Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 821,788 T→G 45.5% intergenic (‑325/+129) ldrA ← / ← ldrC toxic polypeptide, small/toxic polypeptide, small

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported821,7880TG45.5% ‑6.2 / 77.6 55intergenic (‑325/+129)ldrA/ldrCtoxic polypeptide, small/toxic polypeptide, small
Reads supporting (aligned to +/- strand):  ref base T (13/17);  new base G (10/15);  total (23/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

TTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGT  >  Exported/821720‑821854
                                                                    |                                                                  
tttGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGc                                                                  >  1:385316/1‑71 (MQ=18)
 ttGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCt                                                                 >  1:1955198/1‑71 (MQ=18)
  tGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCt                                                                 <  1:2690654/70‑1 (MQ=18)
         aaGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTc                                                         <  1:5614866/71‑1 (MQ=25)
           gagTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCgg                                                       <  1:3616340/71‑1 (MQ=37)
           gagTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCgg                                                       <  1:329877/71‑1 (MQ=37)
           gagTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCgg                                                       >  1:3947259/1‑71 (MQ=37)
           gagTGCATTCCGGTTTTTAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCggg                                                      >  1:1143634/1‑71 (MQ=255)
             gtgcATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGt                                                     <  1:3826935/68‑1 (MQ=37)
              tACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGatgatg                                                                   >  1:4154224/1‑56 (MQ=255)
               aCATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTg                                                   >  1:4311177/1‑71 (MQ=255)
                 aTTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGcc                                                 <  1:501107/71‑1 (MQ=38)
                  ttCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAg                                                            <  1:1082392/59‑1 (MQ=37)
                  ttCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAg                                                            <  1:545143/59‑1 (MQ=37)
                    ccGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCaa                                               <  1:2800549/70‑1 (MQ=255)
                    ccGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCaa                                               <  1:5418685/70‑1 (MQ=255)
                     cGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCgg                                                       <  1:2158097/61‑1 (MQ=255)
                        tttttCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGt                                                     >  1:1653836/2‑60 (MQ=255)
                        tttttCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAat                                              <  1:2084324/66‑1 (MQ=255)
                        cTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGc                                                                  >  1:2022357/1‑47 (MQ=255)
                        cTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGc                                                                  >  1:1070892/1‑47 (MQ=255)
                        cTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATAttt                                          <  1:4230168/71‑1 (MQ=255)
                        cTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATAttt                                          <  1:5015339/71‑1 (MQ=255)
                         ttttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCtt                                                                <  1:4371243/48‑1 (MQ=255)
                          tttCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAata                                             >  1:4012282/1‑66 (MQ=255)
                           ttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTcc                                                              >  1:3830683/1‑48 (MQ=255)
                           ttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTcc                                                              >  1:1946119/1‑48 (MQ=255)
                           ttCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTAc                                        <  1:412948/70‑1 (MQ=255)
                            tCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTcc                                                              >  1:2781536/1‑47 (MQ=255)
                            tCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTcc                                                              >  1:723886/1‑47 (MQ=255)
                            tCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCaa                                               >  1:5660595/1‑62 (MQ=255)
                            tCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTa                                         <  1:3713959/68‑1 (MQ=255)
                            tCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTACCAGCTCGGGTTGCCa                                                >  1:2337431/1‑61 (MQ=255)
                                cAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTgg                                  <  1:2585273/71‑1 (MQ=255)
                                    tGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGaa                                >  1:921513/1‑69 (MQ=255)
                                      ttGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCa                                                <  1:5501011/51‑1 (MQ=255)
                                        gCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGAt                           <  1:1084642/70‑1 (MQ=255)
                                        gCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGAt                           <  1:3781259/70‑1 (MQ=255)
                                        acAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGaa                                >  1:1986682/2‑65 (MQ=255)
                                         cAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATa                          <  1:2591327/70‑1 (MQ=255)
                                         cAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATa                          <  1:5597983/70‑1 (MQ=255)
                                            tCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTa                                         <  1:1360273/52‑1 (MQ=255)
                                              gTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTAc                                        >  1:1739819/1‑51 (MQ=255)
                                              gTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTAc                                        >  1:4856740/1‑51 (MQ=255)
                                              gTTTCATGAGTGCTCTGGATGATGCTTCCAGCTAGGGTTGCCAATATTTAc                                        >  1:1330868/1‑51 (MQ=255)
                                                 tCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATAtt                                           >  1:4039707/1‑45 (MQ=255)
                                                 tCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACaaa                  <  1:2494345/70‑1 (MQ=255)
                                                   aTGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACaaaaa                <  1:4944308/70‑1 (MQ=255)
                                                          ctctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa        <  1:1054012/71‑1 (MQ=255)
                                                          ctctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa        <  1:1508998/71‑1 (MQ=255)
                                                           tctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGaa                                <  1:2090510/46‑1 (MQ=255)
                                                           tctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGaa                                <  1:5363010/46‑1 (MQ=255)
                                                            ctGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACaaa                  <  1:1141625/59‑1 (MQ=255)
                                                             tGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGAc                     >  1:34747/1‑55 (MQ=255)
                                                             tGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa        <  1:2228891/68‑1 (MQ=255)
                                                              ggATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGc     >  1:2077133/1‑70 (MQ=255)
                                                               gatgatGCTTCCAGCTCGGGTTGCCAATATTTACtt                                      <  1:3632523/36‑1 (MQ=255)
                                                               gatgatGCTTCCAGCTCGGGTTGCCAATATTTACtt                                      <  1:4916776/36‑1 (MQ=255)
                                                               gatgatGCTTCCAGCTCGGGTTGCCAATATTTACtt                                      <  1:1575054/36‑1 (MQ=255)
                                                               gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa        >  1:4257911/1‑66 (MQ=255)
                                                               gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAgg      <  1:2680119/68‑1 (MQ=255)
                                                               gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAgg      <  1:4582793/68‑1 (MQ=255)
                                                               gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGc     <  1:3805761/69‑1 (MQ=255)
                                                               gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCt    <  1:3346073/70‑1 (MQ=255)
                                                               gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTg   >  1:2476154/1‑71 (MQ=255)
                                                               gatgagGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCt    <  1:2602855/70‑1 (MQ=255)
                                                                atgaggCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGt  <  1:354236/71‑1 (MQ=255)
                                                                    tgCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGc            <  1:4883770/57‑1 (MQ=255)
                                                                    |                                                                  
TTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGT  >  Exported/821720‑821854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: