| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 609,786 | G→A | 30.1% | D156D (GAC→GAT) | pflB ← | pyruvate formate lyase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 609,786 | 0 | G | A | 30.1% | 154.6 / 74.6 | 103 | D156D (GAC→GAT) | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base G (45/27); new base A (14/17); total (59/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.11e-01 | |||||||||||
Only 100 of 109 total aligned reads displayed.
CGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTG > Exported/609716‑609852 | cGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATg < 1:269486/71‑1 (MQ=255) atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTc > 1:3351202/1‑71 (MQ=255) atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTc > 1:417548/1‑71 (MQ=255) atACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCg > 1:5603563/1‑70 (MQ=255) atACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCg < 1:1230781/70‑1 (MQ=255) ccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa < 1:119365/71‑1 (MQ=255) cacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaa < 1:609488/71‑1 (MQ=255) cacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa < 1:472761/70‑1 (MQ=255) cacggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaa < 1:5234997/71‑1 (MQ=255) acggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa > 1:1256356/1‑71 (MQ=255) acggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTa < 1:826443/69‑1 (MQ=255) acggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTa < 1:5222851/69‑1 (MQ=255) cggccaTATGCATCTGGCAGACCTTTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa > 1:2340349/1‑70 (MQ=255) cggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa > 1:1699264/1‑70 (MQ=255) cggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAAc > 1:5312540/1‑71 (MQ=255) cggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAAc > 1:2896784/1‑71 (MQ=255) ggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACg < 1:114108/71‑1 (MQ=255) ggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAAca < 1:5066423/71‑2 (MQ=255) ccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATc < 1:4805222/71‑1 (MQ=255) caTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCg < 1:1392433/71‑1 (MQ=255) tatGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa < 1:2781513/62‑1 (MQ=255) tatGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa < 1:4442888/71‑1 (MQ=255) tatGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa < 1:1112119/71‑1 (MQ=255) agccATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCaaa > 1:4424970/4‑70 (MQ=255) agccATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCaaa > 1:4223194/4‑70 (MQ=255) agccATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCaaa > 1:2467907/4‑70 (MQ=255) gccATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa > 1:35780/3‑62 (MQ=255) gccATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCaaat < 1:4228793/68‑2 (MQ=255) tCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATcc < 1:2465098/48‑1 (MQ=16) tCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATcc < 1:4288415/48‑1 (MQ=16) tCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATcc < 1:3380499/48‑1 (MQ=16) tCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCggtg < 1:3678607/52‑3 (MQ=16) tCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTa < 1:685436/56‑1 (MQ=255) tCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTa < 1:145809/56‑1 (MQ=255) tCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa < 1:5121823/56‑1 (MQ=255) cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc > 1:4247352/1‑71 (MQ=255) tGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa < 1:1846544/54‑1 (MQ=255) tGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCg > 1:3039087/1‑61 (MQ=255) tGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCt > 1:5075076/1‑71 (MQ=255) tGGCAGACCGGTCAGAACACCAGATTTACGG‑AACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTg > 1:2475998/1‑71 (MQ=255) cAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa > 1:1376146/1‑60 (MQ=255) cAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa > 1:4702682/1‑60 (MQ=255) cAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa > 1:3868989/1‑60 (MQ=255) cAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGT‑CGGAGTGTAAACGTCGaa > 1:4552518/1‑59 (MQ=255) aaaCCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGt > 1:1024750/3‑70 (MQ=255) aCCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTtgt < 1:183171/71‑1 (MQ=255) cGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTtatgg > 1:3710304/1‑70 (MQ=255) ggTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa < 1:3069347/45‑1 (MQ=255) gTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGccc > 1:5571530/1‑60 (MQ=12) gTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGccc > 1:4575174/1‑60 (MQ=12) gTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGccc > 1:4581481/1‑60 (MQ=12) gTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGccc > 1:4106300/1‑60 (MQ=12) gTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca > 1:1149188/1‑55 (MQ=255) gTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAac > 1:1333273/1‑54 (MQ=255) tCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa > 1:2025101/1‑45 (MQ=255) tCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa > 1:3094220/1‑45 (MQ=255) cAGAACACCAGATTTACGGCAACGCATGATGTCCGGAGTGTAAACGTCg < 1:417847/49‑1 (MQ=255) aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt > 1:4540961/1‑70 (MQ=255) gAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTTAACGTCGaa > 1:4909086/1‑49 (MQ=255) gAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa > 1:2810451/1‑49 (MQ=255) aaCACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca > 1:3661589/1‑50 (MQ=255) aaCACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTa > 1:1366394/1‑70 (MQ=255) ccAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGtt < 1:1475341/63‑1 (MQ=16) cAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGTTTTACg > 1:4448415/1‑67 (MQ=255) aGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGTTTTACg > 1:5020873/1‑66 (MQ=255) aGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTAtt > 1:4220959/1‑71 (MQ=255) gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca > 1:425310/1‑43 (MQ=255) gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca > 1:3553148/1‑43 (MQ=255) gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTAtt > 1:5335648/1‑70 (MQ=255) gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTc < 1:2987674/71‑1 (MQ=255) tttACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGa > 1:5605969/1‑38 (MQ=255) tttAAGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa > 1:5282951/1‑39 (MQ=255) tACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGTTTTACg > 1:624521/1‑61 (MQ=16) tACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGa < 1:5670167/53‑1 (MQ=255) tACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGa < 1:718323/53‑1 (MQ=255) aCGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTg > 1:4481659/1‑70 (MQ=255) gCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGa < 1:257130/49‑1 (MQ=255) cAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGtttt > 1:1238475/1‑53 (MQ=255) aaCGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAtt > 1:3581036/1‑71 (MQ=255) aCGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTg > 1:2324190/1‑64 (MQ=255) aCGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTg < 1:2607159/64‑1 (MQ=255) cGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAtttt < 1:1350574/71‑1 (MQ=255) gCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTa > 1:708863/1‑50 (MQ=255) gCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTa > 1:1039979/1‑50 (MQ=255) gCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAttttt < 1:1592137/71‑1 (MQ=255) cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACg > 1:3635746/1‑51 (MQ=255) cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACg > 1:683568/1‑51 (MQ=255) cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACg > 1:1739280/1‑51 (MQ=255) cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACg > 1:2460056/1‑51 (MQ=255) cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAtttttt > 1:1047864/1‑71 (MQ=255) cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAtttttt > 1:2801026/1‑71 (MQ=255) cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAttttt < 1:5433830/70‑1 (MQ=255) ggATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAg < 1:3102581/42‑1 (MQ=255) ggATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCGGTGAAGATTTTTTTg < 1:1513943/71‑1 (MQ=255) gATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTa < 1:3490918/46‑1 (MQ=255) gTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGt < 1:4448383/39‑1 (MQ=255) gTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGt < 1:166337/39‑1 (MQ=255) gTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGt < 1:1694819/39‑1 (MQ=255) gTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGt < 1:3298447/39‑1 (MQ=255) gTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGt < 1:2950033/39‑1 (MQ=255) | CGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTG > Exported/609716‑609852 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |