Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 832,181 T→C 16.4% T213T (ACT→ACC narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported832,1810TC16.4% 243.3 / 34.7 106T213T (ACT→ACCnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (44/44);  new base C (5/12);  total (49/57)
Fisher's exact test for biased strand distribution p-value = 1.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 112 total aligned reads displayed.

ATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACT  >  Exported/832116‑832250
                                                                 |                                                                     
atcccGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGttt                                                                  <  1:921405/68‑1 (MQ=255)
atcccGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGttt                                                                  <  1:3742798/68‑1 (MQ=255)
aTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACt                                                                       <  1:927527/66‑1 (MQ=255)
aTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                                  <  1:3642011/71‑1 (MQ=255)
aTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                                  <  1:4752251/71‑1 (MQ=255)
aTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCt                                                                   >  1:4678871/1‑70 (MQ=255)
aTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCt                                                                   >  1:4341106/1‑70 (MQ=255)
   ccGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAg                                                               >  1:592971/1‑71 (MQ=255)
   ccGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAg                                                               >  1:2197797/1‑71 (MQ=255)
    cGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTa                                                                 <  1:1759196/68‑1 (MQ=255)
    cGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTa                                                                 <  1:1411874/68‑1 (MQ=255)
     ggCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCt                                                             <  1:5232913/71‑1 (MQ=255)
      gCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCtt                                                            >  1:1360984/1‑71 (MQ=255)
      gCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCtt                                                            >  1:1552504/1‑71 (MQ=255)
      gCAATGTCGATGGTTTCTTACGCATCGGGTGAACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCt                                                             >  1:3361782/1‑70 (MQ=255)
       cAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGTCGGTACTTGCTTAAGCTTc                                                           <  1:5181192/71‑1 (MQ=255)
       cAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                                           <  1:1379888/71‑1 (MQ=255)
         aTGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACt                                                                       >  1:2705791/1‑57 (MQ=255)
         aTGTCGATGGTTTCTTACGCATCGGGTGAACGCTATCTCTCGCTGATTGGCGGTACt                                                                       >  1:1341708/1‑57 (MQ=255)
          tGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTAc                                                        <  1:4317805/71‑1 (MQ=255)
          tGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTAc                                                        >  1:1451138/1‑71 (MQ=255)
            tCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTa                                                                 <  1:1159936/60‑1 (MQ=255)
            tCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTa                                                                 <  1:145463/60‑1 (MQ=255)
            tCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGa                                                      >  1:4296815/1‑71 (MQ=255)
              gATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACt                                                    >  1:2149607/1‑71 (MQ=255)
              gATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACt                                                    >  1:2666366/1‑71 (MQ=255)
              gATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACt                                                    >  1:2233995/1‑71 (MQ=255)
                tGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTgg                                                  <  1:2098656/71‑1 (MQ=255)
                 ggTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTgg                                                  >  1:4249428/1‑70 (MQ=255)
                  gTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGt                                                 <  1:5306882/70‑1 (MQ=255)
                  gTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                                                >  1:1816686/1‑71 (MQ=255)
                  gTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                                                >  1:1920521/1‑71 (MQ=255)
                   tttCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTAc                                               >  1:586300/1‑71 (MQ=255)
                    ttCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACt                                                    <  1:1504544/65‑1 (MQ=255)
                    ttCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                                  <  1:828072/51‑1 (MQ=255)
                    ttCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                                  <  1:636632/51‑1 (MQ=255)
                     tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACt                                                    >  1:5542322/1‑64 (MQ=255)
                     tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACt                                              <  1:4581840/70‑1 (MQ=255)
                     tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACt                                              <  1:428408/70‑1 (MQ=255)
                     tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTg                                             >  1:3482011/1‑71 (MQ=255)
                     tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTg                                             >  1:3910588/1‑71 (MQ=255)
                       ttACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                                           >  1:291284/1‑55 (MQ=255)
                         aCGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                                  >  1:4700812/1‑46 (MQ=255)
                         aCGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                                  >  1:4341842/1‑46 (MQ=255)
                         aCGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGc                                            <  1:4645435/68‑1 (MQ=255)
                          cGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTAc                                                        <  1:5182964/55‑1 (MQ=255)
                          cGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACt                                        <  1:4281585/71‑1 (MQ=255)
                           gcttCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGc                                            <  1:798671/63‑1 (MQ=255)
                           gCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                       <  1:3784066/71‑1 (MQ=255)
                             aTCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGAc                                         >  1:2123407/1‑67 (MQ=255)
                             aTCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGAc                                         >  1:3373802/1‑67 (MQ=255)
                              tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                                  <  1:38016/41‑1 (MQ=255)
                              tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                                  <  1:4734857/41‑1 (MQ=255)
                              tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                                  <  1:2032844/41‑1 (MQ=255)
                              tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcc                                    <  1:121244/71‑1 (MQ=255)
                              tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcc                                    <  1:2835482/71‑1 (MQ=255)
                               cGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACt                                              <  1:2174563/60‑1 (MQ=255)
                                gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                                  >  1:4599508/1‑71 (MQ=255)
                                  gTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct                                <  1:747071/71‑1 (MQ=255)
                                    gCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcc                                 <  1:2590620/68‑1 (MQ=255)
                                     cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAg                                                               >  1:3616390/1‑37 (MQ=255)
                                       cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtc                           <  1:168697/71‑1 (MQ=255)
                                        gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                                           <  1:2787953/38‑1 (MQ=255)
                                         ttATCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACt                                                    <  1:3373230/43‑1 (MQ=12)
                                         ttATCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACt                                                    <  1:2325871/43‑1 (MQ=12)
                                          tATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTa                                                         >  1:5105221/1‑38 (MQ=255)
                                          tATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                       >  1:1171821/1‑56 (MQ=255)
                                           aTCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGAc                                         >  1:3205980/1‑53 (MQ=19)
                                           aTCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                                           <  1:1875241/35‑1 (MQ=255)
                                            tctgtcGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCg                       <  1:3323946/70‑1 (MQ=255)
                                            tctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc                        >  1:5193665/1‑69 (MQ=255)
                                             ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                       <  1:1298291/53‑1 (MQ=255)
                                             ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                       <  1:3387117/53‑1 (MQ=255)
                                             ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                       <  1:5300695/53‑1 (MQ=255)
                                             ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc                      >  1:5165428/1‑70 (MQ=255)
                                              tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGa                                                      >  1:1631215/1‑37 (MQ=255)
                                              tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                                                >  1:2146808/1‑43 (MQ=255)
                                              tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                                                >  1:2222665/1‑43 (MQ=255)
                                              tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                                                >  1:1441997/1‑43 (MQ=255)
                                              tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                                                >  1:802918/1‑43 (MQ=255)
                                              tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct                                >  1:725684/1‑59 (MQ=255)
                                              tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct                                >  1:1252817/1‑59 (MQ=255)
                                               ctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc                        >  1:1235824/1‑66 (MQ=255)
                                               ctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaaa                   >  1:5052580/1‑71 (MQ=255)
                                                tcGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCgatg                    <  1:761100/69‑4 (MQ=255)
                                                tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAc                  >  1:3511473/1‑71 (MQ=255)
                                                  gctgctTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTg                                      >  1:3583570/1‑49 (MQ=16)
                                                  gCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAcc                 <  1:2597733/70‑1 (MQ=255)
                                                  gCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAcc                 <  1:569964/70‑1 (MQ=255)
                                                     gATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAcc                 <  1:3422109/67‑1 (MQ=255)
                                                       ttGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTggg             >  1:5620596/1‑69 (MQ=255)
                                                       ttGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGc           <  1:1200017/71‑1 (MQ=255)
                                                         ggCGGTACATGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCg          <  1:1009608/70‑1 (MQ=255)
                                                             gTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCaa     <  1:3585810/71‑1 (MQ=255)
                                                               aCTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCa      <  1:663507/68‑1 (MQ=255)
                                                                cTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAAc   <  1:4644879/70‑1 (MQ=255)
                                                                cTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACt  <  1:163062/71‑1 (MQ=255)
                                                                 ttGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct                                >  1:1414845/1‑40 (MQ=255)
                                                                 ttGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct                                >  1:4451249/1‑40 (MQ=255)
                                                                 ttGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaa                    <  1:5615593/52‑1 (MQ=255)
                                                                 |                                                                     
ATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACT  >  Exported/832116‑832250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: