BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	454,994	G→A	33.3%	L31L (CTG→TTG) ‡	ybgH ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	454,994	0	G	A	33.3%	111.3 / 79.2	96	L31L (CTG→TTG) ‡	ybgH	predicted transporter
Reads supporting (aligned to +/- strand): ref base G (23/41); new base A (13/19); total (36/60)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

Only 100 of 107 total aligned reads displayed.

AATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGG  >  Exported/454933‑455063

aaTAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATcagcag                                                                       >  1:579540/1‑62 (MQ=255)

aaTAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCAt                                                              >  1:4850534/1‑71 (MQ=255)

  tAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCGGCAGGGCACGCATGc                                                            >  1:5521668/1‑71 (MQ=255)

           ggCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGc                                                   >  1:1243234/1‑71 (MQ=255)

              gTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAATAGCTaa                                                <  1:2381/71‑1 (MQ=255)

              gTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTaa                                                <  1:3644714/71‑1 (MQ=255)

              gTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTaa                                                <  1:4791161/71‑1 (MQ=255)

               tGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTaaa                                               <  1:1362548/71‑1 (MQ=255)

                 aGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAat                                             <  1:3626119/71‑1 (MQ=255)

                   tATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAatat                                           <  1:3961095/71‑1 (MQ=255)

                    aTCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTaa                                                <  1:3515809/65‑1 (MQ=255)

                     tCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTaa                                                <  1:46847/64‑1 (MQ=255)

                      cGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTaa                                                <  1:4189223/63‑1 (MQ=255)

                       gTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTcc                                        >  1:2946076/1‑70 (MQ=255)

                       gTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTcc                                        >  1:342150/1‑70 (MQ=255)

                       gTTGTATTTTAGATGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATAtt                                          >  1:5247165/1‑68 (MQ=255)

                         tGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTcc                                        >  1:776356/1‑68 (MQ=255)

                              tttAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTcc                                        <  1:1029401/63‑1 (MQ=255)

                               ttAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTg                                <  1:414974/70‑1 (MQ=255)

                                  gTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAg                             <  1:5079031/70‑1 (MQ=255)

                                   ttGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTaa                                                <  1:2818561/50‑1 (MQ=255)

                                   ttGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTa                                                 <  1:5027261/49‑1 (MQ=255)

                                   ttGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAgcg                           >  1:2372739/1‑71 (MQ=255)

                                   ttGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAg                             <  1:1774744/69‑1 (MQ=255)

                                   ttGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAg                             <  1:2972474/69‑1 (MQ=255)

                                    tGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAgcg                           >  1:1587338/1‑70 (MQ=255)

                                     gATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTaaaa                                              >  1:1941796/1‑50 (MQ=255)

                                     gATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTaaaa                                              >  1:5095262/1‑50 (MQ=255)

                                     gATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTaaaa                                              >  1:2646619/1‑50 (MQ=255)

                                     gATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAatat                                           <  1:782649/53‑1 (MQ=255)

                                     gATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAgcgc                          >  1:596092/1‑70 (MQ=255)

                                     gATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAgcgc                          >  1:1619090/1‑70 (MQ=255)

                                       ttGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTaaaa                                              <  1:4013472/48‑1 (MQ=255)

                                       ttGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTaaaa                                              <  1:686172/48‑1 (MQ=255)

                                       ttGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTaaaa                                              <  1:1316045/48‑1 (MQ=255)

                                       ttGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAAc                       >  1:2081188/1‑71 (MQ=255)

                                       tgggTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCAc                       <  1:3353582/69‑1 (MQ=255)

                                       tgggTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCAc                       <  1:3862299/69‑1 (MQ=255)

                                       tgggTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCAc                       <  1:3263095/69‑1 (MQ=255)

                                        gggTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACtg                                                 <  1:1240955/43‑2 (MQ=25)

                                        gggTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTc                                         >  1:5201728/2‑52 (MQ=255)

                                          gTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGAt                                   >  1:4363715/1‑56 (MQ=255)

                                           tGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGAttt                                 <  1:3099311/57‑1 (MQ=255)

                                           tGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACa                   <  1:1790594/71‑1 (MQ=255)

                                            gagaTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAgta                                            >  1:5342075/1‑45 (MQ=255)

                                            gagaTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAgta                                            >  1:507627/1‑45 (MQ=255)

                                            gagaTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAgta                                            >  1:5420728/1‑45 (MQ=255)

                                            gagaTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAgta                                            >  1:5553697/1‑45 (MQ=255)

                                            gagaTAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTaaa                                               <  1:1035054/42‑1 (MQ=255)

                                             agaTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACt                                                  <  1:447934/38‑1 (MQ=21)

                                             agaTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACt                                                  <  1:1822730/38‑1 (MQ=21)

                                             agaTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACt                                                  <  1:2422257/38‑1 (MQ=21)

                                             agaTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGatat                  <  1:3291639/70‑1 (MQ=255)

                                             agaTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGatat                  <  1:3771237/70‑1 (MQ=255)

                                              gaTAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAg                >  1:3082007/1‑71 (MQ=255)

                                              gaTAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAg                <  1:265496/71‑1 (MQ=255)

                                               aTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTgaa                                               >  1:4833002/1‑39 (MQ=16)

                                               aTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGAt                                   <  1:3829380/51‑1 (MQ=255)

                                               aTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGAt                                   <  1:2419711/51‑1 (MQ=255)

                                               aTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGAt                                   <  1:2896687/51‑1 (MQ=255)

                                               aTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGAtata                 <  1:2225637/69‑1 (MQ=255)

                                               aTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGAtata                 >  1:3039509/1‑69 (MQ=255)

                                               aTAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAg                >  1:2010975/1‑70 (MQ=255)

                                                tAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGAtata                 <  1:5071689/68‑1 (MQ=255)

                                                tAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGt               <  1:4606702/70‑1 (MQ=255)

                                                tAGAGAATCAGCAGTGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTa              <  1:1399617/71‑1 (MQ=255)

                                                tAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTaa                                                <  1:2885980/37‑1 (MQ=255)

                                                tAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCTCAACGACATAGTa              <  1:1553546/71‑1 (MQ=255)

                                                tAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTa              <  1:5403146/71‑1 (MQ=255)

                                                tAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTACCAGATTTGCAGCGCAACGACATAGTa              <  1:4030605/71‑1 (MQ=255)

                                                 agagAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAg                                     >  1:4100467/1‑47 (MQ=255)

                                                 agagAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTa              >  1:136720/1‑70 (MQ=255)

                                                 agagAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTa              <  1:4914839/70‑1 (MQ=255)

                                                  gagaATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGata                   >  1:3129678/1‑62 (MQ=255)

                                                  gagaATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGata                   >  1:2970162/1‑62 (MQ=255)

                                                  gagaATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGt               >  1:4002479/1‑68 (MQ=255)

                                                  gagaATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGt               >  1:1350989/1‑68 (MQ=255)

                                                  gagaATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTata            <  1:700401/71‑3 (MQ=255)

                                                  gagaATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTat             <  1:700044/70‑2 (MQ=255)

                                                  gagaATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTat             <  1:5468723/70‑2 (MQ=255)

                                                  gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa            <  1:4845549/71‑1 (MQ=255)

                                                  gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa            <  1:4334378/71‑1 (MQ=255)

                                                  gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa            <  1:3796277/71‑1 (MQ=255)

                                                  gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa            <  1:2087903/71‑1 (MQ=255)

                                                  gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa            <  1:3352644/71‑1 (MQ=255)

                                                  gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa            >  1:328063/1‑71 (MQ=255)

                                                  gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa            <  1:2283969/71‑1 (MQ=255)

                                                  gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa            <  1:762769/71‑1 (MQ=255)

                                                    gaATCAGCAGGGCACGCATGCCGTAAAAGCTaaaa                                              <  1:1031592/35‑1 (MQ=255)

                                                     aaTCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATa                 >  1:1265823/1‑63 (MQ=255)

                                                     aaTCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATa                 >  1:4607506/1‑63 (MQ=255)

                                                      aTCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAg         <  1:1708427/70‑1 (MQ=255)

                                                        cagcagGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAgcgc      >  1:4318729/1‑71 (MQ=255)

                                                        cagcagGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAgcgc      >  1:2903474/1‑71 (MQ=255)

                                                         agcagGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAgcgcg     >  1:626790/1‑71 (MQ=255)

                                                          gcagGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa            >  1:494033/1‑63 (MQ=255)

                                                           cagGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTg                                <  1:2589670/42‑1 (MQ=255)

                                                            agGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACa                   <  1:283032/54‑1 (MQ=255)

                                                            agGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCgg  >  1:1929373/1‑71 (MQ=255)

                                                            agGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCgg  >  1:2968215/1‑71 (MQ=255)

AATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGG  >  Exported/454933‑455063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`