| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 609,807 | C→T | 17.5% | V149V (GTG→GTA) | pflB ← | pyruvate formate lyase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 609,807 | 0 | C | T | 17.5% | 174.6 / 23.5 | 74 | V149V (GTG→GTA) | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base C (28/33); new base T (9/4); total (37/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGATCCAGTTCGCGGTT > Exported/609738‑609875 | tGCATCTGGCAGACCGGGCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca > 1:3822322/1‑71 (MQ=255) cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc > 1:4247352/1‑71 (MQ=255) tGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCt > 1:5075076/1‑71 (MQ=255) tGGCAGACCGGTCAGAACACCAGATTTACGG‑AACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTg > 1:2475998/1‑71 (MQ=255) aaaCCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGt > 1:1024750/3‑70 (MQ=255) aCCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTtgt < 1:183171/71‑1 (MQ=255) cGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGcc < 1:870396/61‑1 (MQ=16) cGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTtatgg > 1:3710304/1‑70 (MQ=255) gTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGccc > 1:4106300/1‑60 (MQ=12) gTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGccc > 1:5571530/1‑60 (MQ=12) gTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGccc > 1:4575174/1‑60 (MQ=12) gTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGccc > 1:4581481/1‑60 (MQ=12) gTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca > 1:1149188/1‑55 (MQ=255) gTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAac > 1:1333273/1‑54 (MQ=255) aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt > 1:4540961/1‑70 (MQ=255) aaCACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca > 1:3661589/1‑50 (MQ=255) aaCACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTa > 1:1366394/1‑70 (MQ=255) ccAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGtt < 1:1475341/63‑1 (MQ=16) ccAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGTTTTACgcag < 1:5526446/71‑4 (MQ=255) cAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGTTTTACg > 1:4448415/1‑67 (MQ=255) aGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGTTTTACg > 1:5020873/1‑66 (MQ=255) aGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTAtt > 1:4220959/1‑71 (MQ=255) gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca > 1:425310/1‑43 (MQ=255) gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca > 1:3553148/1‑43 (MQ=255) gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTAtt > 1:5335648/1‑70 (MQ=255) gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTc < 1:2987674/71‑1 (MQ=255) aTTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGt < 1:1816085/58‑1 (MQ=12) tACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGTTTTACg > 1:624521/1‑61 (MQ=16) tACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGa < 1:5670167/53‑1 (MQ=255) tACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGa < 1:718323/53‑1 (MQ=255) aCGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTg > 1:4481659/1‑70 (MQ=255) gCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGa < 1:257130/49‑1 (MQ=255) cAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGtttt > 1:1238475/1‑53 (MQ=255) aaCGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAtt > 1:3581036/1‑71 (MQ=255) aCGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTg > 1:2324190/1‑64 (MQ=255) aCGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTg < 1:2607159/64‑1 (MQ=255) cGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAtttt < 1:1350574/71‑1 (MQ=255) gCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTa > 1:708863/1‑50 (MQ=255) gCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTa > 1:1039979/1‑50 (MQ=255) gCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAttttt < 1:1592137/71‑1 (MQ=255) cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACg > 1:683568/1‑51 (MQ=255) cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACg > 1:3635746/1‑51 (MQ=255) cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACg > 1:2670501/1‑51 (MQ=255) cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACg > 1:2460056/1‑51 (MQ=255) cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACg > 1:1739280/1‑51 (MQ=255) cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAtttttt > 1:1047864/1‑71 (MQ=255) cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAtttttt > 1:2801026/1‑71 (MQ=255) cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAttttt < 1:5433830/70‑1 (MQ=255) ggATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAg < 1:3102581/42‑1 (MQ=255) ggATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCGGTGAAGATTTTTTTg < 1:1513943/71‑1 (MQ=255) ggATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTg < 1:3189165/71‑1 (MQ=255) gATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTa < 1:3490918/46‑1 (MQ=255) gTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGt < 1:3298447/39‑1 (MQ=255) gTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGt < 1:166337/39‑1 (MQ=255) gTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGt < 1:1694819/39‑1 (MQ=255) gTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGt < 1:4448383/39‑1 (MQ=255) gTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGt < 1:2950033/39‑1 (MQ=255) ccGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAttttt < 1:4760888/62‑1 (MQ=255) cGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt < 1:1222441/38‑1 (MQ=255) cGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTc < 1:4623315/48‑1 (MQ=255) cGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAttttt < 1:1991010/61‑1 (MQ=255) gAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAttttttt < 1:2310444/61‑1 (MQ=255) aGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAttttttt < 1:210451/60‑1 (MQ=255) tAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGa > 1:4853562/1‑46 (MQ=255) tAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGatc > 1:4175961/1‑60 (MQ=255) tAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGa > 1:1071532/1‑58 (MQ=255) tAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGATCCa < 1:4763167/70‑1 (MQ=255) aaaCGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCgg < 1:64306/64‑1 (MQ=255) aCGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGatcat < 1:3468678/59‑1 (MQ=255) aCGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGatcat < 1:1853019/59‑1 (MQ=255) aCGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGATCCAGTTc > 1:5094778/1‑71 (MQ=255) aCGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGATCCAGTTc < 1:1604129/71‑1 (MQ=255) gTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGatcat > 1:5603145/1‑57 (MQ=255) gTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGatcat > 1:4820410/1‑57 (MQ=255) gTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCg > 1:2703148/1‑59 (MQ=255) gTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCg > 1:3815200/1‑59 (MQ=255) tCGAACACGCCCTGGTTGTGAGTTTTACGGTATTc < 1:2157885/35‑1 (MQ=255) tCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAtttt < 1:694077/47‑1 (MQ=255) tCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAtttt < 1:1833171/47‑1 (MQ=255) aaCACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGATCCAGTTCGCGGtt < 1:2846060/71‑1 (MQ=255) acacGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGATCCa > 1:1178933/1‑60 (MQ=255) cacGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTg < 1:1811448/46‑1 (MQ=255) | TGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTGATCATCGGATCCAGTTCGCGGTT > Exported/609738‑609875 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |