Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	608,043	T→G	14.3%	A737A (GCA→GCC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	608,043	0	T	G	14.3%	148.3 / 21.8	70	A737A (GCA→GCC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base T (32/28);  new base G (4/6);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 5.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCA  >  Exported/607974‑608107                                                                       |                                                                 cATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACt                                                                  <  1:4815956/70‑1 (MQ=255)   tAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGGCAGCGAGTTGAAACGTACTGc                                                                <  1:3749529/70‑1 (MQ=255)    aGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCg                                                               >  1:3536577/1‑70 (MQ=255)       ttGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGc                                                           <  1:2617571/71‑1 (MQ=255)         gAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGAGAGTTGAAACGTACTGCGTAGcc                                                          <  1:83408/70‑1 (MQ=255)           gtaaaGGTACGTGAAATAACATCCTGTTGCT‑GTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGa                                                       <  1:350587/68‑1 (MQ=255)              aaGGTACGTGAAATAACATCCTGTTGCT‑GTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGaga                                                     <  1:2969174/70‑3 (MQ=255)              aaGGTACGTGAAATAACATCCTGTTGCT‑GTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGaga                                                     <  1:404675/70‑3 (MQ=255)              aaGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATa                                                     >  1:5315911/1‑70 (MQ=255)              aaGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATa                                                     >  1:227011/1‑70 (MQ=255)                  tACGTGAAATAACATCCTGTTGCT‑GTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGacac                                                  >  1:3279435/1‑69 (MQ=255)                  tACGTGAAATAACATCCTGTTGCT‑GTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                                 >  1:398507/1‑70 (MQ=255)                      aGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACTGATgg                                            <  1:87811/71‑1 (MQ=255)                      aGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATgg                                            <  1:3814349/71‑1 (MQ=255)                        taataaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACgg                                                <  1:5158368/65‑1 (MQ=255)                        taataaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACgg                                                <  1:3326983/65‑1 (MQ=255)                        taataaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACg                                                 <  1:366473/64‑1 (MQ=255)                         aataaCATCCTGTTGCT‑GTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGCATTGTCa                                         <  1:569736/71‑1 (MQ=255)                          ataaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt                                              >  1:3593189/1‑65 (MQ=255)                          ataaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt                                              >  1:531529/1‑65 (MQ=255)                          ataaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt                                              >  1:5538430/1‑65 (MQ=255)                          ataaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt                                              >  1:2715948/1‑65 (MQ=255)                          ataaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg                                        <  1:472346/71‑1 (MQ=255)                          ataaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg                                        >  1:1064665/1‑71 (MQ=255)                          ataaCATCCTGTTGCT‑GTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAg                                        >  1:4040379/1‑71 (MQ=255)                           taaCATCCTGTTGCT‑GTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCa                                         <  1:3450180/69‑1 (MQ=255)                            aaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAg                                                            <  1:1961296/49‑1 (MQ=255)                             aCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGt                                                              >  1:1115134/1‑46 (MQ=255)                             aCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACgg                                                >  1:2975706/1‑60 (MQ=255)                             aCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCa                                         >  1:2340301/1‑67 (MQ=255)                               gTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCg                                   >  1:5527442/1‑71 (MQ=255)                               gTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCg                                   >  1:3563115/1‑71 (MQ=255)                                tCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                                           <  1:5225139/62‑1 (MQ=255)                                  ctgctgct‑gTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCa                                         >  1:753132/1‑62 (MQ=255)                                  ctgctgct‑gTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa                                 >  1:2966344/1‑70 (MQ=255)                                  ctgctgct‑gTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa                                 >  1:1178530/1‑70 (MQ=255)                                  ctgctgct‑gTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa                                 >  1:3375138/1‑70 (MQ=255)                                  ctgctgca‑gTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa                                 >  1:562899/1‑70 (MQ=255)                                   tgctgct‑gTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt                                      <  1:1232043/64‑1 (MQ=255)                                     ctgctgGTTCTTTAGTCAGCGAGTTGAAACGTACTGc                                                                >  1:4867945/1‑37 (MQ=38)                                        ct‑gTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGcc                                                          <  1:1052531/39‑1 (MQ=255)                                        ct‑gTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttttt                          <  1:1119205/71‑1 (MQ=255)                                            ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc                        <  1:1488244/70‑1 (MQ=255)                                             tCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCgg                      >  1:2144533/1‑71 (MQ=255)                                             tCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCgg                      >  1:5385737/1‑71 (MQ=255)                                             tCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCgg                      >  1:3291109/1‑71 (MQ=255)                                              cTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGa                                                       >  1:5045808/1‑37 (MQ=255)                                              cTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGa                                                       >  1:4501310/1‑37 (MQ=255)                                              cTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATa                                                     >  1:2851405/1‑39 (MQ=255)                                              cTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATa                                                     >  1:2000940/1‑39 (MQ=255)                                              cTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg                                        >  1:4205704/1‑52 (MQ=255)                                                  aGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttttt                          <  1:5332446/62‑1 (MQ=255)                                                   gTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCgg                      >  1:3904211/1‑65 (MQ=9)                                                   gTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTc                <  1:2381112/71‑1 (MQ=255)                                                   gTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTc                >  1:4713787/1‑71 (MQ=255)                                                    tCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTcc               <  1:4341197/71‑1 (MQ=255)                                                    tCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTcc               <  1:1067665/71‑1 (MQ=255)                                                      aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGtt                   <  1:5478999/65‑1 (MQ=255)                                                      aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGtt                   <  1:862411/65‑1 (MQ=255)                                                      aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCAt             >  1:4465790/1‑71 (MQ=255)                                                       gCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                                           <  1:2426693/40‑1 (MQ=255)                                                        cGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATcg           >  1:2599153/1‑71 (MQ=255)                                                          cgTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTcc                        <  1:5500462/55‑1 (MQ=12)                                                            ttGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTc                         <  1:1169838/53‑1 (MQ=255)                                                            ttGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTc                         <  1:1855915/53‑1 (MQ=255)                                                              gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCa              >  1:3090896/1‑62 (MQ=255)                                                              gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCg      <  1:1841906/70‑1 (MQ=255)                                                                aaCGTACTGCGTAGCCAGATACAGGGATGGTCAGCTGCGGATATTTTTcc                        <  1:4784597/50‑1 (MQ=255)                                                                 aCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGagca  <  1:2574704/71‑1 (MQ=255)                                                                 aCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGag    >  1:4723853/1‑69 (MQ=255)                                                                    tACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttt                            <  1:1550897/42‑1 (MQ=255)                                                                       |                                                                 CATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCA  >  Exported/607974‑608107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑