| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 821,535 | G→T | 23.5% | intergenic (‑72/+382) | ldrA ← / ← ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 821,535 | 0 | G | T | 23.5% | 117.1 / 20.6 | 51 | intergenic (‑72/+382) | ldrA/ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Reads supporting (aligned to +/- strand): ref base G (16/23); new base T (4/8); total (20/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
GCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCT > Exported/821493‑821604 | gccgccTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAAATTAGCCCCCGTTc < 1:3016986/71‑1 (MQ=14) cgccTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCtg < 1:4219937/71‑1 (MQ=34) ccTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCtgtt > 1:4893763/1‑71 (MQ=34) ccTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCtgt > 1:589954/1‑70 (MQ=35) cTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCtgttg < 1:2806379/71‑1 (MQ=34) cTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCtgttg < 1:1714718/71‑1 (MQ=34) tgttgtAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCtgtt < 1:940135/68‑1 (MQ=35) gttgtAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCtgttgt > 1:374624/1‑69 (MQ=35) gtAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAgg < 1:3061370/70‑1 (MQ=35) gtAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAgg < 1:4660628/70‑1 (MQ=35) aTGACAACATTTTGCGGCTATTCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGttgt < 1:155652/71‑3 (MQ=11) aTGACAACATTTTGCGGCTATTCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGttg > 1:3273401/1‑69 (MQ=11) aTGACAACATTTTGCGGCTATTCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGttg > 1:1338530/1‑69 (MQ=11) aTGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGtttt < 1:1909009/71‑1 (MQ=34) aTGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGttt > 1:4937720/1‑70 (MQ=35) aTGACAACATTTTGCGACTATTCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGttgt < 1:3754784/71‑3 (MQ=11) tGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAg < 1:2893014/65‑1 (MQ=35) aacaTTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACctc > 1:762428/1‑71 (MQ=34) aacaTTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACctc > 1:1018866/1‑71 (MQ=34) aacaTTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACctc > 1:4271205/1‑71 (MQ=34) acaTTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACctc < 1:5348219/70‑1 (MQ=35) caTTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACctc < 1:2749988/69‑1 (MQ=35) aTTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCtctc > 1:4164091/1‑70 (MQ=35) ttGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACg > 1:1730592/1‑71 (MQ=34) cGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGt < 1:753174/69‑1 (MQ=35) gCTATTCTTGAATTG‑TTCTGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACct < 1:1570890/59‑1 (MQ=11) gCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTg < 1:1546760/68‑1 (MQ=35) gCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTg < 1:1553419/68‑1 (MQ=35) gCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTg < 1:3472529/68‑1 (MQ=35) gCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTg < 1:2987968/68‑1 (MQ=35) gCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTg < 1:4483300/68‑1 (MQ=35) cTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCggg > 1:1886804/1‑71 (MQ=34) cTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCgg < 1:175163/70‑1 (MQ=35) tATTCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGttgt < 1:3070741/53‑3 (MQ=11) ttCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCtgtt < 1:148253/41‑1 (MQ=9) ttCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAg < 1:4106768/46‑1 (MQ=11) ttCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAg < 1:281134/46‑1 (MQ=11) ttCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGt > 1:1870206/1‑71 (MQ=11) ttCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGt > 1:1290788/1‑71 (MQ=11) ttCTTGAATTG‑TTCAGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCaa < 1:1278158/60‑1 (MQ=11) tCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCa < 1:3543476/44‑1 (MQ=38) tCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGtt > 1:5693301/1‑71 (MQ=34) cTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTAc > 1:2826786/1‑51 (MQ=35) ttGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAgg > 1:1133119/1‑44 (MQ=38) ttGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAgg > 1:1922856/1‑44 (MQ=38) ttGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCATGTTTTACCTCTCAACGTGCGGGGGtt < 1:3721880/69‑1 (MQ=25) ttGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGttt < 1:1544248/47‑1 (MQ=38) ttGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGtt > 1:640946/1‑46 (MQ=38) ttGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTa < 1:3133947/49‑1 (MQ=38) ttGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGttt > 1:756040/1‑70 (MQ=35) aGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTctct < 1:459328/71‑1 (MQ=34) gtg‑tCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTAc > 1:759367/1‑45 (MQ=38) | GCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCT > Exported/821493‑821604 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |