| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 775,502 | A→G | 44.2% | L375L (TTA→TTG) ‡ | icd → | isocitrate dehydrogenase, specific for NADP+ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 775,502 | 0 | A | G | 44.2% | 37.4 / 136.5 | 113 | L375L (TTA→TTG) ‡ | icd | isocitrate dehydrogenase, specific for NADP+ |
| Reads supporting (aligned to +/- strand): ref base A (21/42); new base G (26/24); total (47/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.57e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01 | |||||||||||
Only 100 of 116 total aligned reads displayed.
GCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGAT > Exported/775438‑775571 | gCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTg < 1:2855008/69‑1 (MQ=255) tctATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTa > 1:5074671/1‑70 (MQ=255) ctATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGtt < 1:4630290/68‑1 (MQ=255) tattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAg > 1:4560182/1‑71 (MQ=255) attattCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTaa > 1:4121525/1‑61 (MQ=255) ttattCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAAt < 1:4349474/61‑1 (MQ=255) ttattCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAgg < 1:4172312/70‑1 (MQ=255) ttCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTAt < 1:1629049/70‑1 (MQ=255) tcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTAt > 1:4111056/1‑65 (MQ=255) ccGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGtt > 1:2983368/1‑56 (MQ=255) cGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAgcc < 1:5646367/70‑3 (MQ=255) cGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGgcg > 1:2200157/1‑71 (MQ=255) cGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGgcg > 1:1284159/1‑71 (MQ=255) cGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGgcg > 1:1289415/1‑71 (MQ=255) gagaTGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAg > 1:1381896/1‑64 (MQ=255) agaTGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATc < 1:1025373/71‑1 (MQ=255) gatgatGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATgg < 1:5351897/59‑1 (MQ=255) gatgatGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCa < 1:327385/71‑1 (MQ=255) ctaatgCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaa < 1:4250170/68‑1 (MQ=255) gatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAAcg > 1:1165095/1‑71 (MQ=255) gatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAAcg > 1:94836/1‑71 (MQ=255) aatgCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatg > 1:454705/2‑69 (MQ=255) aatgCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatg > 1:3045760/2‑69 (MQ=255) aatgCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatg > 1:2445968/2‑69 (MQ=255) atgCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgc > 1:1574736/1‑71 (MQ=255) tgctgcGCCATATGGGTTTGACTTAAGCGGCTGACCTGATTGTTAAAgg < 1:4330108/49‑1 (MQ=255) tgctgcGCCATATGGGTTGGTCTGAAGCGGCTGACCTGATTGTTAAAgg < 1:1080114/49‑1 (MQ=255) tgctgcGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAgg < 1:3782503/49‑1 (MQ=255) gctgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTa < 1:5342534/50‑1 (MQ=255) ctgcGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgcca < 1:989573/70‑3 (MQ=255) ctgcGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaa < 1:1203667/71‑1 (MQ=255) ctgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGaa < 1:4835775/71‑1 (MQ=255) ctgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGa < 1:312725/70‑1 (MQ=255) cgcCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaag < 1:239143/69‑2 (MQ=255) gcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAAcc < 1:5537158/71‑1 (MQ=255) ccACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAgg < 1:2080807/42‑1 (MQ=255) ccACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCg < 1:5590726/71‑1 (MQ=255) ataTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgcc < 1:1050245/60‑2 (MQ=255) ataTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgcc < 1:3276543/60‑2 (MQ=255) acaTGGGTTGGACCGAAGCGGCTGACTTAATTGTTTAAGGTa < 1:2474800/42‑1 (MQ=255) taTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgcc < 1:285809/60‑2 (MQ=255) taTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgcc < 1:3320052/60‑2 (MQ=255) caTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAgg < 1:1570199/39‑1 (MQ=255) caTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAgg < 1:320135/39‑1 (MQ=255) caTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATgg < 1:2398603/44‑1 (MQ=255) caTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTaa > 1:487027/1‑71 (MQ=255) aTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGaa > 1:53155/1‑45 (MQ=38) aTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCaa < 1:3990670/52‑1 (MQ=255) tGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa > 1:1548558/1‑35 (MQ=25) tGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTa > 1:5357055/1‑39 (MQ=37) tGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTa > 1:2179354/1‑39 (MQ=37) tGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTa > 1:96939/1‑39 (MQ=37) tGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcgc > 1:1761179/1‑49 (MQ=38) tGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCa > 1:1300947/1‑54 (MQ=38) tGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCa > 1:1562587/1‑54 (MQ=38) tGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGAcc > 1:4222670/1‑65 (MQ=255) gggTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAct > 1:2477880/1‑69 (MQ=255) gggTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAAcc > 1:3917995/1‑70 (MQ=255) ggTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAActt < 1:4593568/70‑3 (MQ=255) ggTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCt < 1:1793318/70‑1 (MQ=255) gTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAt < 1:1446041/71‑1 (MQ=255) gTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTAt < 1:2038393/71‑1 (MQ=255) ttGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATg < 1:344596/71‑1 (MQ=255) ggACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGa > 1:4061990/1‑37 (MQ=25) ggACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATc > 1:5673379/1‑47 (MQ=37) ggACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaaga > 1:1993168/1‑55 (MQ=255) ggACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGAc > 1:4050037/1‑71 (MQ=255) gACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACt < 1:561481/71‑1 (MQ=255) gACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACt < 1:3039478/71‑1 (MQ=255) aCCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGgcgc < 1:3058461/41‑1 (MQ=255) aCCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATc < 1:791951/45‑1 (MQ=255) aCCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATc < 1:2646944/45‑1 (MQ=255) ccGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAg > 1:2111704/1‑36 (MQ=255) ccGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTc < 1:1391276/71‑1 (MQ=255) tgAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTc > 1:5559034/2‑70 (MQ=255) aaGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAg > 1:3040542/1‑71 (MQ=255) aaGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAg > 1:5344229/1‑71 (MQ=255) aaGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaa > 1:2112274/1‑43 (MQ=38) aaGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCg < 1:334058/69‑1 (MQ=255) aaGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCg < 1:4433530/69‑1 (MQ=255) aaGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCg < 1:2036179/69‑1 (MQ=255) aGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGc < 1:1003337/71‑1 (MQ=255) aGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGaac > 1:3739297/1‑69 (MQ=255) gCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACg > 1:76223/1‑71 (MQ=255) ggCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTAt < 1:1292216/58‑1 (MQ=255) ggCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGt < 1:4635065/51‑1 (MQ=25) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCt < 1:909384/55‑1 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCt < 1:3635917/71‑1 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCt < 1:3725303/71‑1 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCt < 1:3995916/71‑1 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCt < 1:5212003/71‑1 (MQ=255) gCTGACCTGATTGTTAAAGGTATGGAAGGCGCAAt < 1:2608550/35‑1 (MQ=37) gCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCt > 1:1036956/1‑71 (MQ=255) cTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGt < 1:5204902/49‑1 (MQ=25) aCTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatg > 1:4622093/1‑71 (MQ=255) aCTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatg > 1:3232674/1‑71 (MQ=255) ttAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTAt < 1:5277416/51‑1 (MQ=255) tAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCg < 1:3817347/57‑1 (MQ=255) aaTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatggat < 1:4781315/70‑1 (MQ=255) aaTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatggat < 1:5175587/70‑1 (MQ=255) | GCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGAT > Exported/775438‑775571 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |