| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 775,571 | T→A | 43.1% | D398E (GAT→GAA) | icd → | isocitrate dehydrogenase, specific for NADP+ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 775,571 | 0 | T | A | 43.1% | 58.7 / 180.9 | 137 | D398E (GAT→GAA) | icd | isocitrate dehydrogenase, specific for NADP+ |
| Reads supporting (aligned to +/- strand): ref base T (31/47); new base A (21/38); total (52/85) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01 | |||||||||||
Only 100 of 165 total aligned reads displayed.
AATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCG > Exported/775502‑775633 | aaTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatggat < 1:4781315/70‑1 (MQ=255)aaTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatggat < 1:5175587/70‑1 (MQ=255) aTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatggatg < 1:311700/70‑1 (MQ=255) aaaGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTa < 1:536297/70‑1 (MQ=255) aaGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTaag > 1:5649732/1‑70 (MQ=255) aaGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa < 1:2884133/71‑1 (MQ=255) ggTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGGCTGATGGATGGCGCTAAAct < 1:3408985/71‑1 (MQ=255) aTGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAgctgct > 1:1860602/1‑71 (MQ=255) gAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGa > 1:1651041/1‑70 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaa < 1:3147756/71‑1 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa > 1:2422665/1‑70 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGCATGGCGCTAAACTGCTGaa < 1:1418655/71‑1 (MQ=255) cgcAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGtt < 1:379986/71‑1 (MQ=255) cAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:2774159/1‑56 (MQ=255) cAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGtt > 1:2122522/1‑69 (MQ=255) cAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTATGATGGATGGCGCTAAACTTCTGAAATGTTCa < 1:1612606/71‑1 (MQ=255) aTCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGatggatgg > 1:1237810/1‑47 (MQ=255) tCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGgcg < 1:3514670/48‑1 (MQ=16) tCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGtt < 1:2951441/66‑1 (MQ=255) tCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCagag > 1:437349/1‑71 (MQ=255) tCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCagag > 1:1999120/1‑71 (MQ=255) aaCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTa < 1:3365003/49‑1 (MQ=255) aaCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAgtgt < 1:1036445/70‑1 (MQ=255) aCGGGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa < 1:112212/50‑1 (MQ=255) aCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa < 1:1823507/50‑1 (MQ=255) aCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGttt < 1:2189580/71‑1 (MQ=255) cgAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt < 1:2182948/71‑1 (MQ=255) aagaCCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTg > 1:1538154/4‑70 (MQ=255) aagaCCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGa < 1:3293415/68‑1 (MQ=255) aagaCCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGa < 1:3300178/68‑1 (MQ=255) agaCCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGa > 1:3718769/3‑51 (MQ=255) agaCCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGa > 1:640260/3‑51 (MQ=255) aaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaaa > 1:3692746/1‑52 (MQ=255) ccGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAagcg < 1:917785/71‑1 (MQ=255) ccGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcgc > 1:4789614/1‑70 (MQ=255) cGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGa > 1:4844237/1‑66 (MQ=255) cGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGa > 1:41491/1‑66 (MQ=255) gTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGat < 1:2866702/71‑1 (MQ=255) gTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTg > 1:1147991/1‑64 (MQ=255) gTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGat < 1:5216023/71‑1 (MQ=255) gTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGat < 1:808205/71‑1 (MQ=255) tAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatc < 1:4950922/71‑1 (MQ=255) tAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGat > 1:4107747/1‑70 (MQ=255) aaCTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGatca > 1:4187107/1‑71 (MQ=255) acttATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGatcat > 1:4867709/4‑71 (MQ=255) ttATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAagcg < 1:4209301/63‑1 (MQ=255) tATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCg > 1:716730/1‑70 (MQ=255) aTGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGa > 1:4506782/1‑70 (MQ=255) aTGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGa > 1:4982221/1‑70 (MQ=255) aTGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGa < 1:612903/70‑1 (MQ=255) aTGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGa > 1:4938828/1‑70 (MQ=255) tGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGaa < 1:5304580/70‑1 (MQ=255) tGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGaa < 1:3684003/70‑1 (MQ=255) aCTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGa > 1:1053756/1‑55 (MQ=255) cTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt < 1:4529868/52‑1 (MQ=255) cTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt < 1:5495997/52‑1 (MQ=255) ttCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGttt < 1:3940177/48‑1 (MQ=255) ttCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGttt < 1:3593329/48‑1 (MQ=255) ttCGAACGTCTGATGGAAGGCTCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGaaaa < 1:838483/68‑1 (MQ=255) ttCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGaaaa < 1:5492544/68‑1 (MQ=255) ttCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGaaaa < 1:4308074/68‑1 (MQ=255) tCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGa > 1:391449/1‑52 (MQ=255) tCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcgcg < 1:2784171/56‑1 (MQ=255) tCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGttt < 1:2511959/47‑1 (MQ=38) tCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGttt < 1:2690855/47‑1 (MQ=38) tCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGtt < 1:4886163/46‑1 (MQ=38) tCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGtt < 1:176410/46‑1 (MQ=38) tCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGtt < 1:1550097/46‑1 (MQ=38) tCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGtt < 1:3824699/46‑1 (MQ=38) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaa > 1:2665221/1‑65 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATg > 1:2732202/1‑70 (MQ=255) cGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGa > 1:5459182/1‑51 (MQ=255) cGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGa < 1:5070083/63‑1 (MQ=255) cGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACa > 1:5101980/1‑68 (MQ=255) gaacGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGt < 1:2152004/67‑1 (MQ=255) gaacGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGt < 1:821401/67‑1 (MQ=255) gaacGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGt < 1:3918859/67‑1 (MQ=255) gaacGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTa < 1:3284446/68‑1 (MQ=255) gAGCGTCTGATGGATGGCGCTAAACTCCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt < 1:5111052/70‑1 (MQ=255) tCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGtt < 1:4693766/39‑1 (MQ=255) tCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcgcg < 1:3225806/49‑1 (MQ=255) tCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaa < 1:84957/58‑1 (MQ=255) tCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATg < 1:3378872/64‑1 (MQ=255) tGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaa < 1:539490/56‑1 (MQ=255) tGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCg > 1:2912800/1‑70 (MQ=255) tGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGTTGAAGCGatca < 1:453510/51‑1 (MQ=255) gatggatgGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCg > 1:2096488/1‑69 (MQ=255) gatggaagGCGCTAAGCTGCTTAAATGTTCAGAGTTTGGTGaa < 1:2282279/43‑2 (MQ=37) gatggaagGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGaa < 1:3245968/43‑2 (MQ=38) atggatggCGCTAAACTGCTGAAATGTTCAGAGtt > 1:3421879/1‑35 (MQ=255) atggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt < 1:1355639/61‑1 (MQ=255) atggaaggCGCTAAGCTGCTGAAATGTTCAGAGTTTg > 1:2153878/1‑37 (MQ=37) tggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGt > 1:3423067/1‑38 (MQ=255) tggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGt > 1:3095976/1‑38 (MQ=255) tggaaggCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAagc > 1:2191942/1‑43 (MQ=37) gatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGt < 1:4131663/36‑1 (MQ=255) gatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGt < 1:841309/36‑1 (MQ=255) gatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaaa > 1:349245/1‑53 (MQ=255) gatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACAt > 1:3560578/1‑56 (MQ=255) gatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACAt > 1:4335646/1‑56 (MQ=255) | AATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCG > Exported/775502‑775633 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |