Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	608,460	A→G	8.0%	A598A (GCT→GCC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	608,460	0	A	G	8.0%	209.4 / 7.0	75	A598A (GCT→GCC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base A (33/36);  new base G (3/3);  total (36/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

TTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTC  >  Exported/608398‑608528                                                               |                                                                     ttACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACg                                                              >  1:4274323/1‑71 (MQ=255)  tACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGt                                                                  <  1:3955736/66‑1 (MQ=255)   aCCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACgg                                                             >  1:115970/1‑70 (MQ=255)       gTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACgg                                                             >  1:1062633/1‑66 (MQ=255)       gTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACgg                                                             >  1:3634530/1‑66 (MQ=255)       gTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGgt                                                        >  1:4710746/1‑71 (MQ=255)       gTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGgt                                                        >  1:2879597/1‑71 (MQ=255)       gTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGgt                                                        >  1:2541054/1‑71 (MQ=255)       gTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGgt                                                        >  1:2102100/1‑71 (MQ=255)        ttttCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCAc                                                               <  1:4317281/63‑1 (MQ=255)          ttCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGgtgt                                                      <  1:4831545/70‑1 (MQ=255)          ttCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGtgtg                                                     >  1:1910693/1‑71 (MQ=255)          ttCTTACCATACACAACGTTAGAAGAGATGGTCAGAACAGACTGAGTCGGGATAg                                                                     >  1:3934166/1‑55 (MQ=255)               aCCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCa                                                   <  1:3568046/68‑1 (MQ=255)               aCCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGt                                                 >  1:1920407/1‑70 (MQ=255)               aCCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGt                                                 <  1:4449503/70‑1 (MQ=255)                ccATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATATCGTCACGGTAGGTGTGCAGttt                                               <  1:82175/71‑1 (MQ=255)                ccATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAg                                                  <  1:5049511/68‑1 (MQ=255)                  aTACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGt                                                            <  1:3255532/56‑1 (MQ=255)                  aTACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCt                                             >  1:1650878/1‑71 (MQ=255)                     caccacGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAgg                                                         >  1:471017/1‑56 (MQ=255)                     cacaACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCa                                                                >  1:5395537/1‑49 (MQ=255)                     cacaACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGaa                                          >  1:2005261/1‑71 (MQ=255)                        aaCGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                       >  1:932886/1‑71 (MQ=255)                        aaCGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                       >  1:1961911/1‑71 (MQ=255)                        aaCGTTAGAAGTGATGGTCAGAACAGACTCAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                       <  1:1590691/71‑1 (MQ=255)                         aCGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                       >  1:5092046/1‑70 (MQ=255)                          cGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGGTTCTGAATTttc                                     <  1:4513366/71‑1 (MQ=255)                            ttAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                       <  1:1665726/67‑1 (MQ=255)                            ttAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                       <  1:478686/67‑1 (MQ=255)                            ttAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAATTttctt                                   <  1:3191103/71‑1 (MQ=255)                              aGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCa                                 >  1:4144598/1‑71 (MQ=255)                              aGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCa                                 >  1:1923857/1‑71 (MQ=255)                              aGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCa                                 >  1:2664794/1‑71 (MQ=255)                              aGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAATTTTCTTCa                                 >  1:3691980/1‑71 (MQ=255)                               gAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCAt                                <  1:3737269/71‑1 (MQ=255)                               gAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTGAATTTTCTTCAt                                <  1:903448/71‑1 (MQ=255)                                   tGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTttc                                     <  1:1842762/62‑1 (MQ=255)                                   tGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGa                              <  1:5430284/69‑1 (MQ=255)                                   tGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGa                              <  1:2555051/69‑1 (MQ=255)                                    gATGGTCAGAACAGACTGAGTCGGGATAGAGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc                           >  1:1607965/1‑71 (MQ=255)                                     aTGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAg                                                          >  1:1619658/1‑39 (MQ=255)                                     aTGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc                           <  1:5169601/70‑1 (MQ=255)                                     aTGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc                           <  1:2557183/70‑1 (MQ=255)                                     aTGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACg                          <  1:411506/71‑1 (MQ=255)                                     aTGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACg                          >  1:4177682/1‑71 (MQ=255)                                        gTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGGGTGCAGTTTCTGAATTTTCTTCATGAAACGtt                        <  1:470384/70‑1 (MQ=255)                                         tCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCt                      <  1:836843/71‑1 (MQ=255)                                          cAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                       >  1:3275993/1‑53 (MQ=255)                                          cAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                       >  1:512016/1‑53 (MQ=255)                                           aGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTa                     >  1:457559/1‑70 (MQ=255)                                            gAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTAcc                   <  1:4320318/71‑1 (MQ=255)                                             aaCAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc                           <  1:727378/62‑1 (MQ=255)                                             aaCAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc                           <  1:3001829/62‑1 (MQ=255)                                             aaCAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc                           <  1:4152402/62‑1 (MQ=255)                                              aCAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAg                                                  >  1:5564359/1‑38 (MQ=255)                                              aCAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTg                                            >  1:2980204/1‑44 (MQ=255)                                               cAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAgg                >  1:2523699/1‑71 (MQ=255)                                                  aCTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc                           <  1:1551903/57‑1 (MQ=255)                                                  aCTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCa             <  1:5237242/71‑1 (MQ=255)                                                  aCTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCa             <  1:712001/71‑1 (MQ=255)                                                  aCTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGATCTACCAGGTc              >  1:2196096/1‑70 (MQ=255)                                                   cTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                       <  1:4296603/44‑1 (MQ=255)                                                   cTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                       <  1:4267765/44‑1 (MQ=255)                                                    tGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                       <  1:1389460/43‑1 (MQ=255)                                                        tCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTc              <  1:2804514/64‑1 (MQ=255)                                                         cGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                       <  1:5526022/38‑1 (MQ=255)                                                         cGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGt                         <  1:1309733/52‑1 (MQ=255)                                                         cGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGt                         <  1:3884306/52‑1 (MQ=255)                                                          gggATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTGTACCAGGTCAACAGCCAg     >  1:3342243/1‑71 (MQ=255)                                                          gggATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAg     >  1:3315199/1‑71 (MQ=255)                                                          gggATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAg     >  1:4812152/1‑71 (MQ=255)                                                          gggATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAg     <  1:4270063/71‑1 (MQ=255)                                                          gggATAGCGTCAAGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAg     >  1:1855319/1‑71 (MQ=255)                                                             aTAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTc  <  1:5285724/71‑1 (MQ=255)                                                               aGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACg                          >  1:4113556/1‑45 (MQ=255)                                                               |                                                                     TTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTC  >  Exported/608398‑608528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑