| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 704,276 | T→C | 29.3% | L123L (CTA→CTG) | ymcA ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 704,276 | 0 | T | C | 29.3% | 136.5 / 66.0 | 92 | L123L (CTA→CTG) | ymcA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (33/32); new base C (18/9); total (51/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
AGATACTCACCGTCAAACAGACCCGTACCAGCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGC > Exported/704214‑704343 | aGATACTCACCGTCAAACAGACCCGTACCAGCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGt > 1:2073394/1‑70 (MQ=255) aCTCACCGTCAAACAGACCCGTACCAGCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAAcc < 1:3380847/70‑1 (MQ=255) cTCACCGTCAAACAGACCCGTACCAGCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACct < 1:1326816/70‑1 (MQ=255) aCCGTCAAACAGACCCGTACCAGCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACcttctt > 1:2573815/1‑71 (MQ=255) aaaCAGCCCCGTACCGCCGATATCCCGCGCACCAACCGCCACTTGCGGCAGCCAGTaa > 1:3500398/1‑58 (MQ=255) aaaCAGCCCCGTACCGCCGATATCCCGCGCACCAACCGCCACTTGCGGCAGCCAGTaa > 1:2777041/1‑58 (MQ=255) aaaCAGCCCCGTACCGCCGATATCCCGCGCACCAACCGCCACTTGCGGCAGCCAGTAAct > 1:1872842/1‑59 (MQ=255) aaaCAGCCCCGTACCGCCGATATCCCGCGCACCAACCGCCACTTGCGGCAGCCAGTAACTCTCTTCCcac > 1:497847/1‑70 (MQ=255) aaaCAGACCCGTACCAGCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGTaa > 1:3834859/1‑58 (MQ=255) aaaCAGACCCGTACCAGCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCca > 1:3045559/1‑69 (MQ=255) aCAGCCCCGTACCGCCGATATCCCGCGCACCAACCGCCACTTGCGGCAGCCAGTAACTCTCTTCCca > 1:2056560/1‑67 (MQ=255) cAGACCCGTACCAGCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGc > 1:2162574/1‑71 (MQ=255) aGACCCGTACCAGCAATATCACGTTTACCAAACGCCACTTGCGGt < 1:3471653/45‑1 (MQ=255) aGACCCGTACCAGCAATATCACGTTTACCAAACGCCACTTGCGGt < 1:2973130/45‑1 (MQ=255) gTACCAGCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGtt > 1:4853548/1‑70 (MQ=255) aCCAGCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGTaa > 1:3471704/1‑46 (MQ=255) ccAGCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGt > 1:3252915/1‑43 (MQ=255) ccAGCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGaa > 1:1735024/1‑64 (MQ=255) cAGCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCca < 1:1845524/55‑1 (MQ=255) cAGCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTaaa < 1:4148528/70‑1 (MQ=255) aGCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGaa > 1:2754284/1‑62 (MQ=255) gCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGTGAc > 1:3648486/1‑43 (MQ=255) gCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATc < 1:1958778/71‑1 (MQ=255) tatCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACcttc < 1:2170687/43‑1 (MQ=255) tatCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTccc < 1:4437676/48‑1 (MQ=255) tatCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTccc < 1:1352663/48‑1 (MQ=255) tatCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTccc < 1:307749/48‑1 (MQ=255) tatCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCg < 1:4213472/55‑1 (MQ=255) tatCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCg < 1:138198/55‑1 (MQ=255) atCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGaa > 1:2059709/1‑69 (MQ=255) atCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGaa > 1:4044390/1‑69 (MQ=255) tCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAAc > 1:1106530/1‑37 (MQ=255) tCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTaa < 1:5641687/61‑1 (MQ=255) tCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAAt > 1:4654760/1‑69 (MQ=255) aCGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAAt > 1:2205433/1‑62 (MQ=255) aCGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGAtt > 1:4414167/1‑71 (MQ=255) aCGTTTACCAAACGCAACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAAt > 1:3409610/1‑62 (MQ=255) cGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAgg < 1:473230/48‑1 (MQ=255) gTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCg < 1:2419561/49‑1 (MQ=255) gTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCg < 1:4828791/49‑1 (MQ=255) gTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTg > 1:2473338/1‑71 (MQ=255) tttACCAAACGCCACTTGCGGTAGCCAGTAACcttctt < 1:573403/38‑1 (MQ=255) tttACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGa < 1:2828585/49‑1 (MQ=255) ttACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTaaaa > 1:311831/1‑57 (MQ=255) tACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGAttt < 1:4087923/67‑1 (MQ=255) aCCAACCGCCACTTGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGTTTGAGATCGAAGGCTTTATCtt < 1:5372011/71‑1 (MQ=255) ccAACCGCCACTTGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGTTTGAGATCGAAGGCTTTATCtt > 1:985316/1‑70 (MQ=255) ccAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCtt < 1:2763889/70‑1 (MQ=255) cAACCGCCACTTGCGGCAGCCAGTAACTCTCTTcc > 1:5032234/1‑35 (MQ=16) cAACCGCCACTTGCGGCAGCCAGTAACTCTCTTcc > 1:5094348/1‑35 (MQ=16) cAACCGCCACTTGCGGCAGCCAGTAACTCTCTTcc > 1:2937466/1‑35 (MQ=16) cAACCGCCACTTGCGGCAGCCAGTAACTCTCTTcc > 1:2131396/1‑35 (MQ=16) cAACCGCCACTTGCGGCAGCCAGTAACTCTCTTcc > 1:1365190/1‑35 (MQ=16) cAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCcac > 1:2383066/1‑38 (MQ=255) cAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTaaaa > 1:3623456/1‑53 (MQ=255) cAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGAttt < 1:4834842/64‑1 (MQ=255) cAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTg < 1:2303699/71‑1 (MQ=255) cAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTg < 1:4194489/71‑1 (MQ=255) aaaCGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGt < 1:5401449/71‑1 (MQ=255) accgcgACTTGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGTTTGAGATCGAAGGCTTTATCTTtata > 1:4471478/3‑71 (MQ=255) accGCCACTTGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGTTTGAGATCGAAGGCTTTATCTTtata > 1:3816527/3‑71 (MQ=255) ccGCCACTTGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGttt < 1:4133729/45‑1 (MQ=16) ccGCCACTTGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGttt < 1:1437401/45‑1 (MQ=16) ccGCCACTTGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGttt < 1:1650483/45‑1 (MQ=16) ccGCCACTTGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGttt < 1:5020055/45‑1 (MQ=16) ccGCCACTTGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGttt < 1:2811956/45‑1 (MQ=16) cGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGATTGATTTGTCTTTGTATg < 1:2826470/71‑1 (MQ=255) gCCACTTGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGttt > 1:3198555/1‑44 (MQ=16) ccACTTGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGtt > 1:5680876/1‑42 (MQ=16) aCTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTg < 1:298368/71‑1 (MQ=255) cTTGCGGTAGCCAGTAACCTTCTTCCCAGAGGCGAAGttt > 1:5490348/1‑40 (MQ=255) cTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGttt > 1:5004219/1‑40 (MQ=255) cTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGa < 1:4845245/71‑1 (MQ=255) ttGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTaaa < 1:603560/42‑1 (MQ=255) ttGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTaaa < 1:4581591/42‑1 (MQ=255) ttGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTaa < 1:1533551/41‑1 (MQ=255) ttGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTaa < 1:2062117/41‑1 (MQ=255) ttGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTaa < 1:279047/41‑1 (MQ=255) ttGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCttt > 1:2282672/1‑60 (MQ=255) ttGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCttt > 1:1376976/1‑60 (MQ=255) ttGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGTTTGAGATCGAAGGCTTTATc < 1:4057529/57‑1 (MQ=255) tGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCt > 1:3091942/1‑57 (MQ=255) tGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGTTTGAGATCGAAGGCTTTATCTTTATACGTTTGATc > 1:1750856/1‑71 (MQ=255) tGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGTTTGAGATCGAAGGCTTTATCTTTATACGTTTGATc > 1:3675203/1‑71 (MQ=255) tGCGGCAGCCAGTAACTCACTTCCCACAAACGCAGATTGAGATCGAAGGCTTTATCTTtata > 1:3758542/1‑62 (MQ=255) gCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAAt > 1:570752/1‑42 (MQ=255) gCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAAt > 1:5088302/1‑42 (MQ=255) gCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAAt > 1:4923259/1‑42 (MQ=255) gCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGaa > 1:1240711/1‑46 (MQ=255) gCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGaa > 1:3399473/1‑46 (MQ=255) gCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATcg > 1:2796996/1‑71 (MQ=255) cGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATcg < 1:2704464/70‑1 (MQ=255) cGGCAGCCAGTAACTCTCTTCCCACAAACGCAGTTTGAGATCGAAGGCTTTATCTTTATACGTTTGATcgc < 1:4096640/71‑1 (MQ=255) cGGCAGCCAGTAACTCTCTTCCCACAAACGCAGTTTGAGATCGAAGGCTTTATCTTTATACGTTTGATcgc < 1:3651759/71‑1 (MQ=255) tAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTg < 1:1210079/55‑1 (MQ=255) tAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTg < 1:2474168/55‑1 (MQ=255) tAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTg < 1:4445834/55‑1 (MQ=255) tAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTg < 1:1648098/55‑1 (MQ=255) | AGATACTCACCGTCAAACAGACCCGTACCAGCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGC > Exported/704214‑704343 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |