Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 15,386 C→T 40.7% intergenic (+88/+59) dnaJ → / ← mokC chaperone Hsp40, co‑chaperone with DnaK/regulatory protein for HokC, overlaps CDS of hokC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported15,3860CT40.7% 51.2 / 92.4 89intergenic (+88/+59)dnaJ/mokCchaperone Hsp40, co‑chaperone with DnaK/regulatory protein for HokC, overlaps CDS of hokC
Reads supporting (aligned to +/- strand):  ref base C (33/18);  new base T (24/12);  total (59/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGTGCTCTCTT  >  Exported/15321‑15453
                                                                 |                                                                   
gtgtgctgtctgcgtcaatcgcagcactggtgctgtaagaccatacataaaaaagccggggataATTCCCa                                                                >  1:943614‑M1/65‑71 (MQ=255)
 tgtgctgtctgcgtcaatcgcagcactggtgctgtaagaccatacataaaaaagccggggataATTCCCa                                                                <  1:257710‑M1/7‑1 (MQ=255)
  cAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATa                                                              <  1:239958/71‑1 (MQ=255)
  cAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATa                                                              <  1:2048287/71‑1 (MQ=255)
    ggCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAg                                                            >  1:4577953/1‑71 (MQ=255)
    ggCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAg                                                            <  1:5268229/71‑1 (MQ=255)
         ctgcgtcaatcgcagcactggtgctgtaagaccatacataaaaaagccggggataATTCCCATAAGCGCt                                                        <  1:4305534‑M1/15‑1 (MQ=255)
          tgcgtcaatcgcagcactggtgctgtaagaccatacataaaaaagccggggataATTCCCATAAGCGCt                                                        >  1:4033767‑M1/55‑69 (MQ=255)
              aaaaTAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACtt                                                   <  1:670887/70‑1 (MQ=255)
              aaaaTAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACtt                                                   <  1:3810293/70‑1 (MQ=255)
                 atcgcaggactggtgctgtaagaccatacataaaaaagccggggataATTCCCATAAGCGCTa                                                       >  1:2688484‑M1/48‑63 (MQ=255)
                 aTAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTc                                                                   >  1:1511778/1‑51 (MQ=255)
                 aTAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAgg                                               >  1:5004610/1‑71 (MQ=255)
                 aTAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAgg                                               >  1:4962033/1‑71 (MQ=255)
                  tAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCa                                                                >  1:2943061/1‑53 (MQ=255)
                   cgcagcactggtgctgtaagaccatacataaaaaagccggggataATTCCCATAAGCGCTAACTTAAGGGt                                             <  1:3546389‑M1/26‑1 (MQ=255)
                     ggTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTtg                                           <  1:1359653/71‑1 (MQ=255)
                         cGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTgg                                        <  1:3912181/70‑1 (MQ=255)
                           ttGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCt                                                        <  1:2869625/52‑1 (MQ=255)
                           ttGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTa                                                       <  1:3843640/53‑1 (MQ=255)
                             gAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAg                                                            >  1:4078131/1‑46 (MQ=255)
                             gAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTaaa                                                 >  1:2503787/1‑57 (MQ=255)
                             gAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTgg                                        >  1:2031026/1‑66 (MQ=255)
                             gAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTgg                                        >  1:1918854/1‑66 (MQ=255)
                             gAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTgg                                        <  1:3366324/66‑1 (MQ=255)
                             gAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTAtt                                    >  1:2917311/1‑70 (MQ=255)
                             gAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTAtt                                    >  1:36268/1‑70 (MQ=255)
                             gAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTAtt                                    <  1:185245/70‑1 (MQ=255)
                             gAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTa                                   >  1:4165477/1‑71 (MQ=255)
                             gAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTa                                   >  1:3343144/1‑71 (MQ=255)
                             gAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTa                                   >  1:1749733/1‑71 (MQ=255)
                              tgctgtaagaccatacataaaaaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTAt                                     >  1:3267779‑M1/35‑68 (MQ=255)
                                  tatGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCt                              <  1:838172/71‑1 (MQ=255)
                                  gtaagaccatacataaaaaagccggggataATTCCCATAAGCGCt                                                        >  1:678103‑M1/31‑45 (MQ=255)
                                   taagaccatacataaaaaagccggggataATTCCCATAAGCGCTaa                                                      <  1:1919232‑M1/17‑1 (MQ=255)
                                   taagaccatacataaaaaagccggggataATTCCCATAAGCGCTAACTTaa                                                 >  1:121242‑M1/30‑51 (MQ=255)
                                   taagaccatacataaaaaagccggggataATTCCCATAAGCGCTAACTTaa                                                 >  1:3175185‑M1/30‑51 (MQ=255)
                                   atGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTaa                                                 >  1:3232604/1‑51 (MQ=255)
                                    tGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTg                             <  1:4680564/70‑1 (MQ=255)
                                    tGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGa                            >  1:4426033/1‑71 (MQ=255)
                                     agaccatacataaaaaagccggggataATTCCCATaa                                                             <  1:4792338‑M1/10‑1 (MQ=255)
                                     agaccatacataaaaaagccggggataATTCCCATaa                                                             <  1:943248‑M1/10‑1 (MQ=255)
                                     agaccatacataaaaaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGa                            <  1:3920470‑M1/43‑1 (MQ=255)
                                      gaccatacataaaaaagccggggataATTCCCATAAg                                                            >  1:1566550‑M1/27‑37 (MQ=255)
                                      gaccatacataaaaaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGata                          >  1:430829‑M1/27‑71 (MQ=255)
                                      gaccatacataaaaaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGata                          >  1:3729055‑M1/27‑71 (MQ=255)
                                      cGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGata                          >  1:2851192/1‑71 (MQ=255)
                                      cGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGata                          >  1:3893431/1‑71 (MQ=255)
                                      cGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGata                          >  1:3521764/1‑71 (MQ=255)
                                           tacataaaaaagccggggataATTCCCATAAGCGCTa                                                       >  1:2993137‑M1/22‑37 (MQ=255)
                                             cataaaaaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGAttt                    <  1:2832823‑M1/51‑1 (MQ=255)
                                             cataaaaaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGAttt                    <  1:1844446‑M1/51‑1 (MQ=255)
                                             cataaaaaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTa                   >  1:2844822‑M1/20‑71 (MQ=255)
                                             cataaaaaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTa                   >  1:2926393‑M1/20‑71 (MQ=255)
                                             cTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGAttt                    >  1:1797511/1‑70 (MQ=255)
                                             cTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGAttt                    >  1:3994974/1‑70 (MQ=255)
                                              ataaaaaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTAt                                     >  1:4492979‑M1/19‑52 (MQ=255)
                                               taaaaaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAc                 >  1:2924584‑M1/18‑71 (MQ=255)
                                               taaaaaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAc                 >  1:728401‑M1/18‑71 (MQ=255)
                                               gTAAAGTGGCGGGGATCACTCCCATAAGCGCTAAc                                                     >  1:1057744/1‑35 (MQ=255)
                                                tAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGt                                       <  1:5519796/48‑1 (MQ=255)
                                                 aaaaagccggggataATTCCCATAAGCGCTAACTTAAGGGTtgt                                          <  1:4050221‑M1/29‑1 (MQ=255)
                                                 aaaaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTAt                                     >  1:2929129‑M1/16‑49 (MQ=255)
                                                  aaGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGata                          >  1:3155987/1‑59 (MQ=255)
                                                  aaGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGa                       >  1:1153470/1‑62 (MQ=255)
                                                  aaGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGa                       >  1:2533086/1‑62 (MQ=255)
                                                  aaGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAcgtg              >  1:2637311/1‑71 (MQ=255)
                                                   aaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGata                          >  1:3117963‑M1/14‑58 (MQ=255)
                                                   aaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGata                          >  1:4279325‑M1/14‑58 (MQ=255)
                                                   aaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAtgtgc             >  1:2164116‑M1/14‑71 (MQ=255)
                                                    aagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTAtt                                    >  1:1553901‑M1/13‑47 (MQ=255)
                                                    aagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTAtt                                    >  1:2420322‑M1/13‑47 (MQ=255)
                                                    aagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAcgtgcc            >  1:3586730‑M1/13‑71 (MQ=255)
                                                    aagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAcgtgcc            >  1:2920698‑M1/13‑71 (MQ=255)
                                                     gtgCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAcgtgcc            >  1:1430147/3‑70 (MQ=25)
                                                      ggCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAcgtgccga          >  1:2049893/1‑70 (MQ=255)
                                                       ccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTaacgagccgat         <  1:4117568‑M1/62‑3 (MQ=255)
                                                        cggggagtAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTtaacgtgccgatg        >  1:4819287‑M1/9‑68 (MQ=255)
                                                        cGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAcgt               <  1:1552983/64‑1 (MQ=255)
                                                          gggATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTa                                      >  1:1749436/1‑39 (MQ=255)
                                                          gggATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTa                                      >  1:1784684/1‑39 (MQ=255)
                                                          gggATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGa                            <  1:4752532/49‑1 (MQ=255)
                                                             ataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGa                       <  1:3708574‑M1/48‑1 (MQ=255)
                                                             aTCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAcgtgc             <  1:1707647/61‑1 (MQ=255)
                                                              tCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGTGCTCtctt  <  1:771877/71‑1 (MQ=255)
                                                               cACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAcgtgccg           >  1:1952393/1‑61 (MQ=255)
                                                               cACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAcgtgccg           >  1:1623064/1‑61 (MQ=255)
                                                               cACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAcgtgccg           >  1:4524062/1‑61 (MQ=255)
                                                               cACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAcgtgccg           >  1:4516927/1‑61 (MQ=255)
                                                                 cTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGAttt                    <  1:2570064/50‑1 (MQ=255)
                                                                 cTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAcgtgccga          >  1:1659374/1‑59 (MQ=255)
                                                                 cTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAcgtgccga          >  1:2036171/1‑59 (MQ=255)
                                                                 |                                                                   
GGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGTGCTCTCTT  >  Exported/15321‑15453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: