| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 189,605 | A→C | 20.6% | M368L (ATG→CTG) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 189,605 | 0 | A | C | 20.6% | 104.5 / 33.6 | 63 | M368L (ATG→CTG) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (21/29); new base C (8/5); total (29/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01 | |||||||||||
GGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGA > Exported/189536‑189675 | ggTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAAt < 1:4932725/71‑1 (MQ=255) tAAATGCGGTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACAAACTGc < 1:4873280/71‑1 (MQ=255) aaaaGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCt > 1:1720803/1‑71 (MQ=255) aaGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTgg < 1:1277332/71‑1 (MQ=255) aaGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTgg > 1:4387942/1‑71 (MQ=255) tGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa > 1:1664180/1‑62 (MQ=255) gTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGgcggcg > 1:765353/1‑71 (MQ=255) aTGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGtt > 1:3310451/1‑71 (MQ=255) tGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGtt < 1:4972451/70‑1 (MQ=255) tGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTa < 1:4594300/71‑1 (MQ=255) gAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGtt < 1:3147560/69‑1 (MQ=255) gcggcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:5024137/71‑1 (MQ=255) gcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAg > 1:5478784/1‑71 (MQ=255) gcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAg > 1:1361244/1‑71 (MQ=255) tgtTGCGGGAAAAGAGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCa < 1:4531633/63‑1 (MQ=255) ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAAt < 1:193018/43‑1 (MQ=255) ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATTCTGGCGGCGTTATCGCAGGCTTCGCt < 1:1891243/70‑1 (MQ=255) ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCt < 1:4195592/70‑1 (MQ=255) ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCt < 1:1688458/70‑1 (MQ=255) cGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGAt < 1:1403813/70‑1 (MQ=255) gggAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATcc < 1:4567406/71‑1 (MQ=255) ggaaaGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCcac > 1:2759123/3‑71 (MQ=255) ggaaaGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCca > 1:1646249/3‑70 (MQ=255) gAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCca > 1:2249731/1‑70 (MQ=255) gAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCca > 1:5473956/1‑70 (MQ=255) aaaGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCcacgt > 1:3790835/1‑69 (MQ=255) aaaGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATTCACAt > 1:2461359/1‑71 (MQ=255) aaaGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACAt > 1:1352376/1‑71 (MQ=255) aaGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGtt < 1:3372895/71‑1 (MQ=255) aGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTa < 1:3760592/71‑1 (MQ=255) aGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:5328848/71‑1 (MQ=255) aGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:1140448/71‑1 (MQ=255) aGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:4224866/71‑1 (MQ=255) gTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTaa < 1:1430545/71‑1 (MQ=255) gTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:1564498/70‑1 (MQ=255) tGATCTTCGAAACGCAATCGACCCACAAAATGCTgg > 1:1165198/1‑36 (MQ=255) cTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTa < 1:806274/40‑1 (MQ=255) cTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg > 1:1203878/1‑55 (MQ=255) cTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg > 1:5611158/1‑55 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCg < 1:4468663/68‑1 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAg > 1:2828260/1‑70 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAg > 1:2389013/1‑70 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATAGCAGGCTTCGCTGATCCACATTAAAGGCGAg < 1:3524215/70‑1 (MQ=255) gAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTa > 1:4136647/1‑71 (MQ=255) aaaCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTc < 1:635745/46‑1 (MQ=255) aaaCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTc < 1:1094194/46‑1 (MQ=255) aaCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATg < 1:3577523/71‑1 (MQ=255) aCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGa > 1:4396090/1‑71 (MQ=255) gCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGAc > 1:1814148/1‑70 (MQ=255) aTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACg > 1:3528236/1‑68 (MQ=255) tCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCt < 1:606893/36‑1 (MQ=255) gACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAgg > 1:969445/1‑71 (MQ=255) gACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGAACCACAtt > 1:3021550/1‑49 (MQ=255) cacaAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTa > 1:1910623/1‑46 (MQ=16) acaAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATc > 1:610880/1‑38 (MQ=16) acaAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCcac > 1:1381805/1‑41 (MQ=19) acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAg < 1:4881224/53‑1 (MQ=255) acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa > 1:4640438/1‑62 (MQ=255) acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCttt < 1:1393737/71‑1 (MQ=255) acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCttt < 1:5283223/71‑1 (MQ=255) acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCtt > 1:165013/1‑70 (MQ=255) caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACg < 1:5583990/59‑1 (MQ=255) caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTa < 1:5347006/71‑1 (MQ=255) caAAATGCTGGCGGCGGTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGAc < 1:3759399/58‑1 (MQ=255) aaaaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCca < 1:4970359/38‑1 (MQ=255) aaaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACg < 1:4168114/57‑1 (MQ=255) aaaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa > 1:4537899/1‑59 (MQ=255) aaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTaa < 1:4526349/42‑1 (MQ=255) aTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGa < 1:1993556/71‑1 (MQ=255) | GGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGA > Exported/189536‑189675 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |