| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 189,592 | A→G | 21.1% | Q363Q (CAA→CAG) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 189,592 | 0 | A | G | 21.1% | 132.8 / 31.6 | 71 | Q363Q (CAA→CAG) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (27/29); new base G (8/7); total (35/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
ATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACG > Exported/189522‑189659 | aTCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCaa > 1:1999617/1‑71 (MQ=255)aTCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCaa > 1:3819833/1‑71 (MQ=255)aTCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCaa > 1:3298468/1‑71 (MQ=255) tCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAAt < 1:1594876/71‑1 (MQ=255) ccGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATc > 1:4838050/1‑71 (MQ=255) ccGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATc > 1:1319715/1‑71 (MQ=255) cGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATc > 1:3192380/1‑70 (MQ=255) gATTTACGAAGGTAAATGCGGTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTcca < 1:2364591/71‑3 (MQ=255) gATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGa > 1:3906376/1‑71 (MQ=255) acTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGAcc > 1:5670592/2‑71 (MQ=255) aCCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCca > 1:161151/1‑70 (MQ=255) ccAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATc < 1:1944296/63‑1 (MQ=255) ccAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGa > 1:5037802/1‑65 (MQ=255) ccAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGa > 1:1068464/1‑65 (MQ=255) ccAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCcaca < 1:4386824/71‑1 (MQ=255) ccAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCcac > 1:365569/1‑70 (MQ=255) ccAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCcac > 1:3364452/1‑70 (MQ=255) gaaggTAAATGCGGTATGAGCGGTGTCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACaa < 1:2288477/68‑1 (MQ=255) cAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaa < 1:1677336/71‑1 (MQ=255) aaggTAAATGCGGTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACaaa > 1:3386987/3‑71 (MQ=255) ggTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAAt < 1:4932725/71‑1 (MQ=255) tAAATGCGGTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACAAACTGc < 1:4873280/71‑1 (MQ=255) aaaaGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCt > 1:1720803/1‑71 (MQ=255) aaGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTgg < 1:1277332/71‑1 (MQ=255) aaGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTgg > 1:4387942/1‑71 (MQ=255) tGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa > 1:1664180/1‑62 (MQ=255) cggTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACaa > 1:4637517/2‑60 (MQ=255) cggTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACACAGTCCACTCACaa > 1:808938/2‑60 (MQ=255) gTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGgcggcg > 1:765353/1‑71 (MQ=255) aTGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGtt > 1:3310451/1‑71 (MQ=255) tGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGtt < 1:4972451/70‑1 (MQ=255) tGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTa < 1:4594300/71‑1 (MQ=255) gAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGtt < 1:3147560/69‑1 (MQ=255) gcggcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:5024137/71‑1 (MQ=255) gcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAg > 1:1361244/1‑71 (MQ=255) gcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAg > 1:5478784/1‑71 (MQ=255) tgtTGCGGGAAAAGAGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCa < 1:4531633/63‑1 (MQ=255) ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAAt < 1:193018/43‑1 (MQ=255) ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATTCTGGCGGCGTTATCGCAGGCTTCGCt < 1:1891243/70‑1 (MQ=255) ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCt < 1:1688458/70‑1 (MQ=255) ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCt < 1:4195592/70‑1 (MQ=255) cGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGAt < 1:1403813/70‑1 (MQ=255) gggAAAAGTGATCTTCGAAACGCAATCGACCCACaa < 1:3210490/36‑1 (MQ=255) gggAAAAGTGATCTTCGAAACGCAATCGACCCACaa < 1:1037178/36‑1 (MQ=255) gggAAAAGTGATCTTCGAAACGCAATCGACCCACaa < 1:396692/36‑1 (MQ=255) gggAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATcc < 1:4567406/71‑1 (MQ=255) ggaaaGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCcac > 1:2759123/3‑71 (MQ=255) ggaaaGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCca > 1:1646249/3‑70 (MQ=255) gAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCca > 1:2249731/1‑70 (MQ=255) gAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCca > 1:5473956/1‑70 (MQ=255) aaaGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCcacgt > 1:3790835/1‑69 (MQ=255) aaaGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATTCACAt > 1:2461359/1‑71 (MQ=255) aaaGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACAt > 1:1352376/1‑71 (MQ=255) aaGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGtt < 1:3372895/71‑1 (MQ=255) aGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTa < 1:3760592/71‑1 (MQ=255) aGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:5328848/71‑1 (MQ=255) aGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:4224866/71‑1 (MQ=255) aGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:1140448/71‑1 (MQ=255) gTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTaa < 1:1430545/71‑1 (MQ=255) gTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:1564498/70‑1 (MQ=255) tGATCTTCGAAACGCAATCGACCCACAAAATGCTgg > 1:1165198/1‑36 (MQ=255) cTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTa < 1:806274/40‑1 (MQ=255) cTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg > 1:1203878/1‑55 (MQ=255) cTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg > 1:5611158/1‑55 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCg < 1:4468663/68‑1 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAg > 1:2828260/1‑70 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAg > 1:2389013/1‑70 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATAGCAGGCTTCGCTGATCCACATTAAAGGCGAg < 1:3524215/70‑1 (MQ=255) gAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTa > 1:4136647/1‑71 (MQ=255) aaaCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTc < 1:1094194/46‑1 (MQ=255) aaaCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTc < 1:635745/46‑1 (MQ=255) aaCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATg < 1:3577523/71‑1 (MQ=255) aCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGa > 1:4396090/1‑71 (MQ=255) gCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGAc > 1:1814148/1‑70 (MQ=255) aTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACg > 1:3528236/1‑68 (MQ=255) | ATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACG > Exported/189522‑189659 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |