| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 189,194 | G→T | 17.3% | A231S (GCC→TCC) ‡ | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 189,194 | 0 | G | T | 17.3% | 154.7 / 15.3 | 59 | A231S (GCC→TCC) ‡ | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (26/22); new base T (5/5); total (32/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGAT > Exported/189126‑189261 | ttGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTAcgcc > 1:2600101/1‑71 (MQ=255)ttGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTAcgc < 1:3486785/70‑1 (MQ=255) aCAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCAGCGCCATcc > 1:4556846/1‑70 (MQ=255) agTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAg < 1:975876/71‑1 (MQ=255) agTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACCCCGCGCCATCCGGCAg > 1:3363731/1‑71 (MQ=255) ttATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAg < 1:1045797/69‑1 (MQ=255) ttATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAg < 1:1249830/69‑1 (MQ=255) ttATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAg < 1:3058383/69‑1 (MQ=255) ttATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAg < 1:4825667/69‑1 (MQ=255) ttATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAg < 1:510882/69‑1 (MQ=255) atatCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACg > 1:935480/1‑71 (MQ=255) tCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGc > 1:4258031/1‑69 (MQ=255) tCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTg < 1:5031045/71‑1 (MQ=255) cGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATc > 1:1838877/1‑57 (MQ=255) cGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCg < 1:4374689/59‑1 (MQ=255) cGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCg < 1:4310534/59‑1 (MQ=255) cGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGt > 1:1360436/1‑71 (MQ=255) gTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGtt > 1:5463265/1‑71 (MQ=255) gTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGtt < 1:5474917/71‑1 (MQ=255) gTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGtt > 1:3171354/1‑71 (MQ=255) cAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCg < 1:2149679/71‑1 (MQ=255) cAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCg < 1:1698571/71‑1 (MQ=255) aaCGGAACATCGACGTCGAACAAAATTGTGGGTATGTAcgcc > 1:2951983/1‑42 (MQ=255) aaCGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGa > 1:4479159/1‑71 (MQ=255) aaCATCGACGTCGAACAAAATTGTGGGTATTTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGc > 1:4730973/1‑70 (MQ=255) aCATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCaa < 1:3115149/71‑1 (MQ=255) aCATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCaa < 1:2695939/71‑1 (MQ=255) tCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTg < 1:494279/54‑1 (MQ=255) tCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTg < 1:4299323/54‑1 (MQ=255) gACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCa > 1:1845794/1‑44 (MQ=255) gACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCa > 1:4508232/1‑44 (MQ=255) gACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCa > 1:4645180/1‑44 (MQ=255) gACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTa > 1:905759/1‑47 (MQ=255) gACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTa > 1:4824033/1‑47 (MQ=255) gACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCt > 1:5535877/1‑51 (MQ=255) aCGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGt < 1:3666901/69‑1 (MQ=255) cGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCa > 1:1151564/1‑70 (MQ=255) gcGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCaca > 1:1889760/2‑68 (MQ=255) gcGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCaca > 1:5164249/2‑68 (MQ=255) gcGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCaca > 1:352896/2‑68 (MQ=255) cGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCaca < 1:4449368/69‑3 (MQ=255) cGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCaca < 1:5103456/69‑3 (MQ=255) cGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCACaaa < 1:3650730/71‑1 (MQ=255) cGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCACaaa < 1:5229770/71‑1 (MQ=255) cGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCACaaa > 1:5292984/1‑71 (MQ=255) cGAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCACaa < 1:4096418/70‑1 (MQ=255) gAACAAAATTGTTGGTATGTACTCTGCTCCAGCAGGCAGCACCATTCTGATTGACCGTAACTGCCACAAAt > 1:1707822/1‑71 (MQ=255) aaCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATc > 1:2954425/1‑71 (MQ=255) aaCAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATc > 1:2947894/1‑71 (MQ=255) aaaaTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCt > 1:1921515/1‑71 (MQ=255) aaaTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGAc > 1:410526/1‑50 (MQ=255) aaaTTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGAc > 1:5329326/1‑50 (MQ=255) ttGTGGGTATGTACGCCGCGCCATCCGGCAGTACTCTGTTGATc < 1:4624307/44‑1 (MQ=255) ttGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATc < 1:2222198/44‑1 (MQ=255) ggTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATc < 1:3370142/70‑1 (MQ=255) tGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGtt < 1:5654539/70‑1 (MQ=255) tGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGtt > 1:4453286/1‑70 (MQ=255) tACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTtgat < 1:1870605/71‑1 (MQ=255) tACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTtgat > 1:1613896/1‑71 (MQ=255) aCGCCGCGCCATCCGGCAGTACGCTGTTGATCGCCCGCAATTGTCATAAATCGCTGGCGCATCTGTtgat > 1:2760333/1‑70 (MQ=255) aCGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTtgat > 1:3583222/1‑70 (MQ=255) aCGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTtgat > 1:1351555/1‑70 (MQ=255) | TTGGCGCGGAACAGAGTTATATCGTTACCAACGGAACATCGACGTCGAACAAAATTGTGGGTATGTACGCCGCGCCATCCGGCAGTACGCTGTTGATCGACCGCAATTGTCATAAATCGCTGGCGCATCTGTTGAT > Exported/189126‑189261 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |