Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 453,891 G→C 6.9% A398A (GCC→GCG ybgH ← predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported453,8910GC 6.9% 212.8 / 5.2 73A398A (GCC→GCGybgHpredicted transporter
Reads supporting (aligned to +/- strand):  ref base G (38/29);  new base C (3/2);  total (41/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

AAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAA  >  Exported/453822‑453960
                                                                     |                                                                     
aaGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATgg                                                                      >  1:1560343/1‑71 (MQ=255)
aaGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATgg                                                                      <  1:1867766/71‑1 (MQ=255)
        tGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCggg                                                              >  1:3191217/1‑71 (MQ=255)
        tGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCggg                                                              <  1:457871/71‑1 (MQ=255)
        tGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCggg                                                              <  1:1996475/71‑1 (MQ=255)
        tGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCggg                                                              <  1:3208593/71‑1 (MQ=255)
          tAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTc                                                            >  1:1880966/1‑71 (MQ=255)
          tAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTc                                                            >  1:265231/1‑71 (MQ=255)
              tGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGAt                                                         >  1:4846603/1‑70 (MQ=255)
              tGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATa                                                        <  1:1441290/71‑1 (MQ=255)
              tGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATa                                                        <  1:2435023/71‑1 (MQ=255)
               gCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATa                                                        <  1:2931615/70‑1 (MQ=255)
                ccGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATaaa                                                      <  1:4444835/71‑1 (MQ=255)
                   gTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAg                                                   >  1:4184714/1‑71 (MQ=255)
                      aaTACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGtt                                                 <  1:810175/70‑1 (MQ=255)
                      aaTACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGtt                                                 <  1:1686551/70‑1 (MQ=255)
                          cGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGc                                             >  1:842821/1‑70 (MQ=255)
                            ccGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCaaa                                          <  1:5291374/71‑1 (MQ=255)
                                 cacaCCGGGGATTTCAATGCGCGTAATTTGCGACATGGCa                                                                    <  1:3417779/40‑1 (MQ=255)
                                 cacaCCGGGGATTTCAATGCGCGTAATTTGCGACATGGCa                                                                    <  1:833701/40‑1 (MQ=255)
                                 cacaCCGGGGATTTCAATGCGCGTAATTTGCGACATGGCa                                                                    <  1:3103625/40‑1 (MQ=255)
                                 cacaCCGGGGATTTCAATGCGCGTAATTAGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGccc                                      >  1:2239834/1‑70 (MQ=255)
                                  acacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCAt                                    >  1:4220582/1‑71 (MQ=255)
                                   cacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAAc                                                     <  1:5337443/53‑1 (MQ=255)
                                   cacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAAc                                                     <  1:1644636/53‑1 (MQ=255)
                                   cacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAAc                                                     <  1:158017/53‑1 (MQ=255)
                                   cacCGGAGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAACGCCCAt                                    >  1:5900/1‑70 (MQ=255)
                                    acCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAAc                                                                  >  1:3193616/1‑39 (MQ=255)
                                    acCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAAc                                                                  >  1:3661368/1‑39 (MQ=255)
                                     ccGGGGATTTCAATGCGCGTAATTTGCGACATGGCAAc                                                                  >  1:3610001/1‑38 (MQ=255)
                                     ccGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATa                                                        >  1:2512915/1‑48 (MQ=255)
                                      cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCAcc                                >  1:2404714/1‑71 (MQ=255)
                                      cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCAcc                                >  1:2310920/1‑71 (MQ=255)
                                      cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCAcc                                >  1:4167049/1‑71 (MQ=255)
                                      cGGGGATTTCAATGCGCCTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCaaa                                          <  1:345052/61‑1 (MQ=255)
                                       ggggATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCg                               >  1:11578/1‑71 (MQ=255)
                                        gggATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATaaa                                                      >  1:3139831/1‑47 (MQ=255)
                                        gggATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATaaa                                                      >  1:5340545/1‑47 (MQ=255)
                                          gATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAg                                         >  1:4070724/1‑58 (MQ=255)
                                           aTTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGcc                             <  1:957240/69‑1 (MQ=255)
                                           aTTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAg                           >  1:4179011/1‑71 (MQ=255)
                                            tttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAgg                          >  1:2737780/1‑71 (MQ=255)
                                            tttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAgg                          >  1:4826648/1‑71 (MQ=255)
                                             ttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCAt                                    <  1:3018891/60‑1 (MQ=255)
                                               cAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATaaa                                                      >  1:1095301/1‑40 (MQ=255)
                                                  tGCGCGTAATTTGCGACATTGCAACCGGGTCGATa                                                        <  1:5176365/35‑1 (MQ=255)
                                                  tGCGCGTAATTTGCGACATGGCAACCGGGTCGATa                                                        <  1:3526013/35‑1 (MQ=255)
                                                  tGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                     >  1:196897/1‑70 (MQ=255)
                                                  tGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAAt                    <  1:492960/71‑1 (MQ=255)
                                                   gcgcGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCa                            >  1:3699689/1‑62 (MQ=255)
                                                    cgcgTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGccc                                      >  1:3348645/1‑51 (MQ=255)
                                                       gtgaTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCATTAGCGCCAGCCCGGATATCAt               >  1:4427525/4‑71 (MQ=255)
                                                        tAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCt                       <  1:3640485/62‑1 (MQ=255)
                                                        tAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCt                       <  1:3508119/62‑1 (MQ=255)
                                                         aaTTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCaaa                                          >  1:3765145/1‑42 (MQ=255)
                                                          aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCa                                     >  1:5094101/1‑46 (MQ=255)
                                                          aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCa                                     >  1:3732216/1‑46 (MQ=255)
                                                          aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCATTAGCGCCAGCCCGGATAtcatcac            <  1:4273078/71‑2 (MQ=255)
                                                          aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCATTAGCGCCAGCCCGGATAtcatcac            <  1:2031314/71‑2 (MQ=255)
                                                          aTTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAAt                    >  1:2499133/1‑63 (MQ=255)
                                                           tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGc                                             <  1:4969394/37‑1 (MQ=255)
                                                           tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGc                                             <  1:3443255/37‑1 (MQ=255)
                                                           tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                     >  1:4457961/1‑61 (MQ=255)
                                                           tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                     >  1:937381/1‑61 (MQ=255)
                                                           tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                     >  1:315219/1‑61 (MQ=255)
                                                           tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                     >  1:2579874/1‑61 (MQ=255)
                                                           tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                     >  1:655023/1‑61 (MQ=255)
                                                             tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCa                                  >  1:93137/1‑46 (MQ=255)
                                                             tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGTCCTAATACCATCAGTgg         >  1:199680/1‑71 (MQ=255)
                                                             tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTgg         >  1:542266/1‑71 (MQ=255)
                                                                gACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCag      <  1:63939/71‑1 (MQ=255)
                                                                 aCATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCa       >  1:2817230/1‑69 (MQ=255)
                                                                    tGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa  <  1:1431740/71‑1 (MQ=255)
                                                                    tGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa  <  1:4152717/71‑1 (MQ=255)
                                                                    tGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa  <  1:496719/71‑1 (MQ=255)
                                                                     |                                                                     
AAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAA  >  Exported/453822‑453960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: