Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 387,141 T→C 11.7% G21G (GGT→GGC ybdD → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported387,1410TC11.7% 305.4 / 24.3 119G21G (GGT→GGCybdDconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (71/34);  new base C (8/6);  total (79/40)
Fisher's exact test for biased strand distribution p-value = 5.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

CAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACG  >  Exported/387075‑387211
                                                                  |                                                                      
cAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGc                                                                    >  1:3990063/1‑71 (MQ=255)
    aTGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                                >  1:4886846/1‑71 (MQ=255)
    aTGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                                >  1:1601391/1‑71 (MQ=255)
     tGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                                               >  1:2949322/1‑71 (MQ=255)
       tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGt                                                                        >  1:5027236/1‑60 (MQ=255)
       tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGt                                                                        >  1:1954260/1‑60 (MQ=255)
       tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTa                                                             <  1:1122434/71‑1 (MQ=255)
        ttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                                >  1:2650542/1‑67 (MQ=255)
        ttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                            >  1:1661989/1‑71 (MQ=255)
        ttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                            >  1:2445598/1‑71 (MQ=255)
         tGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                            >  1:2450142/1‑70 (MQ=255)
         tGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                            >  1:2071149/1‑70 (MQ=255)
             tCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTAt                                                                      <  1:5027329/56‑1 (MQ=255)
              cACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACaa                                                       >  1:2493443/1‑70 (MQ=255)
              cACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAAc                                                      >  1:4476256/1‑71 (MQ=255)
               aCTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAAc                                                      >  1:305563/1‑70 (MQ=255)
                cTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAAc                                                      <  1:598127/69‑1 (MQ=255)
                  ggCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTAt                                                                      >  1:596595/1‑51 (MQ=255)
                    cAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGt                                                 >  1:169441/1‑70 (MQ=255)
                     aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                                               >  1:5572510/1‑55 (MQ=255)
                     aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                                               >  1:5565807/1‑55 (MQ=255)
                     aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTc                                                >  1:4569649/1‑70 (MQ=255)
                        aGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGaa                                             >  1:3932333/1‑70 (MQ=255)
                        aGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                            <  1:2296528/71‑1 (MQ=255)
                          ccGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACa                                           >  1:4169278/1‑70 (MQ=255)
                           cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                                <  1:4007465/48‑1 (MQ=255)
                           cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                                <  1:3234295/48‑1 (MQ=255)
                           cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                                                <  1:3742088/48‑1 (MQ=255)
                           cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACaa                                                       <  1:4582039/57‑1 (MQ=255)
                           aaaaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCata                                         >  1:1368742/4‑71 (MQ=255)
                             gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                                                     >  1:3960817/1‑57 (MQ=255)
                             gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                                                     >  1:2710601/1‑57 (MQ=255)
                             gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                                                     >  1:1563660/1‑57 (MQ=255)
                              aaaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                                               >  1:1798256/1‑46 (MQ=255)
                              aaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTAc                                                            >  1:307246/1‑49 (MQ=255)
                               aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg                                     >  1:405943/1‑71 (MQ=255)
                               aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg                                     <  1:3826447/71‑1 (MQ=255)
                               aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg                                     >  1:331928/1‑71 (MQ=255)
                                 atatTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCggg                                   >  1:5142009/1‑71 (MQ=255)
                                   atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                  >  1:3267710/1‑70 (MQ=255)
                                   atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                  >  1:3907523/1‑70 (MQ=255)
                                   atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                  >  1:2606716/1‑70 (MQ=255)
                                   atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                  >  1:4408503/1‑70 (MQ=255)
                                     ttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTaa                               >  1:2608251/1‑71 (MQ=255)
                                      tAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                              >  1:4330098/1‑71 (MQ=255)
                                       aGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                              <  1:342600/70‑1 (MQ=255)
                                             ggcggcGAAGCTGATGATTGGTATGCCTGATTACGACAACTa                                                    >  1:1023549/1‑42 (MQ=255)
                                               cggcgAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATgaa                                     >  1:5111859/1‑53 (MQ=255)
                                                ggcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACata                                         >  1:4862206/1‑50 (MQ=255)
                                                ggcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACata                                         >  1:2670197/1‑50 (MQ=255)
                                                ggcgAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATgaa                                     >  1:3133247/1‑52 (MQ=255)
                                                 gcgAAGTTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTaaac                             <  1:4942000/61‑3 (MQ=255)
                                                 gcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAcc                             <  1:4683993/61‑1 (MQ=255)
                                                 gcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCa                   <  1:4922145/71‑1 (MQ=255)
                                                  cgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                                <  1:2599167/57‑1 (MQ=255)
                                                   gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                            <  1:3675457/44‑1 (MQ=255)
                                                   gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                            <  1:1450240/44‑1 (MQ=255)
                                                   gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                            <  1:2855109/44‑1 (MQ=255)
                                                   gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:5554143/1‑71 (MQ=255)
                                                    aaGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAAc                <  1:1930727/71‑1 (MQ=255)
                                                     aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACat                                          >  1:3678828/1‑44 (MQ=255)
                                                     aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACat                                          >  1:3906435/1‑44 (MQ=255)
                                                     aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACat                                          >  1:4705646/1‑44 (MQ=255)
                                                      gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                    >  1:141617/1‑49 (MQ=255)
                                                      gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                    >  1:829640/1‑49 (MQ=255)
                                                      gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                    >  1:4655295/1‑49 (MQ=255)
                                                      gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                    >  1:3400112/1‑49 (MQ=255)
                                                      gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                    >  1:4724351/1‑49 (MQ=255)
                                                      gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                    >  1:5383963/1‑49 (MQ=255)
                                                       cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                              <  1:3094796/38‑1 (MQ=255)
                                                       cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                              <  1:1829094/38‑1 (MQ=255)
                                                       cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                              <  1:3402980/38‑1 (MQ=255)
                                                       cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATc                    <  1:2425918/64‑1 (MQ=255)
                                                       cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCa                   >  1:2193423/1‑65 (MQ=255)
                                                       cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc             <  1:4705111/71‑1 (MQ=255)
                                                       cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc             <  1:263657/71‑1 (MQ=255)
                                                       cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc             <  1:4857755/71‑1 (MQ=255)
                                                        tgatgaTTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:4691313/1‑66 (MQ=255)
                                                        tgatgaTTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:4794735/1‑66 (MQ=255)
                                                        tgatgaTTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCa                   <  1:2149469/64‑1 (MQ=255)
                                                         gatgatTGGTATGCCTGATTACGACAACTATGTCGAAc                                            >  1:1959478/1‑38 (MQ=255)
                                                         gatgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                                >  1:938213/1‑50 (MQ=255)
                                                         gatgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCg            <  1:2939755/70‑1 (MQ=255)
                                                          ttgatTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                       <  1:4783303/41‑1 (MQ=255)
                                                          ttgatTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                       <  1:2812804/41‑1 (MQ=255)
                                                          ttgatTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                       <  1:2616689/41‑1 (MQ=255)
                                                          atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                              >  1:1637707/1‑51 (MQ=255)
                                                          atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:3227089/1‑64 (MQ=255)
                                                          atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:790725/1‑64 (MQ=255)
                                                          atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGAt          <  1:2675114/71‑1 (MQ=255)
                                                           tgatTGGTATGCCTGATTACGACAACTATGTCGAACata                                         >  1:5366212/1‑39 (MQ=255)
                                                           tgatTGGTATGCCTGATTACGACAACTATGTCGAACata                                         >  1:1283650/1‑39 (MQ=255)
                                                           tgatTGGCATTCCAGACTACGACAACTACGTCGAGCATATgaa                                     >  1:1488582/1‑41 (MQ=255)
                                                            gatTGGTATGCCTGATTACGACAACTATGTCGAACa                                           >  1:3130891/1‑36 (MQ=255)
                                                            gatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:1087035/1‑62 (MQ=255)
                                                            gatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:906862/1‑62 (MQ=255)
                                                              ttGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGAcc      >  1:1485617/1‑71 (MQ=255)
                                                               tGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                 >  1:4126632/1‑59 (MQ=255)
                                                                 gTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGAcc      <  1:4742001/68‑1 (MQ=255)
                                                                  tATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACg  <  1:2161725/71‑1 (MQ=255)
                                                                  |                                                                      
CAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACG  >  Exported/387075‑387211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: