Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 539,419 C→T 8.9% G137D (GGC→GAC)  dps ← Fe‑binding and storage protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported539,4190CT 8.9% 220.3 / 9.0 78G137D (GGC→GAC) dpsFe‑binding and storage protein
Reads supporting (aligned to +/- strand):  ref base C (45/26);  new base T (5/2);  total (50/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTGA  >  Exported/539352‑539487
                                                                   |                                                                    
caGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCaa                                                                   >  1:4555206/1‑71 (MQ=255)
 aGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTTCGGTGTCGTCATCTTTCGCTTCGCCAAt                                                                  <  1:2582910/71‑1 (MQ=255)
   gAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCaa                                                                   <  1:3030321/68‑1 (MQ=255)
     aTTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGc                                                               <  1:3109696/70‑1 (MQ=255)
      tttATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGc                                                               <  1:3896860/69‑1 (MQ=255)
       ttATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCttt                                                            >  1:923255/1‑71 (MQ=255)
        tATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTg                                                           >  1:5672726/1‑71 (MQ=255)
         aTCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTg                                                           <  1:910395/70‑1 (MQ=255)
          tCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCg                                                         >  1:2403753/1‑71 (MQ=255)
           ccAGGTCGCGAGCCGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCg                                                         >  1:1132617/1‑70 (MQ=255)
           ccAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCg                                                         >  1:726002/1‑70 (MQ=255)
           ccAGGTCGCGAGACGCGGCGGTCAGGATATCTGAGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTg                                                           <  1:2506458/68‑1 (MQ=255)
              ggTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCtt                                                             >  1:1474810/1‑63 (MQ=255)
              ggTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCtt                                                             >  1:2639569/1‑63 (MQ=255)
               gTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGt                                                    >  1:3313902/1‑71 (MQ=255)
                tCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGc                                                               <  1:2600665/59‑1 (MQ=255)
                  gcgAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGTCAATCGCTTTGCGTACGTCAt                                                 >  1:3107115/1‑71 (MQ=255)
                  gcgAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGTCAATCGCTTTGCGTACGTCAt                                                 >  1:5066006/1‑71 (MQ=255)
                  gcgAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGTCAATCGCTTTGCGTACGTCAt                                                 >  1:2234694/1‑71 (MQ=255)
                  gcgAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGTCAATCGCTTTGCGTACGTCAt                                                 >  1:510868/1‑71 (MQ=255)
                  gcgAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGTCAATCGCGTTGCGTACGTCAt                                                 >  1:2384356/1‑71 (MQ=255)
                     agaCGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCg                                                                <  1:535143/53‑1 (MQ=255)
                     agaCGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCg                                                                <  1:135166/53‑1 (MQ=255)
                     agaCGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTAc                                                      >  1:1579285/1‑63 (MQ=255)
                     agaCGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTAc                                                      >  1:4175540/1‑63 (MQ=255)
                       aCGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGc                                             >  1:2403680/1‑70 (MQ=255)
                          cggcggTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGcga                                           >  1:1428012/1‑69 (MQ=255)
                            gcggTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGcgacga                                        <  1:3637281/70‑1 (MQ=255)
                              ggTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTTCGTCATTAGCGACGATTg                                     <  1:4727389/71‑1 (MQ=255)
                                tCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCg                                   <  1:3983064/71‑1 (MQ=255)
                                     atatCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGt                                                    >  1:2721352/1‑49 (MQ=255)
                                     atatCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTCTGCGTACGTCATTAGCGACGATTGCGTAACg                              >  1:2374323/1‑71 (MQ=255)
                                      tatCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACgg                             >  1:4666363/1‑71 (MQ=255)
                                      tatCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACgg                             >  1:4850918/1‑71 (MQ=255)
                                      tatCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACgg                             >  1:4935569/1‑71 (MQ=255)
                                       atCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGt                            >  1:3819150/1‑71 (MQ=255)
                                       atCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGt                            >  1:368412/1‑71 (MQ=255)
                                        tCTGCGGTGTCGTCATCTTTCGCTTCGTCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGt                            <  1:3032657/70‑1 (MQ=255)
                                        tCTGCGGTGTCGTCATCTTTCGCTTCGTCAATCGCTTTGCGTACGGCATTAGCGACGATTGCGTAACGGt                            <  1:4928398/70‑1 (MQ=255)
                                        tCTGCGGTGTCGTCATCTTTCCCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTaa                                >  1:35681/1‑66 (MQ=255)
                                         cTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAg                                              >  1:3917455/1‑51 (MQ=255)
                                         cTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAg                                              >  1:5309912/1‑51 (MQ=255)
                                         cTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAg                                              >  1:1141421/1‑51 (MQ=255)
                                         cTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGt                            >  1:3809052/1‑69 (MQ=255)
                                          tGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCac                         >  1:1157685/1‑70 (MQ=255)
                                          tGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAg                         >  1:1037903/1‑71 (MQ=255)
                                            cGGTGTCGTCTTCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGcc                       <  1:5540718/71‑1 (MQ=255)
                                            cGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGc                        >  1:5133631/1‑70 (MQ=255)
                                            cGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGc                        <  1:3153749/70‑1 (MQ=255)
                                              gtgtCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGt                                  <  1:1455351/58‑1 (MQ=255)
                                              gtgtCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAg                     >  1:3087210/1‑71 (MQ=255)
                                              gtgtCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAg                     <  1:921659/71‑1 (MQ=255)
                                              gtgtCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAg                     <  1:928229/71‑1 (MQ=255)
                                               tgtCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGt                    <  1:4299581/71‑1 (MQ=255)
                                                 tcgtcATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGtt                   <  1:3286185/70‑1 (MQ=255)
                                                 tcgtcATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTc                  >  1:2649588/1‑71 (MQ=255)
                                                   gtcATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCtt                <  1:5385388/71‑1 (MQ=255)
                                                   gtcATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCtt                <  1:3646846/71‑1 (MQ=255)
                                                   gtcATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCtt                <  1:142928/71‑1 (MQ=255)
                                                   gtcATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCtt                >  1:5567584/1‑71 (MQ=255)
                                                    tcATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCttt               >  1:2355326/1‑71 (MQ=255)
                                                    tcATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCtt                >  1:1465695/1‑70 (MQ=255)
                                                     cATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCttt               >  1:2341403/1‑70 (MQ=255)
                                                     cATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCttt               >  1:3973680/1‑70 (MQ=255)
                                                     cATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCttt               >  1:1238619/1‑70 (MQ=255)
                                                       tCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCttt               >  1:90834/1‑68 (MQ=255)
                                                       tCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCttt               >  1:1528452/1‑68 (MQ=255)
                                                       tCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCtt                >  1:740076/1‑67 (MQ=255)
                                                        cTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGAt                                       >  1:2241399/1‑43 (MQ=255)
                                                        cTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCa                          <  1:3843081/56‑1 (MQ=255)
                                                         tttCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTc                  <  1:2281582/63‑1 (MQ=255)
                                                         tttCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTc                  <  1:5358780/63‑1 (MQ=255)
                                                         tttCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTc                  <  1:3426584/63‑1 (MQ=255)
                                                         tttCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCa             >  1:884569/1‑68 (MQ=255)
                                                         tttCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCa             >  1:4504769/1‑68 (MQ=255)
                                                         tttCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGt          >  1:3043762/1‑71 (MQ=255)
                                                         tttCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGt          >  1:2587037/1‑71 (MQ=255)
                                                         tttCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGt          >  1:681726/1‑71 (MQ=255)
                                                          ttcgcttcgcCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAgg           >  1:3478876/1‑69 (MQ=255)
                                                          ttcgcttcgcCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGt          >  1:5284840/1‑70 (MQ=255)
                                                           tcgcttcgcCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAg            <  1:3708682/67‑1 (MQ=255)
                                                           tcgcttcgcCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGa        >  1:2970097/1‑71 (MQ=255)
                                                             gcttcgcCAATCGCTTTGCGTACGTCATTAGcgacg                                         <  1:5155492/36‑1 (MQ=255)
                                                             gcttcgcCAATCGCTTTGCGTACGTCATTAGcgacg                                         <  1:3776566/36‑1 (MQ=255)
                                                             gcttcgcCAATCGCTTTGCGTACGTCATTAGcgacg                                         <  1:2775001/36‑1 (MQ=255)
                                                             gcttcgcCAATCGCTTTGCGTACGTCATTAGcgacg                                         <  1:956576/36‑1 (MQ=255)
                                                             gcttcgcCAATCGCTTTGCGTACGTCATTAGcgacg                                         <  1:1697647/36‑1 (MQ=255)
                                                                tcgcCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAg            >  1:1038302/1‑62 (MQ=255)
                                                                tcgcCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCt    >  1:4647167/1‑70 (MQ=255)
                                                                tcgcCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCt    >  1:3633177/1‑70 (MQ=255)
                                                                 cgcCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTg   >  1:5687749/1‑70 (MQ=255)
                                                                 cgcCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTg   >  1:5153330/1‑70 (MQ=255)
                                                                 cgcCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTg   <  1:1836150/70‑1 (MQ=255)
                                                                  gcCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTGa  <  1:3577016/70‑1 (MQ=255)
                                                                   |                                                                    
CAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATATCTGCGGTGTCGTCATCTTTCGCTTCGCCAATCGCTTTGCGTACGTCATTAGCGACGATTGCGTAACGGTCAGCCAGTTCTTTCAGGTGATCCTGA  >  Exported/539352‑539487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: