| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 189,490 | C→T | 10.8% | F329F (TTC→TTT) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 189,490 | 0 | C | T | 10.8% | 194.8 / 10.9 | 74 | F329F (TTC→TTT) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (32/33); new base T (5/3); total (38/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
CCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGG > Exported/189420‑189555 | ccACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTc > 1:4535807/1‑71 (MQ=255) aCCTATGATGTCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGa < 1:1400329/71‑1 (MQ=255) aCCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGa < 1:3830497/71‑1 (MQ=255) aCCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGa < 1:4100464/71‑1 (MQ=255) cTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCg < 1:2277606/68‑1 (MQ=255) atgatgGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGAt > 1:4954634/1‑68 (MQ=255) tgatgGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTc < 1:4663397/64‑1 (MQ=255) tgatgGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTg > 1:5642568/1‑71 (MQ=255) atgGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGcc > 1:1373105/1‑71 (MQ=255) tgGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCt < 1:4418760/71‑1 (MQ=255) gCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTg < 1:614753/70‑1 (MQ=255) ttGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGt > 1:2674363/1‑71 (MQ=255) ttGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGt > 1:2428289/1‑71 (MQ=255) tGCTGTACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTg > 1:636499/1‑71 (MQ=255) tGCTGTACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTg > 1:69485/1‑71 (MQ=255) tGCTGTACAACACCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTg > 1:130997/1‑71 (MQ=255) gCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGc > 1:2076418/1‑71 (MQ=255) ctctACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGcc > 1:2328671/1‑71 (MQ=255) ctctACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGcc < 1:5310417/71‑1 (MQ=255) ctACAACACCGACTGGATCAAACAGCCGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCg > 1:2405386/1‑70 (MQ=255) caacaCCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGCCTTaca > 1:2258878/1‑71 (MQ=255) caacaCCGACTTCATCAAGAAAACACTGGATGTGAAATCCATCCACTTTGACTCCGCGTGGGTGCCTTaca > 1:19933/1‑71 (MQ=255) caacaCCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTaca > 1:485429/1‑71 (MQ=255) aacaCCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTacac > 1:2438796/1‑71 (MQ=255) acacCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGt < 1:5215632/61‑1 (MQ=255) acacCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGt < 1:4547776/61‑1 (MQ=255) acacCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGt < 1:3466722/61‑1 (MQ=255) acacCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGt < 1:302618/61‑1 (MQ=255) cacCGACTGGACCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACAccc < 1:5215005/71‑1 (MQ=255) acCGTCTGGATCAAAGAGACGCTGGATGTCCCGTCGATTGACTTCGATTGTGCCTGGGTGCCGTACACCCa < 1:5465383/71‑1 (MQ=255) ccGACTGGAACAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGc > 1:770251/1‑52 (MQ=255) cGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTAGATTCTGCCTGGGTGCCGTACACCCA‑tt > 1:7307/1‑71 (MQ=255) aCTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGAt > 1:853719/1‑44 (MQ=255) aCTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGAt > 1:4881135/1‑44 (MQ=255) aCTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGAt > 1:1169679/1‑44 (MQ=255) aCTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGAt > 1:591502/1‑44 (MQ=255) aCTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGAt > 1:1960778/1‑44 (MQ=255) aCTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGAt > 1:2722826/1‑44 (MQ=255) aCTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGAt > 1:3646242/1‑44 (MQ=255) tGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCg < 1:1010732/40‑1 (MQ=255) tGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCg < 1:3705390/40‑1 (MQ=255) cAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGa > 1:3387964/1‑71 (MQ=255) aaaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTgg > 1:2431789/1‑45 (MQ=255) aaaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTgg > 1:2494008/1‑45 (MQ=255) aaaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTgg > 1:801927/1‑45 (MQ=255) aaaCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTgg > 1:4588438/1‑45 (MQ=255) aCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTacac > 1:2049515/1‑54 (MQ=255) aCAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTaca > 1:4164848/1‑53 (MQ=255) cAGACGCTGTATGTCCCGTCGATTCACTTCTATTCTg < 1:5132272/37‑1 (MQ=38) cAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTa > 1:1274597/1‑71 (MQ=255) cAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTa < 1:3199739/71‑1 (MQ=255) aCGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGAt > 1:4213022/1‑65 (MQ=255) cGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCa < 1:1872445/70‑1 (MQ=255) cGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAg > 1:317984/1‑71 (MQ=255) gATGTCCCGTCGATTCACTTTGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTaa < 1:5687877/71‑1 (MQ=255) gATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCTATCTACCAGGGTaa < 1:5057101/71‑1 (MQ=255) gATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCTATCTACCAGGGTaa < 1:3289155/71‑1 (MQ=255) gATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTaa < 1:4871286/71‑1 (MQ=255) tGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTaaaa < 1:862260/71‑1 (MQ=255) tCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAg < 1:1131259/70‑1 (MQ=255) cGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCa < 1:684431/58‑1 (MQ=255) cGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCa < 1:4283153/58‑1 (MQ=255) cGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCa < 1:4289751/58‑1 (MQ=255) tCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTAcc < 1:359900/55‑1 (MQ=255) tCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTAcc < 1:1856686/55‑1 (MQ=255) tCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTAcc < 1:4846197/55‑1 (MQ=255) gATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTGGTa > 1:4615643/1‑68 (MQ=255) gATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTGGTAt > 1:792084/1‑69 (MQ=255) gATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTGGTAt > 1:2351111/1‑69 (MQ=255) gATTCACTTCGATTCTGCCTAGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTGGTATGa < 1:4226539/71‑1 (MQ=255) tCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAgcg > 1:2957342/1‑71 (MQ=255) cACTTTGACTCCGCGTTGGTGCCTTACACCAACTTCTCA‑CCGATTTACGAAGGTAAATGCGGTATGAgcg < 1:2040140/70‑1 (MQ=255) cACTTTGACTCCGCGTGGGTGCCTTACACCAACTTCTCA‑CCGATTTACGAAGGTAAATGCGGTATGAgcgg < 1:609115/71‑1 (MQ=255) aCTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTaa < 1:1966890/55‑1 (MQ=255) aCTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTaa < 1:5018399/55‑1 (MQ=255) cTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTAc < 1:3561015/46‑1 (MQ=255) cGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGAt > 1:3369274/1‑39 (MQ=255) cGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGAt > 1:2358286/1‑39 (MQ=255) cGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCa < 1:2805639/45‑1 (MQ=255) cGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAgg > 1:4457879/1‑47 (MQ=255) cGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAgg > 1:5009936/1‑47 (MQ=255) cGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAg > 1:4993203/1‑46 (MQ=255) | CCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTGGGTGCCGTACACCCA‑TTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGG > Exported/189420‑189555 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |