| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 821,760 | G→A | 50.0% | intergenic (‑297/+157) | ldrA ← / ← ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 821,760 | 0 | G | A | 50.0% | 32.2 / 142.4 | 104 | intergenic (‑297/+157) | ldrA/ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Reads supporting (aligned to +/- strand): ref base G (22/30); new base A (22/30); total (44/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01 | |||||||||||
Only 100 of 107 total aligned reads displayed.
AATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGAT > Exported/821692‑821829 | aaTTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTg < 1:2788131/69‑1 (MQ=35) aTCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGt < 1:3024025/71‑1 (MQ=39) ttcacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGtt > 1:3937129/1‑69 (MQ=40) ttcacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGt < 1:4887246/68‑1 (MQ=40) ttcacttcaTCCCTGAAATGTTTGCAATAAAGAGAACATTCCGGCTTTTCAACAGCTGTTGCAGTCGtt > 1:320400/1‑69 (MQ=255) tcacttcaTCCCTGAAATGTTTGCAATAAAGAGAACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTc < 1:576134/70‑1 (MQ=255) cacttcaTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCa > 1:12353/1‑70 (MQ=11) cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGttt < 1:1446410/68‑1 (MQ=40) cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTc < 1:766275/69‑1 (MQ=40) cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTc < 1:4797940/69‑1 (MQ=40) cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTc < 1:2007512/69‑1 (MQ=40) cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCa > 1:2046808/1‑70 (MQ=255) cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCAt > 1:2184858/1‑71 (MQ=255) acttcaTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCAt < 1:5603295/70‑1 (MQ=11) acttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCAt < 1:2873495/70‑1 (MQ=255) acttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCAt < 1:523176/70‑1 (MQ=255) acttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCAt < 1:4844769/70‑1 (MQ=255) acttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATg < 1:2383303/71‑1 (MQ=255) acttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATg < 1:216736/71‑1 (MQ=255) cttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGa < 1:1444423/71‑1 (MQ=255) caTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGa > 1:1055425/1‑68 (MQ=11) aTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGc < 1:1585178/71‑1 (MQ=255) tCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCAt < 1:4156454/64‑1 (MQ=255) cccTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGct > 1:5006544/1‑70 (MQ=255) cTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTg < 1:4718052/71‑1 (MQ=17) gAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGga > 1:2975512/1‑71 (MQ=17) gAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGctc > 1:3716784/1‑67 (MQ=255) aaaTGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCAt < 1:1047272/58‑1 (MQ=11) aaaTGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgat < 1:2869499/71‑1 (MQ=17) aaaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGgat < 1:3996150/71‑1 (MQ=255) aaaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGgat < 1:3052047/71‑1 (MQ=255) aaaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGgat < 1:5206207/71‑1 (MQ=255) aaaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGga > 1:807401/1‑70 (MQ=255) aaTGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatg < 1:3749695/71‑1 (MQ=18) tttGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGc > 1:385316/1‑71 (MQ=18) ttGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatg > 1:4706826/1‑66 (MQ=18) ttGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCt > 1:1955198/1‑71 (MQ=18) ttGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGc > 1:2712986/1‑41 (MQ=38) tGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatgag > 1:4322892/1‑66 (MQ=18) tGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatgag > 1:5250076/1‑66 (MQ=18) tGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCt < 1:2690654/70‑1 (MQ=18) tGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTg > 1:4844364/1‑56 (MQ=255) aaTGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTg < 1:1423380/58‑1 (MQ=17) aaTGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTg < 1:4827439/58‑1 (MQ=17) aTAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGc < 1:4906049/36‑1 (MQ=40) tgaaGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATg < 1:4325493/45‑1 (MQ=14) tgaaGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATg < 1:1688084/45‑1 (MQ=14) aaaGAGTACATTCCGGCTTTTCAACAGCTGTTGCAg > 1:2203093/1‑36 (MQ=33) aaGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatga > 1:504803/1‑59 (MQ=21) aaGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTc < 1:5614866/71‑1 (MQ=25) gagTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCgg > 1:3947259/1‑71 (MQ=37) gagTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCgg < 1:329877/71‑1 (MQ=37) gagTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCgg < 1:3616340/71‑1 (MQ=37) gagTGCATTCCGG‑TTTTTAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCggg > 1:1143634/1‑71 (MQ=255) agTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCa < 1:4530933/40‑1 (MQ=255) agTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGgatga < 1:2635013/56‑1 (MQ=255) gtgcATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGt < 1:3826935/68‑1 (MQ=37) tgcATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatga > 1:2663971/3‑54 (MQ=25) tACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGt > 1:1192865/1‑43 (MQ=255) tACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGatgatg > 1:4154224/1‑56 (MQ=255) aCATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTg > 1:4311177/1‑71 (MQ=255) cATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCAt > 1:5547527/1‑37 (MQ=17) cATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGa > 1:1312919/1‑39 (MQ=17) aTTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGcc < 1:501107/71‑1 (MQ=38) ttCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAg < 1:1082392/59‑1 (MQ=37) ttCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAg < 1:545143/59‑1 (MQ=37) ccGGTTTTTCAACAGCTGTTACAGTCATTTCATGa > 1:650708/1‑35 (MQ=18) ccGGTTTTTCAACAGCTGTTACAGTCATTTCATGa > 1:2092500/1‑35 (MQ=18) ccGGTTTTTCAACAGCTGTTACAGTCATTTCATGa > 1:226352/1‑35 (MQ=18) ccGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCaa < 1:2800549/70‑1 (MQ=255) ccGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCaa < 1:5418685/70‑1 (MQ=255) cGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGga > 1:1791373/1‑44 (MQ=25) tttttCAACAGCTGTTACAGTCATTTCATGAGTGctc < 1:21836/36‑1 (MQ=25) tttttCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGt > 1:1653836/2‑60 (MQ=255) tttttCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAat < 1:2084324/66‑1 (MQ=255) cTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGc > 1:2022357/1‑47 (MQ=255) cTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGc > 1:1070892/1‑47 (MQ=255) cTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATAttt < 1:4230168/71‑1 (MQ=255) cTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATAttt < 1:5015339/71‑1 (MQ=255) ttttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCtt < 1:4371243/48‑1 (MQ=255) tttCAACAGCTGTTACAGTCATTTCATGAGTGctc < 1:1060769/35‑1 (MQ=25) tttCAACAGCTGTTACAGTCATTTCATGAGTGctc < 1:1971190/35‑1 (MQ=25) tttCAACAGCTGTTACAGTCATTTCATGAGTGctc < 1:1166742/35‑1 (MQ=25) tttCAACAGCTGTTACAGTCATTTCATGAGTGctc < 1:1424914/35‑1 (MQ=25) tttCAACAGCTGTTACAGTCATTTCATGAGTGctc < 1:1963204/35‑1 (MQ=25) tttCAACAGCTGTTACAGTCATTTCATGAGTGctc < 1:203470/35‑1 (MQ=25) tttCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAata > 1:4012282/1‑66 (MQ=255) ttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTcc > 1:1946119/1‑48 (MQ=255) ttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTcc > 1:3830683/1‑48 (MQ=255) ttCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTAc < 1:412948/70‑1 (MQ=255) tCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTcc > 1:2781536/1‑47 (MQ=255) tCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTcc > 1:723886/1‑47 (MQ=255) tCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCaa > 1:5660595/1‑62 (MQ=255) tCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTa < 1:3713959/68‑1 (MQ=255) tCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTACCAGCTCGGGTTGCCa > 1:2337431/1‑61 (MQ=255) cAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTgg < 1:2585273/71‑1 (MQ=255) tGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGaa > 1:921513/1‑69 (MQ=255) ttGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCa < 1:5501011/51‑1 (MQ=255) gCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGAt < 1:1084642/70‑1 (MQ=255) gCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGAt < 1:3781259/70‑1 (MQ=255) | AATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGAT > Exported/821692‑821829 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |