Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	608,340	G→A	30.9%	H638H (CAC→CAT)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	608,340	0	G	A	30.9%	73.5 / 43.1	62	H638H (CAC→CAT)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base G (26/16);  new base A (7/12);  total (34/28)
Fisher's exact test for biased strand distribution p-value = 9.72e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.07e-01

TTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCT  >  Exported/608272‑608410                                                                     |                                                                       ttAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGt                                                                       >  1:3836571/1‑70 (MQ=255) ttAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTg                                                                      >  1:1192396/1‑71 (MQ=255) ttAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCCTg                                                                      >  1:4201025/1‑71 (MQ=255)  tAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGt                                                                       <  1:2513253/69‑1 (MQ=255)  tAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTg                                                                      >  1:3385579/1‑70 (MQ=255)     cGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATc                                                                  <  1:3595044/71‑1 (MQ=255)      gTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCg                                                                 >  1:5471197/1‑71 (MQ=255)       tAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCg                                                                 <  1:2509113/70‑1 (MQ=255)         aGCAAACGGCAGTTTAGAAACGGAAGTCAGAGAGGCTACTCCACCTTTCTGGTAACGACCGTGCATCGGGt                                                              >  1:4693906/1‑71 (MQ=255)             aaCGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGc                                                          >  1:2058665/1‑71 (MQ=255)              aCGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGcg                                                         >  1:3138578/1‑70 (MQ=255)              aCGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGcg                                                         <  1:945225/71‑2 (MQ=255)              aCGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGcg                                                         >  1:321985/1‑70 (MQ=255)              aCGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGcg                                                         >  1:2844125/1‑70 (MQ=255)              aCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGc                                                          >  1:1711072/1‑70 (MQ=255)                ggCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGc                                                                     <  1:2386775/57‑1 (MQ=255)                ggCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGc                                                                     <  1:3407023/57‑1 (MQ=255)                 gCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGccc                                                      <  1:1432672/71‑1 (MQ=255)                   aGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCgg                                                    <  1:1762056/71‑1 (MQ=255)                   aGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCgg                                                    <  1:3073683/71‑1 (MQ=255)                         gCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGaa                                              <  1:2120626/71‑1 (MQ=255)                             cGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgc                                          <  1:5113031/71‑1 (MQ=255)                              ggAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCAccc                                                      >  1:2368787/1‑58 (MQ=255)                              ggAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCAccc                                                      >  1:1922208/1‑58 (MQ=255)                               gAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcgc                                        <  1:3688099/71‑1 (MQ=255)                                 aGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcgcgc                                      >  1:980134/1‑71 (MQ=255)                                 aGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcgcgc                                      >  1:3105067/1‑71 (MQ=255)                                   tCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGt                                                              <  1:5463821/45‑1 (MQ=255)                                       tgagGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGtt                                                             <  1:900613/41‑1 (MQ=255)                                       agagGCTACTGCACCTTTCTGGTCACGACCGTGCAt                                                                   >  1:2995951/1‑36 (MQ=255)                                       agagGCTACTGCACCTTTCTGGTCACGACCGTGCAt                                                                   >  1:3128837/1‑36 (MQ=255)                                       agagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                <  1:1399283/71‑1 (MQ=255)                                       agagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                <  1:2490337/71‑1 (MQ=255)                                          ggCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacga                              >  1:2161674/1‑70 (MQ=255)                                           gCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacgacg                            <  1:3342709/71‑1 (MQ=255)                                            cTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAg                                                           <  1:1676881/39‑1 (MQ=255)                                               cggCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCg                        >  1:616796/3‑71 (MQ=255)                                                ggCACCTTTGAGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCg                        <  1:276826/69‑1 (MQ=255)                                                 gCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacga                              >  1:3639095/1‑63 (MQ=255)                                                 gCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacga                              >  1:3379603/1‑63 (MQ=255)                                                 gCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACgacgac                          >  1:933964/1‑67 (MQ=255)                                                  cACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACgacgac                          >  1:5403555/1‑66 (MQ=255)                                                  cACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCt                     >  1:5464753/1‑71 (MQ=255)                                                  cACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCt                     >  1:5213759/1‑71 (MQ=255)                                                    ccTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCttg                   <  1:2552236/71‑3 (MQ=255)                                                       ttCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAg                                   >  1:2966184/1‑52 (MQ=255)                                                       ttCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGgt                >  1:4138635/1‑71 (MQ=255)                                                       ttCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGgt                >  1:2646935/1‑71 (MQ=255)                                                        tgcggTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCgt                <  1:1831786/67‑1 (MQ=255)                                                        tCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGgt                <  1:2347125/70‑1 (MQ=255)                                                         cTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacga                              >  1:2409864/1‑55 (MQ=255)                                                            gTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGgtgt              <  1:502969/68‑1 (MQ=255)                                                            gTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTAc           >  1:5106630/1‑71 (MQ=255)                                                            gTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTAc           >  1:3947601/1‑71 (MQ=255)                                                            gTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTAc           <  1:1035538/71‑1 (MQ=255)                                                             tCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCgta               >  1:4677054/1‑65 (MQ=255)                                                               aCGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTggg                  >  1:928333/1‑61 (MQ=255)                                                               aCGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTAccgg        >  1:4037640/1‑69 (MQ=255)                                                               aCGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTAccg         <  1:2079948/70‑2 (MQ=255)                                                                 gACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGtt      <  1:3841163/71‑1 (MQ=255)                                                                 gACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGtt      <  1:1515024/71‑1 (MQ=255)                                                                  aCCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGttt     <  1:4754005/71‑1 (MQ=255)                                                                     gTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCt  <  1:2869956/71‑1 (MQ=255)                                                                     |                                                                       TTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCT  >  Exported/608272‑608410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑