BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	609,708	G→A	16.0%	Y182Y (TAC→TAT)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	609,708	0	G	A	16.0%	259.2 / 33.5	106	Y182Y (TAC→TAT)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base G (50/39); new base A (8/9); total (58/48)
Fisher's exact test for biased strand distribution p-value = 5.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

Only 100 of 109 total aligned reads displayed.

CTGCAGAGAAGTGAACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGC  >  Exported/609639‑609775

cTGCAGAGAAGTGAACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGt                                                                    <  1:927197/71‑1 (MQ=255)

       gaAGTGAACTTTTCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCAcc                                                             <  1:161517/71‑1 (MQ=255)

        aaGTGAACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACgg                                                                     <  1:757716/62‑1 (MQ=255)

         aGTGAACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCg                                                            <  1:5489663/70‑1 (MQ=255)

            gAACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACagtagtaa                                                               >  1:4271934/1‑62 (MQ=255)

            gAACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCga                                                           >  1:1597522/1‑68 (MQ=255)

             aaCTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAAGGCGACGGTAGTCACCgat                                                          >  1:4388833/1‑68 (MQ=255)

             aaCTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatga                                                        >  1:5592300/1‑70 (MQ=255)

              aCTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatga                                                        <  1:4533258/69‑1 (MQ=255)

               cTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatga                                                        <  1:1501583/68‑1 (MQ=255)

                tgtgCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATacg                                                    >  1:227242/1‑71 (MQ=255)

                  tgCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatga                                                        >  1:5575671/1‑65 (MQ=255)

                  tgCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATacggc                                                  >  1:274207/1‑71 (MQ=255)

                  tgCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATacggc                                                  >  1:58150/1‑71 (MQ=255)

                     cAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCAcc                                                             <  1:1285523/57‑1 (MQ=255)

                     cAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATacggccac                                               <  1:3335765/71‑1 (MQ=255)

                     cAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATacggccac                                               >  1:4086692/1‑71 (MQ=255)

                         ttGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATTATacggccacggc                                            <  1:939193/70‑1 (MQ=255)

                          tGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCGCCgatgat                                                       >  1:1554669/1‑58 (MQ=255)

                          tGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatgat                                                       >  1:5210927/1‑58 (MQ=255)

                          tGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatgat                                                       >  1:1510659/1‑58 (MQ=255)

                           gTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTa                                                                   >  1:2004540/1‑45 (MQ=255)

                           gTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATAcggccacggcca                                          <  1:4981263/70‑1 (MQ=255)

                           gTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATAcggccacggcca                                          <  1:4987662/70‑1 (MQ=255)

                           gTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATAcggccacggcca                                          >  1:4368058/1‑70 (MQ=255)

                             cTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatg                                                         >  1:4441436/1‑53 (MQ=255)

                             cTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatg                                                         >  1:1698368/1‑53 (MQ=255)

                             cTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatg                                                         >  1:1497996/1‑53 (MQ=255)

                             cTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatg                                                         >  1:1216460/1‑53 (MQ=255)

                               ttCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCata                                        >  1:572454/1‑68 (MQ=255)

                                tcatcaGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGc                                     <  1:2795615/70‑1 (MQ=255)

                                tcatcaGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCa                                    >  1:5208177/1‑71 (MQ=255)

                                  atcaGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCAt                                   <  1:739018/70‑1 (MQ=255)

                                   tcaGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCt                                 <  1:5165410/71‑1 (MQ=255)

                                   tcaGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCt                                 <  1:2921582/71‑1 (MQ=255)

                                       gTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATAcggccacggcca                                          <  1:3335217/58‑1 (MQ=255)

                                          gTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGAc                          <  1:1626945/71‑1 (MQ=255)

                                           tCGATACCGTACAGCGCACCGCGACGGTAGTCAcc                                                             <  1:2471051/35‑1 (MQ=255)

                                           tCGATACCGTACAGCGCAACGCGACGGTAGTCAcc                                                             <  1:2487276/35‑1 (MQ=255)

                                           tCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGAc                          <  1:5438625/70‑1 (MQ=255)

                                            tgATGCCATACAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCg                        >  1:4832129/2‑71 (MQ=255)

                                            cGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCg                        >  1:3647090/1‑71 (MQ=255)

                                             gATACCTTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCgg                       >  1:2364034/1‑71 (MQ=255)

                                             gATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATAcggccacggcca                                          <  1:4626382/52‑1 (MQ=255)

                                             gATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATAcggccacggcca                                          <  1:2112933/52‑1 (MQ=255)

                                             gATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCgg                       >  1:4304771/1‑71 (MQ=255)

                                               tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATacggccacggc                                            >  1:3762709/1‑48 (MQ=255)

                                               tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCAt                                   >  1:5246443/1‑57 (MQ=255)

                                               tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCAt                                   >  1:3107127/1‑57 (MQ=255)

                                               tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCAt                                   >  1:867204/1‑57 (MQ=255)

                                               tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCAt                                   >  1:2625745/1‑57 (MQ=255)

                                               tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCAt                                   >  1:2385760/1‑57 (MQ=255)

                                               tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCAt                                   >  1:5135949/1‑57 (MQ=255)

                                               tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGt                      >  1:1882342/1‑70 (MQ=255)

                                               tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGt                      >  1:652340/1‑70 (MQ=255)

                                               tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGACACGGCCATATGCAt                                   >  1:5125436/1‑57 (MQ=255)

                                                 ccatACAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATGTGGTAAACCGGTCAg                   <  1:3584754/68‑1 (MQ=255)

                                                 ccatACAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAg                   <  1:1652425/68‑1 (MQ=255)

                                                 ccGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCata                                        <  1:3301076/50‑1 (MQ=255)

                                                 ccGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCata                                        <  1:1825870/50‑1 (MQ=255)

                                                 ccGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAg                   <  1:819147/71‑1 (MQ=255)

                                                 ccGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAg                   <  1:4061678/71‑1 (MQ=255)

                                                 ccGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAg                   <  1:4882749/71‑1 (MQ=255)

                                                   gTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGaa                 >  1:1245798/1‑71 (MQ=255)

                                                    tACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCa                                    <  1:4261029/51‑1 (MQ=255)

                                                    tACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGaa                 >  1:1790933/1‑70 (MQ=255)

                                                     aCAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCa                                    <  1:3353916/50‑1 (MQ=255)

                                                      cAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggt                              >  1:2969872/1‑54 (MQ=16)

                                                      cAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggt                              >  1:1897407/1‑54 (MQ=16)

                                                      cAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggt                              >  1:2959397/1‑54 (MQ=16)

                                                      cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCAt                                   >  1:2296876/1‑50 (MQ=255)

                                                      cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATc                                  >  1:2302745/1‑51 (MQ=255)

                                                      cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATc                                  >  1:3114443/1‑51 (MQ=255)

                                                      cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAacac              >  1:3290189/1‑71 (MQ=255)

                                                      cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAacac              >  1:4111320/1‑71 (MQ=255)

                                                      cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAacac              <  1:2323848/71‑1 (MQ=255)

                                                      cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAacac              >  1:2925148/1‑71 (MQ=255)

                                                        gcgcTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggta                             <  1:111725/54‑3 (MQ=12)

                                                         cgcTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggta                             <  1:4648563/53‑3 (MQ=12)

                                                         cgcTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggta                             <  1:1468219/53‑3 (MQ=12)

                                                         cgcTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggta                             <  1:1037542/53‑3 (MQ=12)

                                                         cgcAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAacac              >  1:5112888/1‑68 (MQ=255)

                                                         cgcAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAacac              >  1:1550717/1‑68 (MQ=255)

                                                          gctaCGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAg           <  1:2540851/67‑1 (MQ=255)

                                                          gctaCGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGa          <  1:3725503/68‑1 (MQ=255)

                                                          gcAACGCGACGGTAGTCACCGATGATAcggccacggcca                                          <  1:4708354/39‑1 (MQ=255)

                                                          gcAACGCGACGGTAGTCACCGATGATAcggccacggcca                                          <  1:2937721/39‑1 (MQ=255)

                                                          gcAACGCGACGGTAGTCACCGATGATAcggccacggcca                                          <  1:4342510/39‑1 (MQ=255)

                                                            aaCGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGAtt        <  1:2322149/71‑1 (MQ=255)

                                                            aaCGCGACGGTAGTCACCGATGATACGGCCAAGGCCATATGCATCTGGAAGAc                          >  1:3947898/1‑53 (MQ=39)

                                                            aaCGCGACGGTAGTCACAGATGATACGGCCAAGGCCATATGCATCTGGCAGAc                          >  1:1878734/1‑53 (MQ=255)

                                                             aCGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGAtt        >  1:343396/1‑70 (MQ=255)

                                                              cgcgGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACGGGTCAGCACGCCAGAtt        <  1:2976377/69‑1 (MQ=255)

                                                              cgcgGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCa                    >  1:2939260/1‑57 (MQ=12)

                                                              cgcgGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTa      >  1:1043154/1‑71 (MQ=255)

                                                              cgcgACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTa      <  1:3262171/71‑1 (MQ=255)

                                                              cgcgACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTa      >  1:1977826/1‑71 (MQ=255)

                                                               gcgACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTa      <  1:3497576/70‑1 (MQ=255)

                                                               gcgACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGCTCAGAACACCAGATTTAc     <  1:2408572/71‑1 (MQ=255)

                                                                  aCGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGc  <  1:1593060/71‑1 (MQ=255)

CTGCAGAGAAGTGAACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGC  >  Exported/609639‑609775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`