| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 756,347 | T→C | 27.6% | R158R (CGT→CGC) | lolD → | outer membrane‑specific lipoprotein transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 756,347 | 0 | T | C | 27.6% | 68.6 / 28.9 | 47 | R158R (CGT→CGC) | lolD | outer membrane‑specific lipoprotein transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (18/16); new base C (2/11); total (20/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.45e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
TCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCC > Exported/756281‑756416 | tcatcGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTgcgc > 1:4637102/1‑71 (MQ=255) acGTGCACATCACTACCCTTCCGAACTTTCTGGTGGTCAACAGCAGCGTGTGGCGATTGCCCGCGCGCTgg < 1:1602388/70‑1 (MQ=9) cGTGCACATCACTACCCTTCCGAACTTTCTGGTGGTCAACAGCAGCGTGTGGCGATTGCCCGCGCGCTggc > 1:4738873/1‑70 (MQ=9) aaTCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATaa < 1:295227/71‑1 (MQ=255) aTCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAAc < 1:2119147/71‑1 (MQ=255) tCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAAcc > 1:110642/1‑71 (MQ=255) aCCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCcg > 1:2491998/1‑71 (MQ=255) ccGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCcgc < 1:2408147/71‑1 (MQ=255) cGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCcgcg > 1:5257174/1‑71 (MQ=255) cGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCcgc > 1:297353/1‑70 (MQ=255) cccATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCTATTGCCCGTCCGCTGGTGAATAACCCGCGcc < 1:747219/71‑1 (MQ=255) cccATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGcc < 1:3835882/71‑1 (MQ=255) tGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGt > 1:449435/1‑51 (MQ=255) tGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGt > 1:3204423/1‑51 (MQ=255) tGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGt > 1:4986793/1‑51 (MQ=255) gAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTgg < 1:2890172/67‑1 (MQ=255) aaCTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGt < 1:5209298/67‑1 (MQ=255) aaCTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGt < 1:5636335/67‑1 (MQ=255) aaCTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGt < 1:4163726/67‑1 (MQ=255) aaCTTTCTGGCGGCGAACGCCAGCCTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTg > 1:1534050/1‑71 (MQ=255) aCTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTgg > 1:4669592/1‑71 (MQ=255) cTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTAc > 1:1533487/1‑67 (MQ=255) cTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGACCGTGCGCTGGTGAATAACCCGCGCCTGGTAc > 1:430245/1‑67 (MQ=255) tCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCgg < 1:5689915/70‑1 (MQ=255) tCTGGCGGCGAACGCCAGCGTGTGGCGATTGACCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGa > 1:920414/1‑71 (MQ=255) gcggcgAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGa < 1:4567061/70‑1 (MQ=255) aCGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCg > 1:3649286/1‑71 (MQ=255) ccAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTa < 1:3328664/71‑1 (MQ=255) cAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGACGAACCTACCGGTaa > 1:1291907/1‑71 (MQ=255) cAGCGTGTGGCGATTGCCCGCGCGCTGGcggtgaagccgaaaatgatgctgtttgatgaaccgacttccgc < 1:402484‑M1/71‑44 (MQ=255) aGCGTGTGGCGATTGCCCGCGCGCTGGcggtgaagccgaaaatgatgctgttt < 1:309163‑M1/53‑27 (MQ=255) gCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTAc > 1:1204247/1‑63 (MQ=255) gtgtGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTc < 1:5540739/71‑1 (MQ=255) gtgtGGCGATTGCCCGCGCGCTGGcggtgaagccgaaaa < 1:564981‑M1/39‑16 (MQ=255) tgtgGCGATTGCCCGCGCGCTGGcggtgaagccgaa < 1:1703846‑M1/36‑14 (MQ=255) tgtgGCGATTGCCCGCGCGCTGGcggtgaagccgaa < 1:1396991‑M1/36‑14 (MQ=255) tgtgGCGATTGCCCGCGCGCTGGcggtgaagccgaa < 1:82610‑M1/36‑14 (MQ=255) gtgGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCt < 1:4733480/68‑1 (MQ=255) gtgGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCt < 1:1306540/68‑1 (MQ=255) tgGCGATTGCCCGCGCGCTGGcggtgaagccgaaa < 1:5658675‑M1/35‑15 (MQ=255) tgGCGATTGCCCGCGCGCTGGcggtgaagccgaaa < 1:4063062‑M1/35‑15 (MQ=255) gCGATTGCCCGCGCGCTGGcggtgaagccgaaaatgatgctgtttgatgaaccgactt > 1:425317‑M1/1‑19 (MQ=255) cGATTGCCCGCGCGCTGGcggtgaagccgaaaatgatgctgttt < 1:3063217‑M1/44‑27 (MQ=255) cGATTGCCCGCGCGCTGGcggtgaagccgaaaatgatgctgttt < 1:739910‑M1/44‑27 (MQ=255) ttGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGt < 1:1942317/71‑1 (MQ=255) gTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGcc > 1:5064233/1‑71 (MQ=255) gTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGcc > 1:1783349/1‑71 (MQ=255) | TCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCC > Exported/756281‑756416 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |