Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 706,680 | C→T | 68.0% | R244K (AGG→AAG) | lpxL ← | lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 706,680 | 0 | C | T | 68.0% | 85.5 / 57.9 | 75 | R244K (AGG→AAG) | lpxL | lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase |
Reads supporting (aligned to +/- strand): ref base C (11/13); new base T (25/26); total (36/39) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 8.10e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGT > Exported/706611‑706750 | tCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGcc > 1:405395/1‑70 (MQ=255) tCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGcc > 1:4435626/1‑70 (MQ=255) ggCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTAc < 1:3330071/71‑1 (MQ=255) ggCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTAc > 1:683211/1‑71 (MQ=255) gCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACg < 1:1480346/71‑1 (MQ=255) atcatcCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGc < 1:1499191/71‑1 (MQ=255) atcCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGt < 1:5252444/70‑1 (MQ=255) atcCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTg < 1:5070866/71‑1 (MQ=255) tcCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTgg < 1:1929517/71‑1 (MQ=255) tcCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTg > 1:4177524/1‑70 (MQ=255) cAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTAc > 1:5202729/1‑62 (MQ=255) cAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGaa > 1:771878/1‑71 (MQ=255) gTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCATTTGCCATCTGGCTTa < 1:230162/59‑1 (MQ=255) ggCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACg > 1:3848430/1‑69 (MQ=255) gagaACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACg < 1:4786069/65‑1 (MQ=255) gaACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGt > 1:3396214/1‑57 (MQ=255) aaCACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGaa < 1:2719641/64‑1 (MQ=255) aaCACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGaa < 1:2869639/64‑1 (MQ=255) cacTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCAc > 1:2065220/1‑68 (MQ=255) cacTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAg < 1:2078512/71‑1 (MQ=255) ctGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACAt > 1:3312363/1‑71 (MQ=255) ctGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACAt > 1:1433572/1‑71 (MQ=255) tGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATg > 1:2262814/1‑71 (MQ=255) tGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTgg < 1:4540582/51‑1 (MQ=255) tGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTgg < 1:3965942/51‑1 (MQ=255) tGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTgg < 1:2592606/51‑1 (MQ=255) tGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATg > 1:859981/1‑71 (MQ=255) tGGCGGCAGCATAATCAATTGATACCCTATGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATg > 1:1003549/1‑71 (MQ=255) gcggcAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATgcg < 1:870674/71‑1 (MQ=255) gcggcAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATgc < 1:2143725/70‑1 (MQ=255) gcggcAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATgc < 1:4120916/70‑1 (MQ=255) ggcAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATgcgc < 1:3707976/70‑1 (MQ=255) gcaTAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAggg < 1:5687728/52‑1 (MQ=255) caTAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCAc < 1:5686564/54‑1 (MQ=255) caTAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCAc < 1:1332793/54‑1 (MQ=255) aTAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACAt > 1:1883669/1‑71 (MQ=255) tAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATc < 1:1732411/71‑1 (MQ=255) tAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATc < 1:498099/71‑1 (MQ=255) tAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATc < 1:1488989/71‑1 (MQ=255) tAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGc > 1:29518/1‑50 (MQ=255) aTCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACa > 1:492608/1‑56 (MQ=255) aTCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACa > 1:5666328/1‑56 (MQ=255) aTCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACa > 1:2173836/1‑56 (MQ=255) aTCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACa > 1:1464473/1‑56 (MQ=255) aTCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACa > 1:252752/1‑56 (MQ=255) aTCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACa > 1:2489365/1‑56 (MQ=255) aTCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACa > 1:4166294/1‑56 (MQ=255) aTCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCg > 1:4814013/1‑71 (MQ=255) aTCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCg > 1:67273/1‑71 (MQ=255) ttGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACAt < 1:5338626/52‑1 (MQ=255) ttGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACAt < 1:1889321/52‑1 (MQ=255) ttGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGc > 1:1376289/1‑69 (MQ=255) gATACCCTTTGCCATCTGGCTTACGGCGTGGAACGa > 1:1777895/1‑36 (MQ=255) gATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACAt > 1:2483225/1‑50 (MQ=255) gATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACAt > 1:2055499/1‑50 (MQ=255) aTACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGc < 1:3215081/70‑1 (MQ=255) aTACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCa > 1:421786/1‑71 (MQ=255) cccTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATcc < 1:4090322/71‑1 (MQ=255) cccTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATc < 1:5008766/70‑1 (MQ=255) cccTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATc < 1:2598863/70‑1 (MQ=255) cccTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATc < 1:3551589/70‑1 (MQ=255) ccTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATc < 1:4203195/69‑1 (MQ=255) gCCATCTGGCCTACGGCGTGGAACGAAGGGCA‑CAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTc < 1:574531/70‑1 (MQ=255) aTCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCg < 1:4088636/43‑1 (MQ=255) aTCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGa < 1:2283814/45‑1 (MQ=255) aTCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGa < 1:3267296/45‑1 (MQ=255) aTCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATc < 1:3603419/49‑1 (MQ=255) aTCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGt < 1:1354604/66‑1 (MQ=255) aTCTGGCTTACGGCGTGGAACGAAGGGCACAAGACATGCGCCGGACATCCGTGCCAGCATCCAGGt < 1:4751659/66‑1 (MQ=255) cTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGa > 1:1847570/1‑43 (MQ=255) cTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGa > 1:4952296/1‑43 (MQ=255) cTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGa > 1:3954867/1‑43 (MQ=255) cTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACa > 1:821757/1‑45 (MQ=255) tGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCg > 1:3304611/1‑71 (MQ=255) tGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCg > 1:5241956/1‑71 (MQ=255) tGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCAAGCATCCAGGTTCCGGTCg > 1:4517339/1‑71 (MQ=255) tGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGACAGCATCCAGGTTCCGGTCg > 1:4353489/1‑71 (MQ=255) ccTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTgg > 1:397122/1‑71 (MQ=255) cTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGt < 1:2555206/71‑1 (MQ=255) | TCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGT > Exported/706611‑706750 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |