Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 821,788 | T→G | 45.5% | intergenic (‑325/+129) | ldrA ← / ← ldrC | toxic polypeptide, small/toxic polypeptide, small |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 821,788 | 0 | T | G | 45.5% | ‑6.2 / 77.6 | 55 | intergenic (‑325/+129) | ldrA/ldrC | toxic polypeptide, small/toxic polypeptide, small |
Reads supporting (aligned to +/- strand): ref base T (13/17); new base G (10/15); total (23/32) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 |
TTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGT > Exported/821720‑821854 | tttGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGc > 1:385316/1‑71 (MQ=18) ttGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCt > 1:1955198/1‑71 (MQ=18) tGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCt < 1:2690654/70‑1 (MQ=18) aaGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTc < 1:5614866/71‑1 (MQ=25) gagTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCgg < 1:3616340/71‑1 (MQ=37) gagTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCgg < 1:329877/71‑1 (MQ=37) gagTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCgg > 1:3947259/1‑71 (MQ=37) gagTGCATTCCGG‑TTTTTAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCggg > 1:1143634/1‑71 (MQ=255) gtgcATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGt < 1:3826935/68‑1 (MQ=37) tACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGatgatg > 1:4154224/1‑56 (MQ=255) aCATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTg > 1:4311177/1‑71 (MQ=255) aTTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGcc < 1:501107/71‑1 (MQ=38) ttCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAg < 1:1082392/59‑1 (MQ=37) ttCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAg < 1:545143/59‑1 (MQ=37) ccGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCaa < 1:2800549/70‑1 (MQ=255) ccGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCaa < 1:5418685/70‑1 (MQ=255) cGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCgg < 1:2158097/61‑1 (MQ=255) tttttCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGt > 1:1653836/2‑60 (MQ=255) tttttCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAat < 1:2084324/66‑1 (MQ=255) cTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGc > 1:2022357/1‑47 (MQ=255) cTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGc > 1:1070892/1‑47 (MQ=255) cTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATAttt < 1:4230168/71‑1 (MQ=255) cTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATAttt < 1:5015339/71‑1 (MQ=255) ttttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCtt < 1:4371243/48‑1 (MQ=255) tttCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAata > 1:4012282/1‑66 (MQ=255) ttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTcc > 1:3830683/1‑48 (MQ=255) ttCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTcc > 1:1946119/1‑48 (MQ=255) ttCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTAc < 1:412948/70‑1 (MQ=255) tCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTcc > 1:2781536/1‑47 (MQ=255) tCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTcc > 1:723886/1‑47 (MQ=255) tCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCaa > 1:5660595/1‑62 (MQ=255) tCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTa < 1:3713959/68‑1 (MQ=255) tCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTACCAGCTCGGGTTGCCa > 1:2337431/1‑61 (MQ=255) cAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTgg < 1:2585273/71‑1 (MQ=255) tGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGaa > 1:921513/1‑69 (MQ=255) ttGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCa < 1:5501011/51‑1 (MQ=255) gCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGAt < 1:1084642/70‑1 (MQ=255) gCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGAt < 1:3781259/70‑1 (MQ=255) acAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGaa > 1:1986682/2‑65 (MQ=255) cAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATa < 1:2591327/70‑1 (MQ=255) cAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATa < 1:5597983/70‑1 (MQ=255) tCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTa < 1:1360273/52‑1 (MQ=255) gTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTAc > 1:1739819/1‑51 (MQ=255) gTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTAc > 1:4856740/1‑51 (MQ=255) gTTTCATGAGTGCTCTGGATGATGCTTCCAGCTAGGGTTGCCAATATTTAc > 1:1330868/1‑51 (MQ=255) tCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATAtt > 1:4039707/1‑45 (MQ=255) tCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACaaa < 1:2494345/70‑1 (MQ=255) aTGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACaaaaa < 1:4944308/70‑1 (MQ=255) ctctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa < 1:1054012/71‑1 (MQ=255) ctctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa < 1:1508998/71‑1 (MQ=255) tctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGaa < 1:2090510/46‑1 (MQ=255) tctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGaa < 1:5363010/46‑1 (MQ=255) ctGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACaaa < 1:1141625/59‑1 (MQ=255) tGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGAc > 1:34747/1‑55 (MQ=255) tGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa < 1:2228891/68‑1 (MQ=255) ggATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGc > 1:2077133/1‑70 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACtt < 1:3632523/36‑1 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACtt < 1:4916776/36‑1 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACtt < 1:1575054/36‑1 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa > 1:4257911/1‑66 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAgg < 1:2680119/68‑1 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAgg < 1:4582793/68‑1 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGc < 1:3805761/69‑1 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCt < 1:3346073/70‑1 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTg > 1:2476154/1‑71 (MQ=255) gatgagGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCt < 1:2602855/70‑1 (MQ=255) atgaggCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGt < 1:354236/71‑1 (MQ=255) tgCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGc < 1:4883770/57‑1 (MQ=255) | TTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGT > Exported/821720‑821854 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |