BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	608,103	G→C	38.6%	L717L (CTC→CTG)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	608,103	0	G	C	38.6%	113.1 / 104.3	101	L717L (CTC→CTG)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base G (42/20); new base C (25/14); total (67/34)
Fisher's exact test for biased strand distribution p-value = 8.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

Only 100 of 108 total aligned reads displayed.

GAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGG  >  Exported/608034‑608172

gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCg                                                                       <  1:1841906/70‑1 (MQ=255)

   aCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGagca                                                                   <  1:2574704/71‑1 (MQ=255)

   aCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGag                                                                     >  1:4723853/1‑69 (MQ=255)

        cggCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAtt                                                              >  1:2496566/3‑71 (MQ=12)

        cggCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAt                                                               >  1:2145640/3‑70 (MQ=12)

          gCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTc                                                            >  1:3894100/1‑71 (MQ=16)

           cGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAttt                                                             <  1:2534990/69‑1 (MQ=255)

           cGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCa                                                           >  1:686846/1‑71 (MQ=255)

              aGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCga                                                        >  1:611247/1‑70 (MQ=12)

               gCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCgat                                                       >  1:204410/1‑69 (MQ=12)

                  agagACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATtc                                                     <  1:3476824/70‑1 (MQ=9)

                   gagacacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATtcat                                                   <  1:3047823/68‑1 (MQ=9)

                   gagacacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATtcat                                                   <  1:1208217/68‑1 (MQ=9)

                   gagacacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATtcat                                                   <  1:1890052/68‑1 (MQ=9)

                     tACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcatc                                                  <  1:4459557/70‑1 (MQ=255)

                     tACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcatc                                                  <  1:3986411/70‑1 (MQ=255)

                     gacacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATtcatta                                                 >  1:2583400/2‑69 (MQ=9)

                      acacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtc                                                     >  1:2752349/1‑66 (MQ=255)

                      acacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc                                                >  1:125761/1‑71 (MQ=255)

                      acacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc                                                >  1:378263/1‑71 (MQ=255)

                       cacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACg                                               <  1:2878476/71‑1 (MQ=12)

                       cacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACg                                               <  1:2610403/71‑1 (MQ=12)

                       cacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACg                                               <  1:3994210/71‑1 (MQ=12)

                       cacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACg                                               >  1:1900190/1‑71 (MQ=255)

                         cGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGtt                                             <  1:724340/71‑1 (MQ=255)

                           gATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTaa                                           >  1:3215964/1‑71 (MQ=255)

                               gTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTaa                                           <  1:388756/67‑1 (MQ=255)

                               gTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGt                                        <  1:623050/70‑1 (MQ=255)

                                 cAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcatc                                                  >  1:3268764/1‑58 (MQ=255)

                                 cAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt                                       >  1:672858/1‑69 (MQ=255)

                                  aGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTaa                                           >  1:5346504/1‑64 (MQ=255)

                                  aGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAg                                    >  1:4238926/1‑71 (MQ=255)

                                     ttaggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTg                                 <  1:430491/68‑1 (MQ=255)

                                       cGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCt                               <  1:2347595/71‑1 (MQ=255)

                                       cGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCt                               >  1:4815554/1‑71 (MQ=255)

                                        ggATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCa                                     <  1:684597/64‑1 (MQ=255)

                                        ggATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTg                              <  1:4759502/71‑1 (MQ=255)

                                        ggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTgtt                               <  1:1529062/70‑3 (MQ=255)

                                         gATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGa                             >  1:352683/1‑71 (MQ=12)

                                         gATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGa                             >  1:5338729/1‑71 (MQ=12)

                                          atatTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTg                                 <  1:5669114/66‑1 (MQ=255)

                                           tatTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcat                                                   >  1:3001888/1‑47 (MQ=255)

                                           tatTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGAc                                          >  1:28388/1‑56 (MQ=255)

                                           tatTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGacc                           >  1:3784295/1‑71 (MQ=12)

                                            atTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCt                               >  1:223045/1‑66 (MQ=255)

                                            atTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGacca                          >  1:762954/1‑71 (MQ=255)

                                            atTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGaccg                          >  1:2994233/1‑70 (MQ=255)

                                            atTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGaccg                          >  1:3157662/1‑70 (MQ=255)

                                            atTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGaccg                          >  1:4047357/1‑70 (MQ=255)

                                            atTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGacc                           >  1:4518422/1‑70 (MQ=255)

                                             tttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGtt                                                      >  1:4697980/1‑42 (MQ=255)

                                             tttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCa                                     >  1:1148539/1‑59 (MQ=255)

                                             tttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGaccac                         >  1:4866991/1‑71 (MQ=255)

                                             tttttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGacc                           <  1:1020302/69‑1 (MQ=255)

                                              ttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCa                                                           <  1:659991/36‑1 (MQ=255)

                                              ttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCAc                                                          >  1:4531187/1‑37 (MQ=255)

                                              ttttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGaccgcc                        >  1:2690368/1‑71 (MQ=255)

                                               tttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACg                                                         >  1:5128369/1‑37 (MQ=255)

                                               tttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTg                              >  1:1028818/1‑64 (MQ=255)

                                               tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTgt                                >  1:2252157/1‑61 (MQ=255)

                                               tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTgt                                >  1:4919348/1‑61 (MQ=255)

                                               tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGaccgcc                        <  1:1711094/70‑1 (MQ=255)

                                               tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGaccgcc                        <  1:41402/70‑1 (MQ=255)

                                               tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCt                       >  1:3596185/1‑71 (MQ=255)

                                               tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCt                       >  1:4776960/1‑71 (MQ=255)

                                               tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCt                       >  1:5486463/1‑71 (MQ=255)

                                               tttCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCt                       >  1:4862636/1‑71 (MQ=255)

                                                ttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACg                                                         >  1:4524001/1‑36 (MQ=255)

                                                ttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACg                                                         >  1:3913732/1‑36 (MQ=255)

                                                ttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcat                                                   >  1:422474/1‑42 (MQ=255)

                                                ttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcat                                                   >  1:2108252/1‑42 (MQ=255)

                                                 tCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTc                     >  1:894824/1‑71 (MQ=12)

                                                 tCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTc                     >  1:4702231/1‑71 (MQ=12)

                                                 tCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTc                     >  1:2933824/1‑71 (MQ=12)

                                                 tCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTc                     >  1:2940340/1‑71 (MQ=12)

                                                  ccGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGtt                                             <  1:3789738/46‑1 (MQ=255)

                                                  ccGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTc                     <  1:1375712/70‑1 (MQ=255)

                                                     ggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGa                             >  1:3606674/1‑59 (MQ=255)

                                                     ggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGa                             >  1:4418540/1‑59 (MQ=255)

                                                       ttttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatgga               >  1:4004942/1‑71 (MQ=255)

                                                         ttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCga                   >  1:1784602/1‑65 (MQ=255)

                                                          tCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgat                  >  1:1499616/1‑65 (MQ=255)

                                                          tCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgat                  >  1:1564142/1‑65 (MQ=255)

                                                           ccATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCt           >  1:1309634/1‑71 (MQ=255)

                                                           ccATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCt           >  1:2694238/1‑71 (MQ=255)

                                                           ccATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCt           >  1:1071379/1‑71 (MQ=255)

                                                              tCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTc                                      <  1:574831/41‑1 (MQ=255)

                                                                gcgTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtg      >  1:5125089/1‑71 (MQ=255)

                                                                gcgTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgt       <  1:4659684/70‑1 (MQ=255)

                                                                  gTCGAGAAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtggt    <  1:2142944/71‑1 (MQ=255)

                                                                   tCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGt                                  <  1:4758452/40‑1 (MQ=255)

                                                                   tCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTc                     <  1:5622409/53‑1 (MQ=255)

                                                                    cGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtggtg   >  1:229330/1‑70 (MQ=255)

                                                                    cGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtggtg   >  1:1158104/1‑70 (MQ=255)

                                                                     gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGtggtgg  <  1:2140849/70‑1 (MQ=255)

                                                                     gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGtggtgg  <  1:2369404/70‑1 (MQ=255)

                                                                     gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGtggtgg  <  1:2385207/70‑1 (MQ=255)

                                                                     gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGtggtgg  <  1:3282078/70‑1 (MQ=255)

                                                                     gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGtggtgg  <  1:1019380/70‑1 (MQ=255)

                                                                     gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGtggtgg  <  1:4259562/70‑1 (MQ=255)

GAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGG  >  Exported/608034‑608172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`