Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 756,326 C→G 14.3% R151R (CGC→CGG) ‡ lolD → outer membrane‑specific lipoprotein transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported756,3260CG14.3% 115.1 / 9.1 42R151R (CGC→CGG) ‡lolDouter membrane‑specific lipoprotein transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (22/14);  new base G (4/2);  total (26/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTTAAAAGCGGTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTA  >  Exported/756259‑756396
                                                                   |                                                                      
tGTTAAAAGCGGTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAg                                                                     <  1:3312325/71‑1 (MQ=255)
    aaaaGCGGTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCgtg                                                                 >  1:2557694/1‑71 (MQ=255)
     aaaGCGGTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCgtgt                                                                >  1:1230763/1‑71 (MQ=255)
        gCGGTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGc                                                             <  1:3660592/71‑1 (MQ=255)
                gCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGa                                                           <  1:1465715/65‑1 (MQ=255)
                gCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGccc                                                     >  1:4117877/1‑71 (MQ=255)
                 cTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCg                                                    >  1:532499/1‑71 (MQ=255)
                      tcatcGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTgcgc                                               >  1:4637102/1‑71 (MQ=255)
                         acGTGCACATCACTACCCTTCCGAACTTTCTGGTGGTCAACAGCAGCGTGTGGCGATTGCCCGCGCGCTgg                                            <  1:1602388/70‑1 (MQ=9)
                          cGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCgtg                                                                 >  1:5556916/1‑49 (MQ=255)
                          cGTGCACATCACTACCCTTCCGAACTTTCTGGTGGTCAACAGCAGCGTGTGGCGATTGCCCGCGCGCTggc                                           >  1:4738873/1‑70 (MQ=9)
                               gAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGa                                                           >  1:3205053/1‑50 (MQ=255)
                                caTCACTACCCTTCCGAACTTTCTGGTGGTCAACAGCAGCGTGTGGCGATTGccc                                                     >  1:3741188/2‑55 (MQ=9)
                                caTCACTACCCTTCCGAACTTTCTGGTGGTCAACAGCAGCGTGTGGCGATTGccc                                                     >  1:2360772/2‑55 (MQ=9)
                                aaTCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATaa                                     <  1:295227/71‑1 (MQ=255)
                                 aTCACTACCCTTCCGAACTTTCTGGTGGTCAACAGCAGCGTGTGGCGATTGCCCgcg                                                  >  1:5039457/1‑55 (MQ=9)
                                 aTCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAAc                                    <  1:2119147/71‑1 (MQ=255)
                                  tCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAAcc                                   >  1:110642/1‑71 (MQ=255)
                                    aCCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCgt                                                                  >  1:799255/1‑38 (MQ=255)
                                    aCCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCcg                                 >  1:2491998/1‑71 (MQ=255)
                                     ccGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCcgc                                <  1:2408147/71‑1 (MQ=255)
                                      cGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCcgcg                               >  1:5257174/1‑71 (MQ=255)
                                      cGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCcgc                                >  1:297353/1‑70 (MQ=255)
                                        cccATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCTATTGCCCGTCCGCTGGTGAATAACCCGCGcc                             <  1:747219/71‑1 (MQ=255)
                                        cccATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGcc                             <  1:3835882/71‑1 (MQ=255)
                                              tGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGccc                                                     >  1:3982004/1‑41 (MQ=255)
                                              tGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGt                                           >  1:449435/1‑51 (MQ=255)
                                              tGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGt                                           >  1:3204423/1‑51 (MQ=255)
                                              tGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGt                                           >  1:4986793/1‑51 (MQ=255)
                                               gAACTTTCTGGTGGTCAACAGCAGCGTGTGGCGATTGCCCgcg                                                  <  1:2434335/43‑3 (MQ=9)
                                               gAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTgg                          <  1:2890172/67‑1 (MQ=255)
                                                aaCTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGt                         <  1:5209298/67‑1 (MQ=255)
                                                aaCTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGt                         <  1:4163726/67‑1 (MQ=255)
                                                aaCTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGt                         <  1:5636335/67‑1 (MQ=255)
                                                aaCTTTCTGGCGGCGAACGCCAGCCTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTg                     >  1:1534050/1‑71 (MQ=255)
                                                 aCTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTgg                    >  1:4669592/1‑71 (MQ=255)
                                                  cTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTAc                       >  1:1533487/1‑67 (MQ=255)
                                                  cTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGACCGTGCGCTGGTGAATAACCCGCGCCTGGTAc                       >  1:430245/1‑67 (MQ=255)
                                                     tCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCgg                 <  1:5689915/70‑1 (MQ=255)
                                                     tCTGGCGGCGAACGCCAGCGTGTGGCGATTGACCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGa                >  1:920414/1‑71 (MQ=255)
                                                         gcggcgAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGa             <  1:4567061/70‑1 (MQ=255)
                                                                aCGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCg     >  1:3649286/1‑71 (MQ=255)
                                                                   ccAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTa  <  1:3328664/71‑1 (MQ=255)
                                                                   |                                                                      
TGTTAAAAGCGGTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTA  >  Exported/756259‑756396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: