BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	821,445	T→C	75.0%	S7G (AGC→GGC)	ldrA ←	toxic polypeptide, small

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	821,445	0	T	C	75.0%	67.3 / 22.4	44	S7G (AGC→GGC)	ldrA	toxic polypeptide, small
Reads supporting (aligned to +/- strand): ref base T (2/9); new base C (18/15); total (20/24)
Fisher's exact test for biased strand distribution p-value = 4.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTA  >  Exported/821395‑821506

cGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGt                                           >  1:1416822/1‑71 (MQ=255)

cGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGt                                           >  1:4303630/1‑71 (MQ=255)

 gCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTc                                          <  1:4339373/71‑1 (MQ=255)

 gCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTc                                          <  1:1710798/71‑1 (MQ=255)

 gCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTc                                          <  1:2501277/71‑1 (MQ=255)

  ccaccaGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCa                                         <  1:3524084/71‑1 (MQ=34)

   caccaGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCAt                                        >  1:1216590/1‑71 (MQ=255)

    accaGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATg                                       >  1:4371520/1‑71 (MQ=255)

     ccaGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATgg                                      >  1:5172359/1‑71 (MQ=34)

       aGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCa                                    <  1:3448904/71‑1 (MQ=34)

       aGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGc                                     >  1:647017/1‑70 (MQ=255)

       aGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCa                                    <  1:4313762/71‑1 (MQ=255)

         cTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCaa                                   <  1:1627951/70‑1 (MQ=35)

                 cGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTgcgc                            >  1:241570/1‑69 (MQ=25)

                  gCTGCGGTAATAATTCCCGCCAGGATCGTTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAg                         >  1:2195765/1‑71 (MQ=255)

                  gCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCa                                         >  1:2356212/1‑55 (MQ=35)

                  gCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGa                          <  1:2454213/70‑1 (MQ=25)

                     gCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACt                                >  1:5638646/1‑61 (MQ=25)

                      cGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTc                     <  1:4204334/71‑1 (MQ=34)

                      cGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCg                       <  1:3076164/69‑1 (MQ=255)

                       ggTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCa                    <  1:4023875/71‑1 (MQ=255)

                        gTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCat                   <  1:5586855/71‑1 (MQ=255)

                         taataaTTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCata                  <  1:1851649/71‑1 (MQ=255)

                          aataatTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCata                  >  1:2923647/1‑70 (MQ=255)

                          aataatTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCata                  >  1:2321353/1‑70 (MQ=255)

                           ataatTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCata                  >  1:4554846/1‑69 (MQ=255)

                            taatTCCCGCCAGGATCGGTGCTGCCAGTTCGTGCCAGAAAGTCATGGCAAACTgc                              <  1:149218/56‑1 (MQ=255)

                            taatTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACt                                <  1:2683247/54‑1 (MQ=35)

                            taatTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTgc                              <  1:5047611/56‑1 (MQ=35)

                            taatTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTgcgc                            <  1:1263092/58‑1 (MQ=255)

                            taatTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAg               >  1:55659/1‑71 (MQ=21)

                               ttCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATg                                       <  1:1596578/44‑1 (MQ=255)

                               ttCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCaaa                                  >  1:4809753/1‑49 (MQ=255)

                                 cccGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTc                                          >  1:5463922/1‑39 (MQ=255)

                                 cccGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTc                                          >  1:1615482/1‑39 (MQ=255)

                                 cccGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTc                                          >  1:4940680/1‑39 (MQ=255)

                                  ccGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCaaa                                  >  1:1809766/1‑46 (MQ=255)

                                    gCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCttg       <  1:3886981/71‑1 (MQ=34)

                                    gCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCttg       <  1:2042218/71‑1 (MQ=34)

                                      cAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGTCAAACTGCGCGAGCGTCa                    <  1:3270180/56‑1 (MQ=11)

                                      cAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCa                    <  1:4504964/56‑1 (MQ=255)

                                      cAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCa                    <  1:1160594/56‑1 (MQ=255)

                                       aGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCttgttg    >  1:5482186/1‑71 (MQ=21)

                                              gTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTa  <  1:4394382/66‑1 (MQ=25)

CGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTA  >  Exported/821395‑821506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`