Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	608,090	T→G	42.4%	N722H (AAC→CAC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	608,090	0	T	G	42.4%	50.5 / 122.2	92	N722H (AAC→CAC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base T (35/18);  new base G (25/14);  total (60/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

GTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGA  >  Exported/608023‑608159                                                                    |                                                                      gTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTc                                                                    <  1:2381112/71‑1 (MQ=255) gTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTc                                                                    >  1:4713787/1‑71 (MQ=255)  tCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTcc                                                                   <  1:4341197/71‑1 (MQ=255)  tCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTcc                                                                   <  1:1067665/71‑1 (MQ=255)    aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGtt                                                                       <  1:862411/65‑1 (MQ=255)    aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGtt                                                                       <  1:5478999/65‑1 (MQ=255)    aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCAt                                                                 >  1:4465790/1‑71 (MQ=255)      cGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATcg                                                               >  1:2599153/1‑71 (MQ=255)            gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCa                                                                  >  1:3090896/1‑62 (MQ=255)            gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCg                                                          <  1:1841906/70‑1 (MQ=255)               aCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGagca                                                      <  1:2574704/71‑1 (MQ=255)               aCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGag                                                        >  1:4723853/1‑69 (MQ=255)                    cggCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAtt                                                 >  1:2496566/3‑71 (MQ=12)                    cggCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAt                                                  >  1:2145640/3‑70 (MQ=12)                      gCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTc                                               >  1:3894100/1‑71 (MQ=16)                       cGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCAt                                                                 >  1:4987806/1‑52 (MQ=255)                       cGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAttt                                                <  1:2534990/69‑1 (MQ=255)                       cGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCa                                              >  1:686846/1‑71 (MQ=255)                          aGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCga                                           >  1:611247/1‑70 (MQ=12)                           gCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCgat                                          >  1:204410/1‑69 (MQ=12)                              agagACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATtc                                        <  1:3476824/70‑1 (MQ=9)                              aGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtca                                       >  1:4707475/1‑71 (MQ=255)                               gagacacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATtcat                                      <  1:3047823/68‑1 (MQ=9)                               gagacacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATtcat                                      <  1:1208217/68‑1 (MQ=9)                               gagacacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATtcat                                      <  1:1890052/68‑1 (MQ=9)                                 tACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcatc                                     <  1:4459557/70‑1 (MQ=255)                                 tACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcatc                                     <  1:3986411/70‑1 (MQ=255)                                 gacacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATtcatta                                    >  1:2583400/2‑69 (MQ=9)                                  acacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtc                                        >  1:2752349/1‑66 (MQ=255)                                  acacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc                                   >  1:378263/1‑71 (MQ=255)                                  acacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc                                   >  1:125761/1‑71 (MQ=255)                                   cacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACg                                  <  1:2878476/71‑1 (MQ=12)                                   cacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACg                                  <  1:2610403/71‑1 (MQ=12)                                   cacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACg                                  <  1:3994210/71‑1 (MQ=12)                                   cacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACg                                  <  1:5515929/71‑1 (MQ=12)                                   cacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACg                                  >  1:1900190/1‑71 (MQ=255)                                     cGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGtt                                <  1:724340/71‑1 (MQ=255)                                      ggATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGtt                                >  1:2256425/1‑70 (MQ=255)                                       gATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTaa                              >  1:3215964/1‑71 (MQ=255)                                           gTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTaa                              <  1:388756/67‑1 (MQ=255)                                           gTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGt                           <  1:623050/70‑1 (MQ=255)                                             cAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcatc                                     >  1:3268764/1‑58 (MQ=255)                                             cAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt                          >  1:672858/1‑69 (MQ=255)                                              aGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTaa                              >  1:5346504/1‑64 (MQ=255)                                              aGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAg                       >  1:4238926/1‑71 (MQ=255)                                                 ttaggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTg                    <  1:430491/68‑1 (MQ=255)                                                   cGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTg                    <  1:3561424/69‑1 (MQ=255)                                                   cGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCt                  >  1:4815554/1‑71 (MQ=255)                                                   cGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCt                  <  1:2347595/71‑1 (MQ=255)                                                    ggATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCa                        <  1:684597/64‑1 (MQ=255)                                                    ggATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTg                 <  1:4759502/71‑1 (MQ=255)                                                    ggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTgtt                  <  1:1529062/70‑3 (MQ=255)                                                     gATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGa                >  1:352683/1‑71 (MQ=12)                                                     gATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGa                >  1:5338729/1‑71 (MQ=12)                                                      atatTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTg                    <  1:5669114/66‑1 (MQ=255)                                                       tatTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcat                                      >  1:3001888/1‑47 (MQ=255)                                                       tatTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGAc                             >  1:28388/1‑56 (MQ=255)                                                       tatTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGacc              >  1:3784295/1‑71 (MQ=12)                                                        atTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCt                  >  1:223045/1‑66 (MQ=255)                                                        atTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGacca             >  1:762954/1‑71 (MQ=255)                                                        atTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGaccg             >  1:4047357/1‑70 (MQ=255)                                                        atTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGaccg             >  1:2994233/1‑70 (MQ=255)                                                        atTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGaccg             >  1:3157662/1‑70 (MQ=255)                                                        atTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGacc              >  1:4518422/1‑70 (MQ=255)                                                         tttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGtt                                         >  1:4697980/1‑42 (MQ=255)                                                         tttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCa                        >  1:1148539/1‑59 (MQ=255)                                                         tttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGaccac            >  1:4866991/1‑71 (MQ=255)                                                         tttttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGacc              <  1:1020302/69‑1 (MQ=255)                                                          ttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCa                                              <  1:659991/36‑1 (MQ=255)                                                          ttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCAc                                             >  1:1687938/1‑37 (MQ=255)                                                          ttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCAc                                             >  1:4531187/1‑37 (MQ=255)                                                          ttttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGaccgcc           >  1:2690368/1‑71 (MQ=255)                                                           tttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACg                                            >  1:5128369/1‑37 (MQ=255)                                                           tttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTg                 >  1:1028818/1‑64 (MQ=255)                                                           tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTgt                   >  1:4919348/1‑61 (MQ=255)                                                           tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTgt                   >  1:2252157/1‑61 (MQ=255)                                                           tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGaccgcc           <  1:41402/70‑1 (MQ=255)                                                           tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGaccgcc           <  1:1711094/70‑1 (MQ=255)                                                           tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCt          >  1:4862636/1‑71 (MQ=255)                                                           tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCt          >  1:4776960/1‑71 (MQ=255)                                                           tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCt          >  1:3596185/1‑71 (MQ=255)                                                           tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCt          >  1:5486463/1‑71 (MQ=255)                                                            ttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACg                                            >  1:4524001/1‑36 (MQ=255)                                                            ttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACg                                            >  1:3913732/1‑36 (MQ=255)                                                            ttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcat                                      >  1:422474/1‑42 (MQ=255)                                                            ttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcat                                      >  1:2108252/1‑42 (MQ=255)                                                             tCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTc        >  1:894824/1‑71 (MQ=12)                                                             tCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTc        >  1:4702231/1‑71 (MQ=12)                                                             tCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTc        >  1:2940340/1‑71 (MQ=12)                                                             tCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTc        >  1:2933824/1‑71 (MQ=12)                                                              ccGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGtt                                <  1:3789738/46‑1 (MQ=255)                                                              ccGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTc        <  1:1375712/70‑1 (MQ=255)                                                                 ggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGa                >  1:4418540/1‑59 (MQ=255)                                                                 ggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGa                >  1:3606674/1‑59 (MQ=255)                                                                   ttttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatgga  >  1:4004942/1‑71 (MQ=255)                                                                    |                                                                      GTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGA  >  Exported/608023‑608159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑