BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	608,901	A→C	13.9%	V451V (GTT→GTG)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	608,901	0	A	C	13.9%	224.4 / 36.5	108	V451V (GTT→GTG)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base A (50/43); new base C (7/8); total (57/51)
Fisher's exact test for biased strand distribution p-value = 7.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 121 total aligned reads displayed.

ATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTT  >  Exported/608832‑608971

atcatAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCaa                                                                       >  1:5548118/1‑71 (MQ=255)

atcatAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCaa                                                                       >  1:1828819/1‑71 (MQ=255)

atcatAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCa                                                                        <  1:3371711/70‑1 (MQ=255)

atcatAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCa                                                                        <  1:2193148/70‑1 (MQ=255)

atcatAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCa                                                                        <  1:4369909/70‑1 (MQ=255)

atcatAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCa                                                                        <  1:1591369/70‑1 (MQ=255)

atcatAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCa                                                                        <  1:4223156/70‑1 (MQ=255)

atcatAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCa                                                                        <  1:3095531/70‑1 (MQ=255)

 tcatAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAAc                                                                      <  1:1910813/71‑1 (MQ=255)

 tcatAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAAc                                                                      <  1:706396/71‑1 (MQ=255)

   atAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAAcgc                                                                    >  1:4099931/1‑71 (MQ=255)

          aGGATATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGtt                                                             >  1:4215052/1‑71 (MQ=255)

          aGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGtt                                                             >  1:808107/1‑71 (MQ=255)

          aGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGtt                                                             >  1:4941894/1‑71 (MQ=255)

          aGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGtt                                                             <  1:2648773/71‑1 (MQ=255)

           ggACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTg                                                            <  1:3891587/71‑1 (MQ=255)

           ggACATAGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGtt                                                             >  1:2870897/1‑70 (MQ=255)

             aCATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGAt                                                          >  1:1881430/1‑71 (MQ=255)

               aTCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGa                                                           <  1:2356034/68‑1 (MQ=255)

                tCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGc                                                       <  1:2574888/71‑1 (MQ=255)

                tCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGc                                                       <  1:4092283/71‑1 (MQ=255)

                 cGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCg                                                      >  1:3047628/1‑71 (MQ=255)

                 cGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCg                                                      >  1:2707379/1‑71 (MQ=255)

                  gCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCaa                                                                       >  1:1206350/1‑53 (MQ=255)

                   ccTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGAt                                                          >  1:4518497/1‑65 (MQ=255)

                   ccTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTa                                                    <  1:4510550/71‑1 (MQ=255)

                   ccTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTa                                                    <  1:4843710/71‑1 (MQ=255)

                   ccTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTa                                                    <  1:2868979/71‑1 (MQ=255)

                     tttGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTa                                                    >  1:1517222/1‑69 (MQ=255)

                        gATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGa                                                           >  1:1843127/1‑59 (MQ=255)

                        gATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGa                                                           >  1:2231155/1‑59 (MQ=255)

                            ggTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAAAGCCGCCGTTGATTGCGTACAGCATGGt                                           <  1:3284944/71‑1 (MQ=255)

                             gTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGtt                                          <  1:65963/71‑1 (MQ=255)

                              ttCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGttt                                         <  1:4326348/71‑1 (MQ=255)

                              ttCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGttt                                         <  1:5431602/71‑1 (MQ=255)

                                cAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGtttt                                        >  1:5268579/1‑70 (MQ=255)

                                cAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTc                                       >  1:4130640/1‑71 (MQ=255)

                                  gACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGc                                     >  1:1264034/1‑71 (MQ=255)

                                   aCTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGcc                                    <  1:1821597/71‑1 (MQ=255)

                                     ttCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGa                                                           <  1:4029474/46‑1 (MQ=255)

                                     ttCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCa                                               <  1:3575453/58‑1 (MQ=255)

                                     ttCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCa                                               <  1:1190732/58‑1 (MQ=255)

                                     ttCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCa                                               <  1:4165177/58‑1 (MQ=255)

                                     ttCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCa                                               <  1:2527513/58‑1 (MQ=255)

                                     ttCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATg                                             <  1:1342268/60‑1 (MQ=255)

                                     ttCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATg                                             <  1:4931906/60‑1 (MQ=255)

                                       cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATg                                             >  1:4370299/1‑58 (MQ=255)

                                       cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATg                                             >  1:3448639/1‑58 (MQ=255)

                                       cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGc                                     >  1:2451882/1‑66 (MQ=255)

                                       cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAgg                                 <  1:1832260/70‑1 (MQ=255)

                                       cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAgg                                 <  1:3010404/70‑1 (MQ=255)

                                       cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAg                                  <  1:808652/69‑1 (MQ=255)

                                       cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGt                                >  1:4920491/1‑71 (MQ=255)

                                       cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGt                                >  1:3961435/1‑71 (MQ=255)

                                            cgaCCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGcg                           >  1:5607572/3‑70 (MQ=255)

                                            cAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCa                           >  1:2086234/1‑71 (MQ=255)

                                             gaCCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGcgc                          >  1:4024147/2‑69 (MQ=255)

                                              aCCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGcc                                    >  1:2503781/1‑60 (MQ=255)

                                              aCCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAcg                         <  1:2942887/71‑1 (MQ=255)

                                              aCCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGcgcg                         <  1:62444/71‑1 (MQ=255)

                                                cTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAcgcg                       >  1:2418760/1‑71 (MQ=255)

                                                cTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAcgcg                       <  1:3899536/71‑1 (MQ=255)

                                                cTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGcgcgtg                       <  1:604767/71‑3 (MQ=255)

                                                  gCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTa                                                    >  1:2643916/1‑40 (MQ=255)

                                                  gCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTa                                                    >  1:2273886/1‑40 (MQ=255)

                                                  gCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCa                     >  1:2833316/1‑71 (MQ=255)

                                                   cATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCAc                    >  1:664257/1‑71 (MQ=255)

                                                     ttttCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCAcc                   >  1:3640368/1‑70 (MQ=255)

                                                     ttttCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCAcc                   >  1:3300909/1‑70 (MQ=255)

                                                       ttCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCg                  >  1:55847/1‑69 (MQ=255)

                                                        tCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCg                  >  1:4919861/1‑68 (MQ=255)

                                                         cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGcgcgtg                       >  1:5664726/1‑60 (MQ=255)

                                                         cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaag               <  1:3515728/70‑1 (MQ=255)

                                                         cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaag               <  1:5081114/70‑1 (MQ=255)

                                                         cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaag               <  1:3217052/70‑1 (MQ=255)

                                                          aGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa             <  1:2485728/71‑1 (MQ=255)

                                                            tttttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACt           <  1:3820837/71‑1 (MQ=255)

                                                             ttttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGtttt                                        >  1:899933/1‑41 (MQ=255)

                                                             ttttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGtttt                                        >  1:1781154/1‑41 (MQ=255)

                                                             ttttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTg          >  1:4708645/1‑71 (MQ=255)

                                                             ttctCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTg          >  1:5586913/4‑71 (MQ=255)

                                                              tttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAg                                  >  1:2557281/1‑46 (MQ=255)

                                                              tttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGtt                               >  1:3815029/1‑49 (MQ=255)

                                                              tttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGtt                               >  1:2630020/1‑49 (MQ=255)

                                                              tttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTg          >  1:2813492/1‑70 (MQ=255)

                                                              tttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTg          >  1:3031028/1‑70 (MQ=255)

                                                              tttCGTCAACGCCACCGTTGATTGCGTACAGCATGGTTTTCGCCAGGtt                               >  1:1673985/1‑49 (MQ=255)

                                                               ctCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGc         <  1:5301396/69‑1 (MQ=255)

                                                               ctCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGc         <  1:4795152/69‑1 (MQ=255)

                                                                tCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAt       >  1:1163239/1‑71 (MQ=255)

                                                                tCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAt       >  1:2057723/1‑71 (MQ=255)

                                                                tCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa             >  1:4202879/1‑65 (MQ=255)

                                                                tCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa             >  1:4648764/1‑65 (MQ=255)

                                                                tCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCa        <  1:2517823/70‑1 (MQ=255)

                                                                 cGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAtt      >  1:462022/1‑71 (MQ=255)

                                                                 cGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCAt       <  1:3273245/70‑1 (MQ=255)

                                                                   tCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTg    <  1:5268998/71‑1 (MQ=255)

                                                                   tCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTg    <  1:1694310/71‑1 (MQ=255)

                                                                     aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGtt  >  1:2402722/1‑71 (MQ=255)

                                                                     aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGtt  <  1:898099/71‑1 (MQ=255)

ATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTT  >  Exported/608832‑608971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`