Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	608,097	C→G	42.4%	A719A (GCG→GCC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	608,097	0	C	G	42.4%	81.8 / 104.8	92	A719A (GCG→GCC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base C (37/16);  new base G (25/14);  total (62/30)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

CGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCT  >  Exported/608028‑608163                                                                      |                                                                   cGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATcg                                                                   >  1:2599153/1‑71 (MQ=255)       gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCg                                                              <  1:1841906/70‑1 (MQ=255)          aCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGagca                                                          <  1:2574704/71‑1 (MQ=255)          aCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGag                                                            >  1:4723853/1‑69 (MQ=255)               cggCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAtt                                                     >  1:2496566/3‑71 (MQ=12)               cggCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAt                                                      >  1:2145640/3‑70 (MQ=12)                 gCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTc                                                   >  1:3894100/1‑71 (MQ=16)                  cGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAttt                                                    <  1:2534990/69‑1 (MQ=255)                  cGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCa                                                  >  1:686846/1‑71 (MQ=255)                     aGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCga                                               >  1:611247/1‑70 (MQ=12)                      gCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCgat                                              >  1:204410/1‑69 (MQ=12)                         agagACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATtc                                            <  1:3476824/70‑1 (MQ=9)                         aGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtca                                           >  1:4707475/1‑71 (MQ=255)                          gagacacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATtcat                                          <  1:1208217/68‑1 (MQ=9)                          gagacacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATtcat                                          <  1:1890052/68‑1 (MQ=9)                          gagacacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATtcat                                          <  1:3047823/68‑1 (MQ=9)                            tACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcatc                                         <  1:4459557/70‑1 (MQ=255)                            tACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcatc                                         <  1:3986411/70‑1 (MQ=255)                            gacacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATtcatta                                        >  1:2583400/2‑69 (MQ=9)                             acacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtc                                            >  1:2752349/1‑66 (MQ=255)                             acacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc                                       >  1:378263/1‑71 (MQ=255)                             acacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc                                       >  1:125761/1‑71 (MQ=255)                              cacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACg                                      <  1:3994210/71‑1 (MQ=12)                              cacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACg                                      <  1:2610403/71‑1 (MQ=12)                              cacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACg                                      <  1:5515929/71‑1 (MQ=12)                              cacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACg                                      <  1:2878476/71‑1 (MQ=12)                              cacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACg                                      >  1:1900190/1‑71 (MQ=255)                                cGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGtt                                    <  1:724340/71‑1 (MQ=255)                                 ggATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGtt                                    >  1:2256425/1‑70 (MQ=255)                                  gATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTaa                                  >  1:3215964/1‑71 (MQ=255)                                      gTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTaa                                  <  1:388756/67‑1 (MQ=255)                                      gTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGt                               <  1:623050/70‑1 (MQ=255)                                        cAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcatc                                         >  1:3268764/1‑58 (MQ=255)                                        cAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt                              >  1:672858/1‑69 (MQ=255)                                         aGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTaa                                  >  1:5346504/1‑64 (MQ=255)                                         aGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAg                           >  1:4238926/1‑71 (MQ=255)                                            ttaggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTg                        <  1:430491/68‑1 (MQ=255)                                              cGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTg                        <  1:3561424/69‑1 (MQ=255)                                              cGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCt                      <  1:2347595/71‑1 (MQ=255)                                              cGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCt                      >  1:4815554/1‑71 (MQ=255)                                               ggATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCa                            <  1:684597/64‑1 (MQ=255)                                               ggATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTg                     <  1:4759502/71‑1 (MQ=255)                                               ggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTgtt                      <  1:1529062/70‑3 (MQ=255)                                                gATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGa                    >  1:5338729/1‑71 (MQ=12)                                                gATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGa                    >  1:352683/1‑71 (MQ=12)                                                 atatTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTg                        <  1:5669114/66‑1 (MQ=255)                                                  tatTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcat                                          >  1:3001888/1‑47 (MQ=255)                                                  tatTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGAc                                 >  1:28388/1‑56 (MQ=255)                                                  tatTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGacc                  >  1:3784295/1‑71 (MQ=12)                                                   atTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCt                      >  1:223045/1‑66 (MQ=255)                                                   atTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGacca                 >  1:762954/1‑71 (MQ=255)                                                   atTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGaccg                 >  1:4047357/1‑70 (MQ=255)                                                   atTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGaccg                 >  1:3157662/1‑70 (MQ=255)                                                   atTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGaccg                 >  1:2994233/1‑70 (MQ=255)                                                   atTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGacc                  >  1:4518422/1‑70 (MQ=255)                                                    tttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGtt                                             >  1:4697980/1‑42 (MQ=255)                                                    tttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCa                            >  1:1148539/1‑59 (MQ=255)                                                    tttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGaccac                >  1:4866991/1‑71 (MQ=255)                                                    tttttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGacc                  <  1:1020302/69‑1 (MQ=255)                                                     ttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCa                                                  <  1:659991/36‑1 (MQ=255)                                                     ttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCAc                                                 >  1:1687938/1‑37 (MQ=255)                                                     ttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCAc                                                 >  1:4531187/1‑37 (MQ=255)                                                     ttttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGaccgcc               >  1:2690368/1‑71 (MQ=255)                                                      tttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACg                                                >  1:5128369/1‑37 (MQ=255)                                                      tttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTg                     >  1:1028818/1‑64 (MQ=255)                                                      tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTgt                       >  1:4919348/1‑61 (MQ=255)                                                      tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTgt                       >  1:2252157/1‑61 (MQ=255)                                                      tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGaccgcc               <  1:41402/70‑1 (MQ=255)                                                      tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGaccgcc               <  1:1711094/70‑1 (MQ=255)                                                      tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCt              >  1:4776960/1‑71 (MQ=255)                                                      tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCt              >  1:4862636/1‑71 (MQ=255)                                                      tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCt              >  1:3596185/1‑71 (MQ=255)                                                      tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCt              >  1:5486463/1‑71 (MQ=255)                                                       ttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACg                                                >  1:4524001/1‑36 (MQ=255)                                                       ttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACg                                                >  1:3913732/1‑36 (MQ=255)                                                       ttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcat                                          >  1:422474/1‑42 (MQ=255)                                                       ttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcat                                          >  1:2108252/1‑42 (MQ=255)                                                        tCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTc            >  1:2933824/1‑71 (MQ=12)                                                        tCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTc            >  1:2940340/1‑71 (MQ=12)                                                        tCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTc            >  1:894824/1‑71 (MQ=12)                                                        tCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTc            >  1:4702231/1‑71 (MQ=12)                                                         ccGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGtt                                    <  1:3789738/46‑1 (MQ=255)                                                         ccGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTc            <  1:1375712/70‑1 (MQ=255)                                                            ggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGa                    >  1:3606674/1‑59 (MQ=255)                                                            ggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGa                    >  1:4418540/1‑59 (MQ=255)                                                              ttttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatgga      >  1:4004942/1‑71 (MQ=255)                                                                ttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCga          >  1:1784602/1‑65 (MQ=255)                                                                 tCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgat         >  1:1564142/1‑65 (MQ=255)                                                                 tCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgat         >  1:1499616/1‑65 (MQ=255)                                                                  ccATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCt  >  1:2694238/1‑71 (MQ=255)                                                                  ccATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCt  >  1:1309634/1‑71 (MQ=255)                                                                  ccATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCt  >  1:1071379/1‑71 (MQ=255)                                                                     tCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTc                             <  1:574831/41‑1 (MQ=255)                                                                      cgcgTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCga          >  1:916362/1‑59 (MQ=255)                                                                      |                                                                   CGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCT  >  Exported/608028‑608163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑