| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 189,622 | G→T | 21.7% | S373S (TCG→TCT) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 189,622 | 0 | G | T | 21.7% | 170.2 / 27.6 | 69 | S373S (TCG→TCT) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (23/31); new base T (9/6); total (32/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATG > Exported/189552‑189688 | gcggcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:5024137/71‑1 (MQ=255) gcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAg > 1:1361244/1‑71 (MQ=255) gcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAg > 1:5478784/1‑71 (MQ=255) tgtTGCGGGAAAAGAGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCa < 1:4531633/63‑1 (MQ=255) ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATTCTGGCGGCGTTATCGCAGGCTTCGCt < 1:1891243/70‑1 (MQ=255) ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCt < 1:1688458/70‑1 (MQ=255) ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCt < 1:4195592/70‑1 (MQ=255) cGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGAt < 1:1403813/70‑1 (MQ=255) gggAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATcc < 1:4567406/71‑1 (MQ=255) ggaaaGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCcac > 1:2759123/3‑71 (MQ=255) ggaaaGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCca > 1:1646249/3‑70 (MQ=255) gAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCca > 1:2249731/1‑70 (MQ=255) gAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCca > 1:5473956/1‑70 (MQ=255) aaaGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCcacgt > 1:3790835/1‑69 (MQ=255) aaaGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATTCACAt > 1:2461359/1‑71 (MQ=255) aaaGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACAt > 1:1352376/1‑71 (MQ=255) aaGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGtt < 1:3372895/71‑1 (MQ=255) aGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTa < 1:3760592/71‑1 (MQ=255) aGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:5328848/71‑1 (MQ=255) aGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:4224866/71‑1 (MQ=255) aGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:1140448/71‑1 (MQ=255) gTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTaa < 1:1430545/71‑1 (MQ=255) gTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:1564498/70‑1 (MQ=255) cTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg > 1:5611158/1‑55 (MQ=255) cTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTg > 1:1203878/1‑55 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCg < 1:4468663/68‑1 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑g > 1:2389013/1‑70 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑g > 1:2828260/1‑70 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATAGCAGGCTTCGCTGATCCACATTAAAGGCGA‑g < 1:3524215/70‑1 (MQ=255) gAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTa > 1:4136647/1‑71 (MQ=255) aaaCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTc < 1:635745/46‑1 (MQ=255) aaaCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTc < 1:1094194/46‑1 (MQ=255) aaCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATg < 1:3577523/71‑1 (MQ=255) aCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGa > 1:4396090/1‑71 (MQ=255) gCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGAc > 1:1814148/1‑70 (MQ=255) aTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACg > 1:3528236/1‑68 (MQ=255) tCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCt < 1:606893/36‑1 (MQ=255) gACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAgg > 1:969445/1‑71 (MQ=255) gACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGAACCACAtt > 1:3021550/1‑49 (MQ=255) cacaAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTa > 1:1910623/1‑46 (MQ=16) acaAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATc > 1:610880/1‑38 (MQ=16) acaAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCcac > 1:1381805/1‑41 (MQ=19) acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑g < 1:4881224/53‑1 (MQ=255) acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa > 1:4640438/1‑62 (MQ=255) acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCttt < 1:1393737/71‑1 (MQ=255) acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCttt < 1:5283223/71‑1 (MQ=255) acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCtt > 1:165013/1‑70 (MQ=255) caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACg < 1:5583990/59‑1 (MQ=255) caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTa < 1:5347006/71‑1 (MQ=255) caAAATGCTGGCGGCGGTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGAc < 1:3759399/58‑1 (MQ=255) aaaaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCca < 1:4970359/38‑1 (MQ=255) aactGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAAc < 1:3267072/68‑1 (MQ=255) aaaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACg < 1:4168114/57‑1 (MQ=255) aaaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa > 1:4537899/1‑59 (MQ=255) actGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAAc > 1:2664385/3‑70 (MQ=255) aaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTaa < 1:4526349/42‑1 (MQ=255) ctGCTGGCGGCGTTCTCTCAGGCTTCCATGATCca > 1:821736/2‑35 (MQ=16) aTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGa < 1:1993556/71‑1 (MQ=255) tGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTaaa > 1:384446/1‑41 (MQ=255) tGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAGcc > 1:5052938/1‑71 (MQ=9) gcggcgTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAGCCta < 1:3187422/71‑2 (MQ=9) gcggcgTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAGCCta < 1:1625746/71‑2 (MQ=9) cggcgTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCttt < 1:3467769/71‑1 (MQ=255) gTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTatga < 1:1687361/71‑1 (MQ=255) aTCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa > 1:374156/1‑43 (MQ=255) aTCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTatgatg > 1:5344576/1‑70 (MQ=255) aTCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTatgatg > 1:200255/1‑70 (MQ=255) tCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa < 1:2421135/42‑1 (MQ=255) tCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa < 1:676933/42‑1 (MQ=255) tCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa < 1:4873787/42‑1 (MQ=255) gCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAaga > 1:3115107/1‑42 (MQ=255) gCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAaga > 1:952053/1‑42 (MQ=255) gCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTat > 1:2311735/1‑63 (MQ=255) | GCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATG > Exported/189552‑189688 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |