| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 832,404 | A→G | 6.9% | I288V (ATC→GTC) ‡ | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 832,404 | 0 | A | G | 6.9% | 332.7 / 10.3 | 117 | I288V (ATC→GTC) ‡ | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (55/53); new base G (6/2); total (61/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01 | |||||||||||
Only 100 of 133 total aligned reads displayed.
TGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGG > Exported/832334‑832474 | aCTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGc > 1:2624974/1‑70 (MQ=255) aCTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGc > 1:2640918/1‑70 (MQ=255) tcttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCaaa > 1:2717180/1‑71 (MQ=255) cttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCaaa < 1:2724908/70‑1 (MQ=255) cttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAAc > 1:1454583/1‑71 (MQ=255) cttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAAc > 1:3395995/1‑71 (MQ=255) aCTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCaa < 1:4275270/65‑1 (MQ=255) aCTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACtgtg > 1:2901675/1‑71 (MQ=255) aCTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACtgtg > 1:5422094/1‑71 (MQ=255) aCTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACtgt > 1:88103/1‑70 (MQ=255) cTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGc < 1:5286533/71‑1 (MQ=255) gAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGa > 1:5670754/1‑71 (MQ=255) aaGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCg < 1:1822553/69‑1 (MQ=255) aaGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCg < 1:1851614/69‑1 (MQ=255) aGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATc > 1:1294859/1‑71 (MQ=255) tGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGa < 1:517313/67‑1 (MQ=255) tGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCtg > 1:2701369/1‑71 (MQ=255) ttACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGaaa < 1:95249/47‑1 (MQ=255) ttACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACtgt < 1:617241/59‑1 (MQ=255) ttACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACtgt < 1:1713914/59‑1 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACTCTGAAATCGcc > 1:4828489/1‑50 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACTCTGAAATCGcc > 1:4834499/1‑50 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATc > 1:1235976/1‑47 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGcc > 1:2869033/1‑50 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCaaa > 1:1711066/1‑53 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCa > 1:5662090/1‑51 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCa < 1:5438222/51‑1 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCa < 1:3993389/51‑1 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCa < 1:3783935/51‑1 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggct < 1:2914552/70‑1 (MQ=255) aaaGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggctgg > 1:4894099/1‑70 (MQ=255) aaaGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggctgg > 1:2249693/1‑70 (MQ=255) aaGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggctgg < 1:4086787/69‑1 (MQ=255) ggACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCAcc > 1:1196310/1‑70 (MQ=255) ggACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCAcc > 1:4125620/1‑70 (MQ=255) gACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGa < 1:2448142/71‑1 (MQ=255) aCCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGaa < 1:2999605/71‑1 (MQ=255) aCCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGaa < 1:4646452/71‑1 (MQ=255) ccAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCaaa < 1:63704/44‑1 (MQ=255) ccAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGaaa < 1:4162029/71‑1 (MQ=255) aaaCTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggctggc < 1:2482576/61‑1 (MQ=255) aaaCTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCCCCGAAACa > 1:5118343/1‑70 (MQ=255) aaCTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAAc > 1:2172988/1‑41 (MQ=255) ttGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAgg < 1:669984/66‑1 (MQ=255) ttGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAgg < 1:3690683/66‑1 (MQ=255) ttGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAcc < 1:1910169/71‑1 (MQ=255) ccGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGAt < 1:3196267/71‑1 (MQ=255) tCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACa < 1:894360/58‑1 (MQ=255) tCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACa < 1:3756818/58‑1 (MQ=255) tCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGa < 1:3866823/67‑1 (MQ=255) cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCtg > 1:2958692/1‑40 (MQ=255) cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCtg > 1:2355399/1‑40 (MQ=255) cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGc > 1:2585835/1‑61 (MQ=255) cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCgg > 1:2155976/1‑71 (MQ=255) cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCgg > 1:2149322/1‑71 (MQ=255) acacCAGACTACGCTGAAATCGCCAAACTGTGCGAt > 1:4251374/1‑36 (MQ=255) acacCAGACTACGCTGAAATCGCCAAACTGTGCGAt > 1:3032130/1‑36 (MQ=255) acacCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGaaa < 1:2837562/54‑1 (MQ=255) acacCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGaaa < 1:2830678/54‑1 (MQ=255) acacCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAgg > 1:592428/1‑58 (MQ=255) acacCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGc < 1:5383525/71‑1 (MQ=255) acCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCaa < 1:3964059/71‑1 (MQ=255) ccAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggctgg < 1:4590889/43‑1 (MQ=255) ccAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAAc < 1:5030950/52‑1 (MQ=255) cAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggc > 1:5126283/1‑39 (MQ=255) cAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggc > 1:169297/1‑39 (MQ=255) cAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGTACCGAAAc > 1:532683/1‑51 (MQ=255) cAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAAc > 1:4357905/1‑51 (MQ=255) cAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATg > 1:5291713/1‑71 (MQ=255) cAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATg > 1:4531944/1‑71 (MQ=255) aGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATgg < 1:3169226/71‑1 (MQ=255) aGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATgg > 1:1044184/1‑71 (MQ=255) aGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATgg < 1:4082993/71‑1 (MQ=255) gACTACTCTGAAGTGGCCAAATTGTGCGACCAGTGGCTGGCACCGaaa > 1:457237/1‑48 (MQ=19) gACTACTCTGAAGTGGCCAAATTGTGCGACCAGTGGCTGGCACCGaaa > 1:5658483/1‑48 (MQ=19) gACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgc < 1:2195511/71‑1 (MQ=255) aCTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAcc < 1:2056672/56‑1 (MQ=255) aCTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAcc < 1:2506091/56‑1 (MQ=255) aCTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgcg > 1:2937726/1‑71 (MQ=255) aCTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgcg > 1:3295111/1‑71 (MQ=255) aCTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgcg > 1:3504627/1‑71 (MQ=255) aCTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgcg > 1:4369683/1‑71 (MQ=255) tACGCTGAAATCGCCAAACTGTGCGATCTGtggctgg < 1:2699977/37‑1 (MQ=255) tACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGa < 1:712533/43‑1 (MQ=255) tACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGa < 1:667677/43‑1 (MQ=255) tACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCt > 1:1171134/1‑71 (MQ=255) cGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTgg < 1:2331287/71‑1 (MQ=255) cGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTgg > 1:4875849/1‑71 (MQ=255) tcTGAAGTGGCCAAATTGTGCGACCAGTGGCTGGCACCGAAAc < 1:1324339/42‑1 (MQ=16) cTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGc < 1:3317750/58‑1 (MQ=255) tGAAGTGGCCAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCAct > 1:2389869/1‑48 (MQ=19) tGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATgg < 1:2759535/62‑1 (MQ=255) tGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCt < 1:471326/66‑1 (MQ=255) tGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGa > 1:4293305/1‑71 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAcc < 1:1202196/47‑1 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgcg > 1:4746651/1‑62 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAt < 1:2656352/70‑1 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATg < 1:4550437/71‑1 (MQ=255) aaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCg < 1:1224752/47‑1 (MQ=255) aTCGCCAAACTGTGCGATCTGTGGCTGGCCCCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggt > 1:1041489/1‑70 (MQ=255) | TGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGG > Exported/832334‑832474 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |