BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	454,715	G→A	14.5%	L124L (CTG→TTG)	ybgH ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	454,715	0	G	A	14.5%	251.4 / 27.6	103	L124L (CTG→TTG)	ybgH	predicted transporter
Reads supporting (aligned to +/- strand): ref base G (46/42); new base A (8/7); total (54/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 103 total aligned reads displayed.

CGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGA  >  Exported/454650‑454784

agcATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAAc                                                                     <  1:5091729/67‑1 (MQ=255)

agcATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAg                                                                   >  1:4744271/2‑70 (MQ=255)

  cATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCt                                                                 >  1:4100189/1‑70 (MQ=255)

   aTACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtga                                                               >  1:49635/1‑69 (MQ=255)

    tACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtgat                                                              >  1:296372/1‑68 (MQ=255)

    tACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtgat                                                              >  1:3753874/1‑68 (MQ=255)

      cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                                            >  1:1176694/1‑71 (MQ=255)

      cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                                            >  1:4004165/1‑71 (MQ=255)

       aTCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGtt                                                           <  1:3866004/71‑1 (MQ=255)

         cAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGttt                                                          >  1:4326694/1‑69 (MQ=255)

          aGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGa                                                        <  1:1171842/71‑1 (MQ=255)

             gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                                                      >  1:4574586/1‑70 (MQ=255)

             gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                                                      >  1:2078218/1‑70 (MQ=255)

             gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                                                      >  1:4849648/1‑70 (MQ=255)

             gaaaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAttt                                                     <  1:1086908/68‑1 (MQ=255)

              agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                    <  1:1105808/71‑1 (MQ=255)

              agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                    <  1:3817507/71‑1 (MQ=255)

              agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                    <  1:742179/71‑1 (MQ=255)

              agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                    <  1:2163150/71‑1 (MQ=255)

              agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                    <  1:1109709/71‑1 (MQ=255)

              aaaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttg                                                    <  1:1687386/69‑2 (MQ=255)

               aaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttga                                                   <  1:1109761/70‑3 (MQ=255)

               aaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttga                                                   <  1:3393136/70‑3 (MQ=255)

               aaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttg                                                    >  1:674426/2‑69 (MQ=255)

                aaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtga                                                               >  1:1477802/1‑56 (MQ=255)

                aaaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                    >  1:2577328/1‑69 (MQ=255)

                 aaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                                  <  1:1567786/70‑1 (MQ=255)

                 aaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAAc                                                 <  1:5627258/71‑1 (MQ=255)

                 aaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAAc                                                 <  1:5510105/71‑1 (MQ=255)

                  aCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                                  >  1:3771252/1‑69 (MQ=255)

                  aCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                                  >  1:2965054/1‑69 (MQ=255)

                   ccGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGATTTgaataa                                               <  1:3078987/71‑4 (MQ=255)

                     gCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAttt                                                     >  1:1372152/1‑63 (MQ=255)

                      ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                                                              >  1:1110962/1‑53 (MQ=255)

                      ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                                                              >  1:5221943/1‑53 (MQ=255)

                      ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                                                              >  1:5176542/1‑53 (MQ=255)

                      ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                                                      <  1:3610407/61‑1 (MQ=255)

                       cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                                  >  1:696834/1‑64 (MQ=255)

                       cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                                  >  1:2182490/1‑64 (MQ=255)

                         tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGAc                                                                     <  1:4230463/43‑1 (MQ=255)

                         tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGtt                                                           <  1:4060548/53‑1 (MQ=255)

                         tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGtt                                                           <  1:3970061/53‑1 (MQ=255)

                         tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                    <  1:3263689/60‑1 (MQ=255)

                          cACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGAc                                                                     <  1:3938897/42‑1 (MQ=255)

                           acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                                                      >  1:2797306/1‑40 (MQ=255)

                           acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg                                                                   >  1:2279261/1‑43 (MQ=255)

                           acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                                 <  1:3996830/45‑1 (MQ=255)

                           acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                                 <  1:321950/45‑1 (MQ=255)

                           acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                                 <  1:2100564/45‑1 (MQ=255)

                           acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                                 <  1:3711023/45‑1 (MQ=255)

                           acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGc                                       >  1:3275336/1‑71 (MQ=255)

                             gacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg                                     >  1:3473341/1‑71 (MQ=255)

                             gacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg                                     <  1:1205343/71‑1 (MQ=255)

                              acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                                            <  1:2854758/47‑1 (MQ=255)

                              acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                                            <  1:3498253/47‑1 (MQ=255)

                              acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaa                                                   <  1:2667699/56‑1 (MQ=255)

                                   gATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                                  >  1:2236478/1‑52 (MQ=255)

                                   gATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                    <  1:5402684/50‑1 (MQ=255)

                                   gATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACg                               <  1:1842496/71‑1 (MQ=255)

                                    aTCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAg                                                         <  1:163790/44‑1 (MQ=255)

                                      cGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                                                       >  1:4519623/1‑44 (MQ=255)

                                      cGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                                                       >  1:692470/1‑44 (MQ=255)

                                       ggTTGGCTCATACAGCTCGCCGAGCAGACAGCTTa                                                               >  1:3884009/1‑35 (MQ=255)

                                       ggTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATa                            <  1:3777455/70‑1 (MQ=255)

                                           ggCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc                       >  1:3050148/1‑71 (MQ=255)

                                           ggCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc                       >  1:5072854/1‑71 (MQ=255)

                                            gCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc                       <  1:4311849/70‑1 (MQ=255)

                                              tCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGAc                                >  1:5183545/1‑59 (MQ=255)

                                               cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg                   >  1:584049/1‑71 (MQ=255)

                                               cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg                   >  1:5188323/1‑71 (MQ=255)

                                                aTACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                                  >  1:4143060/1‑39 (MQ=255)

                                                aTACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaa                                                   <  1:5125345/38‑1 (MQ=255)

                                                aTACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATa                            <  1:418905/61‑1 (MQ=255)

                                                 tACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                    <  1:2009076/36‑1 (MQ=255)

                                                 tACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                    <  1:2368459/36‑1 (MQ=255)

                                                 tACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                    <  1:5052421/36‑1 (MQ=255)

                                                 tACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg                                     <  1:4566114/51‑1 (MQ=255)

                                                 tACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGa                 <  1:947705/71‑1 (MQ=255)

                                                  aCAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATa                            >  1:3571251/1‑59 (MQ=255)

                                                  aCAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGa                 >  1:635432/1‑70 (MQ=255)

                                                   cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAAc                                                 >  1:3553263/1‑37 (MQ=255)

                                                   cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCAt                                          >  1:5319322/1‑44 (MQ=255)

                                                   cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCAt                                          >  1:1600839/1‑44 (MQ=255)

                                                   cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCAt                                          >  1:5172477/1‑44 (MQ=255)

                                                   cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCAt                                          >  1:3776662/1‑44 (MQ=255)

                                                   cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATc                         >  1:2676194/1‑61 (MQ=255)

                                                   cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAg                    >  1:3311335/1‑66 (MQ=255)

                                                   cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAg                    >  1:647220/1‑66 (MQ=255)

                                                   cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGa                 >  1:5314031/1‑69 (MQ=255)

                                                   cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGa                 >  1:1643201/1‑69 (MQ=255)

                                                     gCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGa             <  1:1723237/71‑1 (MQ=255)

                                                     gCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGa             <  1:908907/71‑1 (MQ=255)

                                                         gCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAAt                          >  1:493776/1‑54 (MQ=255)

                                                         gCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATag          <  1:2707101/70‑1 (MQ=255)

                                                         gCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATa           <  1:4056450/69‑1 (MQ=255)

                                                         gCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATa           >  1:4708379/1‑69 (MQ=255)

                                                         gCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATa           >  1:2761581/1‑69 (MQ=255)

                                                            gAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAgg       >  1:1565390/1‑70 (MQ=255)

                                                              gCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAAt    <  1:1799607/71‑1 (MQ=255)

                                                                aGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGa  >  1:358902/1‑71 (MQ=255)

CGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGA  >  Exported/454650‑454784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`