Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	609,242	A→C	9.1%	S338A (TCT→GCT) ‡	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	609,242	0	A	C	9.1%	180.9 / 11.9	77	S338A (TCT→GCT) ‡	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base A (36/34);  new base C (2/5);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

GGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCA  >  Exported/609173‑609310                                                                      |                                                                     gggTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATAACACCGATAGAt                                                                     <  1:4219930/71‑1 (MQ=255)      ttCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGt                                                                >  1:146097/1‑71 (MQ=255)         aGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTg                                                              <  1:1815458/70‑1 (MQ=255)         aGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGc                                                             <  1:3223435/71‑1 (MQ=255)             aaCGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAg                                                         <  1:5120824/71‑1 (MQ=255)              aCGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGa                                                        <  1:1618778/71‑1 (MQ=255)               cGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGcc                                                            >  1:2858751/1‑66 (MQ=255)               cGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGAt                                                       <  1:1468218/71‑1 (MQ=255)                ggAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGcc                                                            >  1:4980736/1‑65 (MQ=255)                   aGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCgg                                                    <  1:2476638/70‑1 (MQ=255)                    ggagTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGt                                                  <  1:2964678/68‑1 (MQ=255)                       gTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCgg                                                                 >  1:4654558/1‑53 (MQ=255)                        tttttGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTggg                                                   >  1:4588029/1‑66 (MQ=255)                        tttttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGc                                               <  1:151642/70‑1 (MQ=255)                         ttttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGc                                               >  1:1515688/1‑69 (MQ=255)                         ttttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGc                                               >  1:2138134/1‑69 (MQ=255)                          tttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCg                                                     >  1:2000489/1‑62 (MQ=255)                          tttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGcc                                              >  1:2712682/1‑69 (MQ=255)                          tttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGcc                                              >  1:2474570/1‑69 (MQ=255)                           ttGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGccgg                                            <  1:1269378/70‑3 (MQ=255)                             ggTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCagag                                          >  1:1969865/1‑70 (MQ=255)                              gtcaCCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGaa                                        <  1:4104932/68‑1 (MQ=255)                              gTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAgaga                                         <  1:2512797/70‑1 (MQ=255)                                 aCCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAg                                     >  1:845085/1‑71 (MQ=255)                                 aCCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGcc                                              <  1:765651/62‑1 (MQ=255)                                 aCCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAg                                     <  1:3508966/71‑1 (MQ=255)                                   cAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGtt                                   >  1:4221212/1‑71 (MQ=255)                                   cAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGt                                    >  1:2647042/1‑70 (MQ=255)                                   cAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGt                                    >  1:3660695/1‑70 (MQ=255)                                   cAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGccgg                                            >  1:2796722/1‑60 (MQ=255)                                      ggTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCAt                                <  1:1293650/71‑1 (MQ=255)                                        tACGACCGTCGAGGCCCATACCACCGATAGATTCgg                                                                 >  1:4678409/1‑36 (MQ=255)                                        tACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCg                              <  1:2079290/71‑1 (MQ=255)                                        tACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCg                              <  1:3889893/71‑1 (MQ=255)                                          cGACCGTCGAGGCCCATACCACCGATAGATTCGGTTg                                                              <  1:3607041/37‑1 (MQ=255)                                          cGACCGTCGAGGCCCATACCACCGATAGATTCGGTTg                                                              <  1:3613668/37‑1 (MQ=255)                                            aCCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTAtt                          >  1:1698968/1‑71 (MQ=255)                                            aCCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTAt                           >  1:624818/1‑70 (MQ=255)                                             ccGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTc                         >  1:572776/1‑71 (MQ=255)                                             ccGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTc                         >  1:4124624/1‑71 (MQ=255)                                               gTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCgg                                                    <  1:2518763/42‑1 (MQ=255)                                               gTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCg                       <  1:850734/71‑1 (MQ=255)                                               gTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCg                       <  1:1691527/71‑1 (MQ=255)                                                tCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACa                                      <  1:3899602/55‑1 (MQ=255)                                                tCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACa                                      <  1:3307660/55‑1 (MQ=255)                                                tCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACa                                      <  1:4986670/55‑1 (MQ=255)                                                tCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACa                                      <  1:4247526/55‑1 (MQ=255)                                                tCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCg                       >  1:4205076/1‑70 (MQ=255)                                                   aGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAg                    >  1:4514107/1‑70 (MQ=255)                                                       ccATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTGCGGAGTACg                >  1:3484677/1‑70 (MQ=255)                                                        cATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAAc                                       <  1:2868013/46‑1 (MQ=255)                                                        cATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAAc                                       <  1:3358088/46‑1 (MQ=255)                                                        cATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAAc                                       <  1:4291754/46‑1 (MQ=255)                                                         aTACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAAc                                       <  1:4092524/45‑1 (MQ=255)                                                         aTACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAg             >  1:4629968/1‑71 (MQ=255)                                                          tACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAAc                                       >  1:2804630/1‑44 (MQ=255)                                                          tACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAg                                     >  1:797551/1‑46 (MQ=255)                                                          tACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAgg            <  1:1712763/71‑1 (MQ=255)                                                          tACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAgg            <  1:3820930/71‑1 (MQ=255)                                                            ccaccGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGa           <  1:821195/70‑1 (MQ=255)                                                            ccaccGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGa           <  1:3361070/70‑1 (MQ=255)                                                              accGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCg                       >  1:378396/1‑56 (MQ=255)                                                               ccGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTc                         >  1:5616054/1‑53 (MQ=255)                                                                cGATAGATTCGGTTGCCCAGATCGGGTCGCCAgaga                                         >  1:2719212/1‑36 (MQ=255)                                                                cGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGaa                                        >  1:4196245/1‑37 (MQ=255)                                                                cGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCg       <  1:1657633/70‑1 (MQ=255)                                                                cGATAGATTCGGTTGCCCAGATCGGGTAGCCAGAGaa                                        >  1:2396641/1‑37 (MQ=255)                                                                 gATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATc                               >  1:503729/1‑45 (MQ=255)                                                                  aTAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTc                                  <  1:5490046/41‑1 (MQ=255)                                                                  aTAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAc    >  1:4952182/1‑71 (MQ=255)                                                                   tAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTc                         <  1:4114301/49‑1 (MQ=255)                                                                   tAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGa      <  1:2988351/68‑1 (MQ=255)                                                                    aGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGc        >  1:1460356/1‑65 (MQ=255)                                                                    aGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAcc   >  1:5282517/1‑70 (MQ=255)                                                                    aGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAc    >  1:4756260/1‑69 (MQ=255)                                                                    aGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCa  >  1:399609/1‑71 (MQ=255)                                                                     gATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCa  <  1:4702818/70‑1 (MQ=255)                                                                      |                                                                     GGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCA  >  Exported/609173‑609310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑