Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	609,094	A→T	5.2%	F387Y (TTC→TAC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	609,094	0	A	T	5.2%	222.3 / 3.2	76	F387Y (TTC→TAC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base A (31/41);  new base T (2/2);  total (33/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

CCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCAT  >  Exported/609029‑609164                                                                  |                                                                       ccGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAAt                                                                      <  1:1150458/68‑1 (MQ=255)  cGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTc                                                                   <  1:5337889/70‑1 (MQ=255)  cGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTc                                                                   <  1:2198931/70‑1 (MQ=255)    gACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTg                                                                <  1:5231204/71‑1 (MQ=255)    gACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTg                                                                <  1:3077275/71‑1 (MQ=255)    gACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGAAACTTTAGCGGCGAATTTCtt                                                                 >  1:249047/1‑70 (MQ=255)     aCGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGa                                                               <  1:3909367/71‑1 (MQ=255)      cGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTg                                                                >  1:1036772/1‑69 (MQ=255)      cGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTg                                                                >  1:5175063/1‑69 (MQ=255)      cGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGa                                                               <  1:1731496/70‑1 (MQ=255)        cATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAgg                                                            <  1:1224327/71‑2 (MQ=255)         aTCAGATCGTCGTTTTCATACTGCAAAGAGGATGTGACGATAGACACCTGCGCGGCATATTTTTTGaa                                                              >  1:3717520/1‑68 (MQ=255)          tCAGTTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTc                                                          <  1:3847743/71‑1 (MQ=255)          tCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTg                                                                <  1:2152486/65‑1 (MQ=255)          tCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTc                                                          <  1:4503201/71‑1 (MQ=255)          tCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTc                                                          <  1:2073074/71‑1 (MQ=255)          tCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTc                                                          <  1:4737692/71‑1 (MQ=255)           cAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGtt                                                           >  1:4818782/1‑69 (MQ=255)            aGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGaa                                                              >  1:2111126/1‑65 (MQ=255)            aGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGaa                                                              >  1:1385118/1‑65 (MQ=255)            aGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGa                                                               >  1:2873580/1‑64 (MQ=255)               tCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCgg                                                     <  1:4179864/71‑1 (MQ=255)                cATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCgg                                                     >  1:5694392/1‑70 (MQ=255)                 aTCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGc                                                    <  1:5530304/70‑1 (MQ=255)                  tCGTTCTCATTCTGCAGAGAAGAGGTGTCGATGGACACTTTAGGGGCGAATttgttg                                                                <  1:3695301/57‑1 (MQ=255)                  tCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAg                                                  <  1:1416829/71‑1 (MQ=255)                    gTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGGGGCAGtt                                                >  1:145938/1‑71 (MQ=255)                    gTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGtt                                                >  1:3218500/1‑71 (MQ=255)                     ttCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGttt                                               <  1:5655573/71‑1 (MQ=255)                     ttCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGttt                                               <  1:701365/71‑1 (MQ=255)                       ctcATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGaa                                                                       >  1:2721395/1‑45 (MQ=255)                       ctcATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGaa                                                                       >  1:3134255/1‑45 (MQ=255)                       ctcATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGtttt                                              >  1:1640732/1‑70 (MQ=255)                       ctcATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGtttt                                              <  1:5499503/70‑1 (MQ=255)                       ctcATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGtttt                                              <  1:986105/70‑1 (MQ=255)                        tcATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTg                                                                <  1:2005458/51‑1 (MQ=255)                        tcATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTc                                                          <  1:2449680/57‑1 (MQ=255)                         cATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGttttt                                             >  1:3973005/1‑69 (MQ=255)                         cATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAAATTCTTGAAGTTCAGCGGCAGttttt                                             >  1:3973945/1‑69 (MQ=255)                          aTACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGttttt                                             <  1:2829398/68‑1 (MQ=255)                          aTACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGttttt                                             <  1:4208587/68‑1 (MQ=255)                                cAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGt                                                            >  1:3659735/1‑47 (MQ=255)                                cAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACca                                      >  1:4997603/1‑69 (MQ=255)                                 agagAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAg                                   <  1:2205120/71‑1 (MQ=255)                                  gagaAGAGGTGTCGATGGGCACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGa                                  <  1:2248125/71‑1 (MQ=255)                                  gagaAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCa                                                   >  1:3008616/1‑54 (MQ=255)                                  gagaAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCa                                                   >  1:439162/1‑54 (MQ=255)                                   agaagaGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAg                                   <  1:1388042/69‑1 (MQ=255)                                      agagGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATgg                              <  1:143769/71‑1 (MQ=255)                                          gtgtCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGt                             <  1:944340/68‑1 (MQ=255)                                           tgtCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCgg                                                     <  1:5172726/43‑1 (MQ=255)                                            gtCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGt                        >  1:5575644/1‑71 (MQ=255)                                             tCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGtt                       >  1:3521916/1‑71 (MQ=255)                                             tCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGt                        <  1:2777022/70‑1 (MQ=255)                                              cGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGaa                                 >  1:5057120/1‑60 (MQ=255)                                               gATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAg                                                        >  1:4742184/1‑36 (MQ=255)                                               gATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATg                         <  1:4558037/67‑1 (MQ=255)                                               gATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATg                         <  1:3906427/67‑1 (MQ=255)                                               gATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATg                         <  1:4903770/67‑1 (MQ=255)                                               gATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCg                     >  1:1249222/1‑71 (MQ=255)                                                 tGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACcac                                     <  1:4296934/53‑1 (MQ=255)                                                  ggACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGtt                  >  1:908052/1‑71 (MQ=255)                                                   gACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCg                     >  1:993016/1‑67 (MQ=255)                                                   gACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTc                 >  1:1119123/1‑71 (MQ=255)                                                    acacTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGttt                                               <  1:842993/40‑1 (MQ=255)                                                     cacTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCg               <  1:3235862/71‑1 (MQ=255)                                                      acTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCgg              <  1:5056921/71‑1 (MQ=255)                                                      acTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCgg              <  1:4839738/71‑1 (MQ=255)                                                       ctatAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCgg                    <  1:1968538/61‑1 (MQ=255)                                                       cTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGtt                  >  1:196748/1‑66 (MQ=255)                                                        tttAGCGGCGAATTTCTTGAAGTTCAGCGGCAGtttt                                              <  1:65577/37‑1 (MQ=255)                                                         ttAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGAc                                        >  1:4845067/1‑42 (MQ=255)                                                         ttAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGAc                                        >  1:687612/1‑42 (MQ=255)                                                         ttAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTc                      >  1:3949843/1‑60 (MQ=255)                                                         ttAGCGGCGAATTTCTTGAAGTACAGCGGCAGTTTTTCAGAc                                        >  1:3069775/1‑42 (MQ=255)                                                          tAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGAc          >  1:2382759/1‑71 (MQ=255)                                                               gcgAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGga             <  1:1774134/63‑1 (MQ=255)                                                                 gAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCa   >  1:3962840/1‑71 (MQ=255)                                                                  aaTTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAAt                                <  1:5091665/41‑1 (MQ=255)                                                                  aaTTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGtt                  <  1:4771914/55‑1 (MQ=255)                                                                  aaTTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCAt  <  1:5113698/71‑1 (MQ=255)                                                                  |                                                                       CCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCAT  >  Exported/609029‑609164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑