:172929/64‑1 (MQ=255)
             aTGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                        >  1:4886846/1‑71 (MQ=255)
             aTGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                        >  1:1601391/1‑71 (MQ=255)
              tGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                                       >  1:2949322/1‑71 (MQ=255)
                tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGt                                                                >  1:5027236/1‑60 (MQ=255)
                tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGt                                                                >  1:1954260/1‑60 (MQ=255)
                tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTa                                                     <  1:1122434/71‑1 (MQ=255)
                 ttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                        >  1:2650542/1‑67 (MQ=255)
                 ttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                    >  1:1661989/1‑71 (MQ=255)
                 ttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                    >  1:2445598/1‑71 (MQ=255)
                  tGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                    >  1:2450142/1‑70 (MQ=255)
                      tCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTAt                                                              <  1:5027329/56‑1 (MQ=255)
                       cACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAAc                                              >  1:4476256/1‑71 (MQ=255)
                        aCTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAAc                                              >  1:305563/1‑70 (MQ=255)
                         cTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAAc                                              <  1:598127/69‑1 (MQ=255)
                           ggCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgatga                                                                     >  1:1795185/1‑44 (MQ=255)
                           ggCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTa                                                               >  1:4115185/1‑50 (MQ=255)
                           ggCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTAt                                                              >  1:596595/1‑51 (MQ=255)
                            gCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTg                                                                  <  1:27387/46‑1 (MQ=255)
                             cAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGt                                         >  1:169441/1‑70 (MQ=255)
                              aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgatga                                                                     >  1:2336443/1‑41 (MQ=255)
                              aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg                                                                 <  1:2746943/45‑1 (MQ=255)
                              aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                                       >  1:5572510/1‑55 (MQ=255)
                              aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                                       >  1:5565807/1‑55 (MQ=255)
                              aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTc                                        >  1:4569649/1‑70 (MQ=255)
                                 aGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGaa                                     >  1:3932333/1‑70 (MQ=255)
                                 aGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                    <  1:2296528/71‑1 (MQ=255)
                                   ccGGAAAATATTTAGGTCAGGCGGCGAAGCtgatga                                                                     >  1:4265816/1‑36 (MQ=255)
                                   ccGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACa                                   >  1:4169278/1‑70 (MQ=255)
                                    cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                        <  1:3234295/48‑1 (MQ=255)
                                    cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                        <  1:3742088/48‑1 (MQ=255)
                                    aaaaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCata                                 >  1:2503899/4‑71 (MQ=255)
                                    aaaaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCata                                 >  1:1375512/4‑71 (MQ=255)
                                    aaaaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCata                                 >  1:1368742/4‑71 (MQ=255)
                                      gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                                             >  1:4746026/1‑57 (MQ=255)
                                      gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                                             >  1:1563660/1‑57 (MQ=255)
                                      gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                                             >  1:3960817/1‑57 (MQ=255)
                                      aaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                               <  1:2994722/70‑1 (MQ=255)
                                       aaaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                                       >  1:1798256/1‑46 (MQ=255)
                                       aaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTAc                                                    >  1:307246/1‑49 (MQ=255)
                                        aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg                             >  1:331928/1‑71 (MQ=255)
                                        aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg                             >  1:405943/1‑71 (MQ=255)
                                        aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg                             <  1:3826447/71‑1 (MQ=255)
                                          atatTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCggg                           >  1:5142009/1‑71 (MQ=255)
                                            atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                          >  1:2606716/1‑70 (MQ=255)
                                            atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                          >  1:1579984/1‑70 (MQ=255)
                                            atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                          >  1:3907523/1‑70 (MQ=255)
                                            atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                          >  1:4408503/1‑70 (MQ=255)
                                             tttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                        <  1:3716080/71‑1 (MQ=255)
                                             tttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                        <  1:4862363/71‑1 (MQ=255)
                                              ttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTaa                       >  1:967722/1‑71 (MQ=255)
                                              ttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTaa                       >  1:2608251/1‑71 (MQ=255)
                                                aGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                      <  1:342600/70‑1 (MQ=255)
                                                      ggcggcGAAGCTGATGATTGGTATGCCTGATTACGACAACTa                                            >  1:1023549/1‑42 (MQ=255)
                                                         ggcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACata                                 >  1:2670197/1‑50 (MQ=255)
                                                         ggcgAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATgaa                             >  1:3133247/1‑52 (MQ=255)
                                                          gcgAAGTTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTaaac                     <  1:4942000/61‑3 (MQ=255)
                                                          gcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAcc                     <  1:4683993/61‑1 (MQ=255)
                                                          gcgAAGATGTTGATTGGCATTCCAGACTACGACAACTacg                                          <  1:5546099/40‑3 (MQ=255)
                                                           cgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                        <  1:2599167/57‑1 (MQ=255)
                                                            gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                    <  1:2855109/44‑1 (MQ=255)
                                                            gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                    <  1:1450240/44‑1 (MQ=255)
                                                              aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGaa                                     >  1:4688802/1‑41 (MQ=255)
                                                              aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACat                                  >  1:4705646/1‑44 (MQ=255)
                                                              aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACat                                  >  1:3678828/1‑44 (MQ=255)
                                                               gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                            >  1:3400112/1‑49 (MQ=255)
                                                               gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                            >  1:829640/1‑49 (MQ=255)
                                                               gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                            >  1:4724351/1‑49 (MQ=255)
                                                               gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                            >  1:5383963/1‑49 (MQ=255)
                                                               gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                            >  1:4655295/1‑49 (MQ=255)
                                                                cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                      <  1:3402980/38‑1 (MQ=255)
                                                                cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                      <  1:1829094/38‑1 (MQ=255)
                                                                cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                      <  1:3186493/38‑1 (MQ=255)
                                                                cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATc                  >  1:3332601/1‑58 (MQ=255)
                                                                cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATc            <  1:2425918/64‑1 (MQ=255)
                                                                cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCa           >  1:2193423/1‑65 (MQ=255)
                                                                cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc     <  1:263657/71‑1 (MQ=255)
                                                                cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc     <  1:4857755/71‑1 (MQ=255)
                                                                cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc     <  1:2756149/71‑1 (MQ=255)
                                                                cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGc      >  1:1988167/1‑70 (MQ=255)
                                                                 tgatgaTTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa         >  1:4794735/1‑66 (MQ=255)
                                                                 tgatgaTTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa         >  1:4691313/1‑66 (MQ=255)
                                                                 tgatgaTTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCa           <  1:2149469/64‑1 (MQ=255)
                                                                  gatgatTGGTATGCCTGATTACGACAACTATGTCGAAc                                    >  1:504197/1‑38 (MQ=255)
                                                                  gatgatTGGTATGCCTGATTACGACAACTATGTCGAAc                                    >  1:1959478/1‑38 (MQ=255)
                                                                  gatgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                        >  1:938213/1‑50 (MQ=255)
                                                                  gatgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGa   >  1:4499753/1‑71 (MQ=255)
                                                                   atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                      >  1:2251007/1‑51 (MQ=255)
                                                                   atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                      >  1:1637707/1‑51 (MQ=255)
                                                                   atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa         >  1:3227089/1‑64 (MQ=255)
                                                                   atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa         >  1:790725/1‑64 (MQ=255)
                                                                   atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGAt  <  1:2675114/71‑1 (MQ=255)
                                                                   |                                                                      
AGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGAT  >  Exported/387066‑387203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: