Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 705,295 | A→G | 65.3% | G32G (GGT→GGC) | ymcB ← | conserved hypothetical protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 705,295 | 0 | A | G | 65.3% | 101.0 / 84.1 | 98 | G32G (GGT→GGC) | ymcB | conserved hypothetical protein |
Reads supporting (aligned to +/- strand): ref base A (12/22); new base G (35/29); total (47/51) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 8.97e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 |
GTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGC > Exported/705231‑705359 | gTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAgg > 1:2467759/1‑70 (MQ=255) gTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAgg > 1:1323517/1‑70 (MQ=255) ttGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGcc < 1:4646164/66‑1 (MQ=255) gCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAgg > 1:2067206/1‑67 (MQ=255) cGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGAt < 1:3325474/71‑1 (MQ=255) cGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGAt < 1:5082063/71‑1 (MQ=255) ggTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGa > 1:3525173/1‑69 (MQ=255) gTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATa > 1:1706304/1‑70 (MQ=255) ttGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATaaa > 1:5593110/1‑71 (MQ=255) ttGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATaaa < 1:2062256/71‑1 (MQ=255) tgtgTCACCAACTGGAGAACATTTTCCACGGGGCCAAAGGAAAGTGTCTGTTGTTCACCAGGCAGATaaa < 1:5455278/70‑1 (MQ=255) tgtgTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATaaa > 1:2814589/1‑70 (MQ=255) gtgtCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATaaa > 1:4949900/1‑69 (MQ=255) gtCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGt > 1:2948676/1‑71 (MQ=255) aCCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGt > 1:2400943/1‑68 (MQ=255) aCCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCAc > 1:1826013/1‑71 (MQ=255) aCCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCAcc > 1:1668538/1‑53 (MQ=255) cTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGAt < 1:5049762/56‑1 (MQ=255) cTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTc < 1:3369428/71‑1 (MQ=255) gACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCtt > 1:3873449/1‑71 (MQ=255) gACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCtt > 1:5235950/1‑71 (MQ=255) gACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCt < 1:5546400/70‑1 (MQ=255) gACAACATTTTCCACGGGGCCAACGGAAAGTGTCTCTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCt < 1:4351207/70‑1 (MQ=255) caacaTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTc < 1:5399677/59‑1 (MQ=255) aacaTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTgcg > 1:2601331/1‑71 (MQ=255) aacaTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTgcg > 1:4078041/1‑71 (MQ=255) caTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCa < 1:3530549/71‑1 (MQ=255) caTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCa < 1:2955657/71‑1 (MQ=255) caTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCAc > 1:3232478/1‑39 (MQ=255) caTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCa < 1:4272377/71‑1 (MQ=255) caTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCa < 1:3088228/71‑1 (MQ=255) aTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCaa < 1:1279392/71‑1 (MQ=255) aTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCa > 1:335286/1‑70 (MQ=255) ttttCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATa > 1:1830588/1‑51 (MQ=255) ttttCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATa > 1:3971430/1‑51 (MQ=255) ttttCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATa > 1:4354141/1‑51 (MQ=255) ttttCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCaaa < 1:1024238/71‑1 (MQ=255) ttttCCACGGGGCCAACGCAAAGTGTCTGTTGTTCGCCAGGCAGATAAATa > 1:3409565/1‑51 (MQ=255) tttCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCaaa < 1:45473/70‑1 (MQ=255) ttCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCa > 1:560286/1‑37 (MQ=255) ttCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTgcg < 1:2325207/65‑1 (MQ=255) ccACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGc < 1:5039818/70‑1 (MQ=255) cACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGc < 1:1825760/69‑1 (MQ=255) cACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGc < 1:777315/69‑1 (MQ=255) cACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCAt < 1:890948/71‑1 (MQ=255) aCGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCa < 1:248241/63‑1 (MQ=255) cGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAATGCATgg < 1:740320/71‑1 (MQ=255) cGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATgg < 1:470969/71‑1 (MQ=255) cGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATgg < 1:5680/71‑1 (MQ=255) cGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATgg < 1:3560483/71‑1 (MQ=255) cGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATgg < 1:1854651/71‑1 (MQ=255) cGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATgg < 1:2727676/71‑1 (MQ=255) cGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATgg < 1:2969537/71‑1 (MQ=255) ggggCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATggg < 1:215805/71‑1 (MQ=255) ccAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGgtgt < 1:4442702/71‑1 (MQ=255) aaCGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCa > 1:3774361/1‑71 (MQ=255) aaCGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCa < 1:5450323/71‑1 (MQ=255) aCGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCa > 1:451548/1‑70 (MQ=255) aCGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCa > 1:1364887/1‑70 (MQ=255) aCGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCAt > 1:189995/1‑71 (MQ=255) aCGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCAt < 1:3336826/71‑1 (MQ=255) aCGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCAt < 1:2637877/71‑1 (MQ=255) aCGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTTACCGTTCCTTGCGCAAAGGCATGGGGTGTCAt < 1:2653506/71‑1 (MQ=255) aCGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCa < 1:286123/60‑1 (MQ=255) aCGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCa < 1:4752110/60‑1 (MQ=255) aCGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCa < 1:5085695/60‑1 (MQ=255) aCGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCa < 1:3077881/60‑1 (MQ=255) aCGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCa < 1:702857/60‑1 (MQ=255) aCGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCAt < 1:1454744/71‑1 (MQ=255) aCGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCAt > 1:5073611/1‑71 (MQ=255) cGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTcc > 1:2001150/1‑46 (MQ=255) cGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTcc > 1:4677834/1‑46 (MQ=255) ggAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAg > 1:465907/1‑35 (MQ=255) aaaGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCa > 1:3802822/1‑66 (MQ=255) aaaGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACg < 1:580404/71‑1 (MQ=255) aGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTa < 1:4032273/71‑1 (MQ=255) gtgtCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCAtt < 1:3854563/65‑1 (MQ=255) gtCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAg > 1:3592397/1‑48 (MQ=255) tCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAg < 1:5053415/70‑1 (MQ=255) tgttgtTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCaaa > 1:3158827/1‑44 (MQ=255) tgttgtTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCaaa > 1:640365/1‑44 (MQ=255) tgttgtTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCa > 1:4782307/1‑42 (MQ=255) tgttgtTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTaaa > 1:5037737/1‑67 (MQ=255) tgttgtTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTaaa > 1:4238258/1‑67 (MQ=255) ttgttCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATgggg > 1:3426767/1‑51 (MQ=255) ttgttCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGgtg > 1:4623417/1‑53 (MQ=255) ttgttCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGgtg > 1:4350422/1‑53 (MQ=255) ttgttCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGgtg > 1:3420302/1‑53 (MQ=255) ttgttCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTaa < 1:1570900/64‑1 (MQ=255) ttgttCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTaa < 1:3507836/64‑1 (MQ=255) ttgttCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCa < 1:2664524/40‑1 (MQ=255) ttgttCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCa < 1:462931/40‑1 (MQ=255) ttgttCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGc > 1:1222528/1‑71 (MQ=255) tgttCACCAGGCAGATAAATAGTCACCGATCCTTgc > 1:541104/1‑36 (MQ=255) ttCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTc < 1:261186/52‑1 (MQ=255) ttCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTaaa > 1:1754968/1‑62 (MQ=255) cACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCAt > 1:3565230/1‑42 (MQ=255) cACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCAt > 1:3119351/1‑42 (MQ=255) | GTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGC > Exported/705231‑705359 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |